载阿霉素介孔二氧化硅纳米粒对人舌癌Tca-8113细胞增殖及凋亡的影响
发布时间：2018-09-15 07:19
【摘要】：目的:探讨载阿霉素介孔二氧化硅纳米粒(MSNs@DOX)对体外培养的人舌癌Tca-8113细胞增殖及凋亡的作用。方法:透射电镜(TEM)和氮吸附法对介孔二氧化硅纳米粒(MSNs)进行表征;紫外分光光度计检测阿霉素(DOX)释放行为;MTT法检测细胞增殖抑制作用;Hochest33342染色、流式细胞术检测细胞凋亡和周期分布;Western blot检测P53、Bcl-2、Bax和Caspase-3蛋白表达。结果:MSNs形态规则,分布均一,平均粒径、介孔孔径、比表面积和孔容分别为约30nm,19.59682nm、193.4296m2/g、0.947625cm3/g;MSNs细胞相容性良好;与单独DOX相比,载药组的细胞增殖抑制率及凋亡率更显著,且呈一定MSNs浓度依赖性(P0.05);细胞明显阻滞于G0/G1期;P53、Bax、Caspase-3表达显著升高,Bcl-2表达显著降低。结论:该载药纳米传输系统有望用于舌癌治疗。
[Abstract]:Aim: to investigate the effect of doxorubicin loaded mesoporous silica nanoparticles (MSNs@DOX) on proliferation and apoptosis of human tongue cancer Tca-8113 cells in vitro. Methods: the (MSNs) of mesoporous silica nanoparticles was characterized by transmission electron microscopy (TEM) and nitrogen adsorption, and the release behavior of doxorubicin (DOX) was detected by UV spectrophotometer. Apoptosis and cell cycle distribution were detected by flow cytometry. Western blot was used to detect the expression of Bcl-2 Bax and Caspase-3 protein. Results the morphology, distribution, mean particle size, mesoporous pore size, specific surface area and pore volume were about 30 nm ~ (-1), 19.59682 nm ~ (-1), 193.4296 m ~ (2 /) 路g ~ (-1) ~ 0. 47625 cm ~ (3) / g ~ (-1), respectively. Compared with DOX alone, the cell proliferation inhibition rate and apoptosis rate of drug loaded group were more significant. The expression of Caspase-3 was significantly increased in the G0/G1 phase and the expression of Bcl-2 was significantly decreased. Conclusion: the drug-loaded nano-delivery system is expected to be used in the treatment of tongue cancer.
【作者单位】： 兰州大学口腔医学院口腔修复科;中国科学院兰州化学物理研究所固体润滑国家重点实验室;
【基金】：中央高校自由探索-面上项目(编号:lzujbky-2015-293) 甘肃省卫生行业科研计划管理项目(编号:GWGL2014-06)
【分类号】：R739.86
[Abstract]:Aim: to investigate the effect of doxorubicin loaded mesoporous silica nanoparticles (MSNs@DOX) on proliferation and apoptosis of human tongue cancer Tca-8113 cells in vitro. Methods: the (MSNs) of mesoporous silica nanoparticles was characterized by transmission electron microscopy (TEM) and nitrogen adsorption, and the release behavior of doxorubicin (DOX) was detected by UV spectrophotometer. Apoptosis and cell cycle distribution were detected by flow cytometry. Western blot was used to detect the expression of Bcl-2 Bax and Caspase-3 protein. Results the morphology, distribution, mean particle size, mesoporous pore size, specific surface area and pore volume were about 30 nm ~ (-1), 19.59682 nm ~ (-1), 193.4296 m ~ (2 /) 路g ~ (-1) ~ 0. 47625 cm ~ (3) / g ~ (-1), respectively. Compared with DOX alone, the cell proliferation inhibition rate and apoptosis rate of drug loaded group were more significant. The expression of Caspase-3 was significantly increased in the G0/G1 phase and the expression of Bcl-2 was significantly decreased. Conclusion: the drug-loaded nano-delivery system is expected to be used in the treatment of tongue cancer.
【作者单位】： 兰州大学口腔医学院口腔修复科;中国科学院兰州化学物理研究所固体润滑国家重点实验室;
【基金】：中央高校自由探索-面上项目(编号:lzujbky-2015-293) 甘肃省卫生行业科研计划管理项目(编号:GWGL2014-06)
【分类号】：R739.86
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