胰腺癌中低氧诱导因子-1α和轴突诱导因子4D的表达及其临床意义
发布时间：2018-09-18 13:03
【摘要】：目的:研究低氧诱导因子-1α(hypoxia inducible factor-1α,HIF-1α)和轴突诱导因子4D(Semaphorin 4D,Sema4D)在胰腺癌组织中的表达,探讨两者之间的相关性及其临床意义。方法:采用免疫组织化学(immunohistochemistry,SP)法检测天津医科大学附属肿瘤医院和邯郸市中心医院82例胰腺癌组织和相应癌旁组织中HIF-1α和Sema4D的表达,采用Chi-square检验分析HIF-1α和Sema4D在不同组织中的表达差异,采用Chi-square检验分析HIF-1α和Sema4D与患者的性别、年龄、分化程度、肿瘤大小、胰腺癌分期、淋巴结转移、神经受侵、脉管内瘤栓等的相关性。HIF-1α和Sema4D在胰腺癌组织中的表达的相关性采用Mc Nemar检验分析其相关性。HIF-1α和Sema4D对胰腺癌患者生存率的影响分析采用Kaplan-meier法,生存曲线的比较采用Log-rank检验。Cox回归模型多因素分析方法,分析HIF-1α和Sema4D是否胰腺癌独立预后因素。结果:HIF-1α和Sema4D的阳性表达主要在细胞质内,呈棕黄色颗粒。HIF-1α和Sema4D在胰腺癌组织中的阳性表达率分别为52.4%(43/82)和51.2%(42/82),显著高于两者在癌旁组织的表达,差异有统计学意义(P0.05)。HIF-1α的异常表达与胰腺癌分期(χ~2=11.398,P=0.000)、淋巴结转移(χ~2=20.341,P=0.000)、神经受侵(χ~2=23.478,P=0.000)、脉管内瘤栓(χ~2=8.281,P=0.000)相关,Sema4D的异常表达与胰腺癌分期(χ~2=7.212,P=0.007)、淋巴结转移(χ~2=60129,P=0.013)、神经受侵(χ~2=25.794,P=0.000)、脉管内瘤栓(χ~2=6.678,P=0.010)相关。HIF-1α和Sema4D的表达之间存在正相关性(κ=0.438,P=0.000)。单因素生存分析HIF-1α(χ~2=12.098,P=0.001)、Sema4D(χ~2=9.644,P=0.002)是影响胰腺癌的预后因素,Cox分析显示2者均不是影响胰腺癌的独立预后因素。结论:胰腺癌组织中存在HIF-1α和Sema4D表达的上调,两者具有明显相关性,与胰腺癌淋巴结转移、神经受侵、脉管内瘤栓有关,HIF-1α、Sema4D的表达可能提示胰腺癌患者分期较晚和预后不良。
[Abstract]:Objective: to investigate the expression of hypoxia inducible factor 1 伪 (hypoxia inducible factor-1 伪 (HIF-1 伪) and axon inducible factor 4D (Semaphorin 4D) in pancreatic carcinoma, and to explore the correlation between them and their clinical significance. Methods: immunohistochemical method (immunohistochemistry,SP) was used to detect the expression of HIF-1 伪 and Sema4D in 82 cases of pancreatic carcinoma and adjacent tissues in affiliated Cancer Hospital of Tianjin Medical University and Handan Central Hospital. The expression of HIF-1 伪 and Sema4D in different tissues was analyzed by Chi-square test, and the sex, age, differentiation, tumor size, stage of pancreatic cancer, lymph node metastasis, nerve invasion were analyzed by Chi-square test. Correlation of tumor embolus et al. Expression of HIF-1 伪 and Sema4D in Pancreatic carcinoma. The correlation between HIF-1 伪 and Sema4D in pancreatic carcinoma was analyzed by Mc Nemar test. The effect of HIF-1 伪 and Sema4D on the survival rate of pancreatic cancer patients was analyzed by Kaplan-meier method. The survival curve was compared by Log-rank test. Cox regression model was used to analyze whether HIF-1 伪 and Sema4D were independent prognostic factors of pancreatic cancer. Results the positive expression rates of Sema4D and HIF-1 伪 were 52.4% (43 / 82) and 51.2% (42 / 82) in pancreatic carcinoma, respectively, which were significantly higher than those in paracancerous tissues, and the positive rates of HIF-1 伪 and Sema4D were 52.4% (43 / 82) and 51.2% (42 / 82), respectively. 宸紓鏈夌粺璁″鎰忎箟(P0.05).HIF-1伪鐨勫紓甯歌〃杈句笌鑳拌吅鐧屽垎鏈，
本文编号：2248008
[Abstract]:Objective: to investigate the expression of hypoxia inducible factor 1 伪 (hypoxia inducible factor-1 伪 (HIF-1 伪) and axon inducible factor 4D (Semaphorin 4D) in pancreatic carcinoma, and to explore the correlation between them and their clinical significance. Methods: immunohistochemical method (immunohistochemistry,SP) was used to detect the expression of HIF-1 伪 and Sema4D in 82 cases of pancreatic carcinoma and adjacent tissues in affiliated Cancer Hospital of Tianjin Medical University and Handan Central Hospital. The expression of HIF-1 伪 and Sema4D in different tissues was analyzed by Chi-square test, and the sex, age, differentiation, tumor size, stage of pancreatic cancer, lymph node metastasis, nerve invasion were analyzed by Chi-square test. Correlation of tumor embolus et al. Expression of HIF-1 伪 and Sema4D in Pancreatic carcinoma. The correlation between HIF-1 伪 and Sema4D in pancreatic carcinoma was analyzed by Mc Nemar test. The effect of HIF-1 伪 and Sema4D on the survival rate of pancreatic cancer patients was analyzed by Kaplan-meier method. The survival curve was compared by Log-rank test. Cox regression model was used to analyze whether HIF-1 伪 and Sema4D were independent prognostic factors of pancreatic cancer. Results the positive expression rates of Sema4D and HIF-1 伪 were 52.4% (43 / 82) and 51.2% (42 / 82) in pancreatic carcinoma, respectively, which were significantly higher than those in paracancerous tissues, and the positive rates of HIF-1 伪 and Sema4D were 52.4% (43 / 82) and 51.2% (42 / 82), respectively. 宸紓鏈夌粺璁″鎰忎箟(P0.05).HIF-1伪鐨勫紓甯歌〃杈句笌鑳拌吅鐧屽垎鏈，
本文编号：2248008
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