超声微泡介导质粒转染对人肝癌HepG2细胞迁移、侵袭及克隆能力的影响
发布时间：2018-09-07 21:52
【摘要】：目的探讨超声微泡造影剂介导miRNA质粒转染人肝癌HepG2细胞后迁移、侵袭及克隆能力的改变。方法在前期实验所筛选的最佳超声微泡转染条件下,转染目的基因反义miR-21/221、miR-199a进入人肝癌HepG2细胞,划痕实验、Transwell实验检测细胞迁移、侵袭能力,软琼脂克隆形成实验检测细胞克隆能力。结果转染目的质粒后,细胞的迁移、侵袭能力及克隆能力与对照组比较均显著受到抑制(P0.05),其中以miR-199a质粒组的抑制效果最佳(相对细胞迁移率31.05%;平均视野侵袭细胞数38.67±4.51;克隆数105.67±5.86),与其它目的质粒组比较,差异均有统计学意义(P0.05)。结论通过分析miRNAs对人肝癌HepG2细胞部分细胞功能的影响,为肝癌的基因治疗提供新的靶点及思路。
[Abstract]:Objective to investigate the migration, invasion and clone ability of human hepatocellular carcinoma (HepG2) cells transfected with miRNA plasmid by ultrasound microbubble contrast medium. Methods under the optimal ultrasound microbubble transfection conditions, the target gene antisense miR-21/221,miR-199a was transfected into human hepatoma HepG2 cells. Soft Agar Clone formation Test was used to detect the ability of cell clone. Results after transfection of the target plasmid, cell migration was observed. The invasiveness and clone ability of miR-199a plasmid group were significantly inhibited compared with the control group (P0.05). The inhibitory effect of miR-199a plasmid group was the best (relative cell migration rate 31.05; average visual field invasion cell number 38.67 卤4.51; clone number 105.67 卤5.86). The difference was statistically significant (P0.05). Conclusion by analyzing the effect of miRNAs on the function of human hepatoma HepG2 cells, we can provide new targets and ideas for gene therapy of HCC.
【作者单位】： 暨南大学医学院附属广州市红会医院超声诊断科;广东药学院附属广钢医院超声诊断科;
【基金】：广东省科技计划项目立项(编号:2016A020215015)
【分类号】：R735.7
[Abstract]:Objective to investigate the migration, invasion and clone ability of human hepatocellular carcinoma (HepG2) cells transfected with miRNA plasmid by ultrasound microbubble contrast medium. Methods under the optimal ultrasound microbubble transfection conditions, the target gene antisense miR-21/221,miR-199a was transfected into human hepatoma HepG2 cells. Soft Agar Clone formation Test was used to detect the ability of cell clone. Results after transfection of the target plasmid, cell migration was observed. The invasiveness and clone ability of miR-199a plasmid group were significantly inhibited compared with the control group (P0.05). The inhibitory effect of miR-199a plasmid group was the best (relative cell migration rate 31.05; average visual field invasion cell number 38.67 卤4.51; clone number 105.67 卤5.86). The difference was statistically significant (P0.05). Conclusion by analyzing the effect of miRNAs on the function of human hepatoma HepG2 cells, we can provide new targets and ideas for gene therapy of HCC.
【作者单位】： 暨南大学医学院附属广州市红会医院超声诊断科;广东药学院附属广钢医院超声诊断科;
【基金】：广东省科技计划项目立项(编号:2016A020215015)
【分类号】：R735.7
【参考文献】
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