ESM-1在低氧环境下胃腺癌细胞中的表达及意义

发布时间：2018-09-14 15:49

【摘要】：目的研究用二氯化钴(CoCl_2)模拟胃癌细胞缺氧微环境的合适浓度,探讨内皮细胞特异性分子-1(ESM-1)在胃腺癌SGC-7901/BGC-823细胞缺氧环境中的表达情况。方法以两种人胃腺癌细胞(SGC-7901/BGC-823)为研究对象,分别用不同浓度二氯化钴(CoCl_2)处理人胃腺癌细胞后,用四唑化合物(MTS)法检测CoCl_2对SGC-7901和BGC-823细胞增殖的影响;Western blot分析不同浓度CoCl_2处理的人胃腺癌SGC-7901和BGC-823细胞中低氧诱导因子-1α(HIF-1α)和ESM-1表达的变化。结果≤0.6 mmol/L浓度的CoCl_2对胃腺癌SGC-7901和BGC-823细胞的增殖和细胞活力没有明显影响。在0.5、0.6 mmol/L CoCl_2浓度时,HIF-1α蛋白表达明显增加,但ESM-1表达明显下降。结论0.5、0.6 mmol/L CoCl_2可以模拟SGC-7901/BGC-823细胞的缺氧环境,在CoCl_20.5、0.6 mmol/L模拟细胞缺氧环境中,ESM-1的表达量与HIF-1α呈负相关。
[Abstract]:Objective to study the suitable concentration of cobalt dichloride (CoCl_2) in anoxic microenvironment of gastric cancer cells and to investigate the expression of endothelial cell-specific molecule 1 (ESM-1) in SGC-7901/BGC-823 cells of gastric adenocarcinoma. Methods two kinds of human gastric adenocarcinoma cells (SGC-7901/BGC-823) were treated with different concentrations of cobalt dichloride (CoCl_2). The effects of CoCl_2 on the proliferation of SGC-7901 and BGC-823 cells were detected by (MTS) assay. The expression of hypoxia inducible factor 1 伪 (HIF-1 伪) and ESM-1 in SGC-7901 and BGC-823 cells treated with different concentrations of CoCl_2 were analyzed by Western blot. Results CoCl_2 with concentration 鈮，

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