KLF15在人肺腺癌中表达的临床意义

发布时间：2018-09-08 16:32

【摘要】：目的:Krüppel样因子15(Krüppel-like factor 15,KLF15)参与肿瘤的增殖、侵袭和转移,但在肺腺癌中的表达和作用尚不明确。本研究探索KLF15蛋白在肺腺癌患者中的表达及其临床意义。方法:收集我院4例肺腺癌组织及匹配的癌旁组织,Western blot分析这些组织中KLF15蛋白表达的差异。同时收集我院72例肺腺癌患者的肿瘤组织标本及临床资料,免疫组织化学染色分析患者肺癌标本中KLF15蛋白的表达,分析其表达与患者临床特征包括预后的关系。另外,采用质粒转染的方法上调肺腺癌A549细胞中KLF15的表达,CCK-8法分析KLF15蛋白对肺腺癌细胞增殖能力的影响。结果:在4例肺腺癌及匹配的癌旁组织中,肺腺癌组织中KLF15蛋白表明显低于癌旁组织。在72例肺腺癌病理标本中,KLF15蛋白低表达或不表达者53例,占73.6%,KLF15高表达者19例,占26.4%。KLF15蛋白高表达的患者,其5年生存率明显优于KLF15低表达的患者(P0.01);KLF15蛋白表达与肺腺癌患者的病理分期(P0.01)及T分期明显相关(P0.01),KLF15蛋白低表达是肺腺癌患者不良预后的重要危险因素。上调肺腺癌细胞中KLF15蛋白的表达可明显抑制肺腺癌细胞的生长。结论:KLF15抑制肺腺癌细胞的生长,有望成为肺腺癌患者的治疗靶点和预后分子标志物。
[Abstract]:Objective Kr 眉 Kr 眉 ppel like factor 15 (Kr 眉 ppel-like factor 15 KLF15) is involved in the proliferation, invasion and metastasis of tumor, but the expression and role of Kr 眉 Kr 眉 KLF15 in lung adenocarcinoma is unclear. This study was to investigate the expression and clinical significance of KLF15 protein in patients with lung adenocarcinoma. Methods: the expression of KLF15 protein in 4 cases of lung adenocarcinoma and matched adjacent tissues were analyzed by Western blot. At the same time, the tumor tissue and clinical data of 72 patients with lung adenocarcinoma in our hospital were collected. The expression of KLF15 protein was analyzed by immunohistochemical staining, and the relationship between the expression of KLF15 protein and the clinical features, including prognosis, was analyzed. In addition, the expression of KLF15 in lung adenocarcinoma A549 cells was up-regulated by plasmid transfection. The effect of KLF15 protein on the proliferation of lung adenocarcinoma A549 cells was analyzed by CCK-8 method. Results: in 4 cases of lung adenocarcinoma and matched paracancerous tissues, the expression of KLF15 protein in lung adenocarcinoma was significantly lower than that in paracancerous tissues. In 72 cases of lung adenocarcinoma, there were 53 cases with low or no expression of KLF15 protein, 19 cases with high expression of KLF15 protein in 73.6 cases, and 19 cases with high expression of 26.4%.KLF15 protein. The 5-year survival rate was significantly better than that in patients with low expression of KLF15 (P0.01). The expression of KLF15 protein was significantly correlated with pathological stage (P0.01) and T stage (P0.01) of patients with lung adenocarcinoma, which was an important risk factor for poor prognosis of patients with lung adenocarcinoma. Upregulation of KLF15 protein in lung adenocarcinoma cells significantly inhibited the growth of lung adenocarcinoma cells. Conclusion KLF15 inhibits the growth of lung adenocarcinoma cells and may be a therapeutic target and prognostic molecular marker in lung adenocarcinoma patients.
【作者单位】：暨南大学附属第一医院呼吸内科;
【基金】：国家自然科学基金青年基金资助项目(No.81302030)
【分类号】：R734.2

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