MicroRNA-300在胆管癌患者血清中的表达及其临床意义

发布时间：2018-09-19 07:01

【摘要】：目的探讨microRNA-300(mi R-300)在胆管癌患者及健康对照血清中的表达及其在诊断和判断预后方面的临床价值。方法选取107例胆管癌患者和107例健康对照的血清标本,提取血清总RNA,通过实时定量PCR检测血清mi R-300的表达水平,并绘制ROC曲线检测mi R-300在胆管癌中的诊断价值;采用χ2检验、Kaplan-Meier法分析mi R-300的表达水平与胆管癌患者临床特征及患者5年总体生存率之间的关系。结果胆管癌患者血清中mi R-300的表达水平高于健康对照者(P0.05),且在术后降低;mi R-300为胆管癌有效的诊断指标;血清mi R-300的表达与胆管癌患者的临床分期密切相关(P=0.010),而与年龄、性别及分化程度无相关;血清mi R-300高表达的胆管癌患者5年总体生存率低于血清mi R-300低表达患者。结论胆管癌患者血清中mi R-300表达增高,是胆管癌临床诊断和判断预后的有效分子指标,具有重要的临床应用价值。
[Abstract]:Objective to investigate the expression of microRNA-300 (mi R-300) in serum of patients with cholangiocarcinoma and healthy controls and its clinical value in diagnosis and prognosis. Methods Serum total RNA, was extracted from 107 patients with cholangiocarcinoma and 107 healthy controls. The expression of serum mi R-300 was detected by real-time quantitative PCR, and the diagnostic value of mi R-300 in cholangiocarcinoma was determined by ROC curve. The relationship between the expression of mi R-300 and the clinical features and 5-year overall survival rate of patients with cholangiocarcinoma was analyzed by using Kaplan-Meier method with 蠂 2 test. Results the expression of mi R-300 in serum of patients with cholangiocarcinoma was higher than that of healthy controls (P0.05), and the decrease of serum mi R-300 was an effective diagnostic index for cholangiocarcinoma after operation (P0.05), and the expression of serum mi R-300 was closely related to the clinical stage of patients with cholangiocarcinoma (P0. 010), but it was associated with age. The 5-year overall survival rate of cholangiocarcinoma patients with high expression of serum mi R-300 was lower than that of patients with low expression of serum mi R-300. Conclusion the increased expression of mi R-300 in serum of patients with cholangiocarcinoma is an effective molecular marker for clinical diagnosis and prognosis of cholangiocarcinoma, and has important clinical application value.
【作者单位】：西南医科大学附属医院检验科;西南医科大学附属医院核医学科;
【基金】：泸州医学院科研课题青年基金(No:2012QN-50)
【分类号】：R735.8

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