大肠癌石蜡包埋组织中MiR-21表达变化及意义

发布时间：2018-10-08 11:03

【摘要】：目的观察大肠癌石蜡包埋组织中微小RNA-21(miR-21)的表达变化,并探讨其临床意义。方法从40例大肠癌患者石蜡包埋的癌组织和癌旁5 cm范围外的正常黏膜组织中抽提RNA,采用反转录实时荧光定量PCR法检测miR-21,并分析癌组织中miR-21表达与大肠癌患者临床病理特征的关系。结果 miR-21在癌组织中相对表达量为-4.28±1.67,在癌旁正常组织为-2.05±1.85,miR-21在癌组织中的相对表达量较癌旁正常黏膜组织升高(P0.05)。在不同性别、肿瘤形态及发病部位患者的癌组织中miR-21相对表达量比较差异无统计学意义(P均0.05),在年龄大于中位年龄、存在淋巴结转移、分化程度低及已浸透浆膜层患者的癌组织组中miR-21相对表达量高于年龄小于中位年龄、无淋巴结转移、分化程度高及未浸透浆膜层患者的癌组织(P均0.05)。结论miR-21在大肠癌石蜡包埋组织中表达升高,其可能参与肿瘤的发生发展、分化及转移等生物学行为的调节;石蜡包埋组织可能成为研究肿瘤微小RNA的一种重要资源。
[Abstract]:Objective to observe the expression of small RNA-21 (miR-21) in paraffin embedded tissues of colorectal cancer and to explore its clinical significance. Methods RNA, was extracted from paraffin embedded cancer tissues of 40 patients with colorectal cancer and normal mucosa tissues outside 5 cm adjacent to carcinoma. Reverse transcription real-time fluorescence quantitative PCR was used to detect miR-21, and analyze the expression of miR-21 in cancer tissues and the presence of colorectal cancer patients. The relationship between the pathological features of the bed. Results the relative expression of miR-21 in cancer tissues was -4.28 卤1.67, and that in adjacent normal tissues was -2.05 卤1.85 miR-21, which was higher than that in adjacent normal mucosa tissues (P0.05). There was no significant difference in the relative expression of miR-21 in cancer tissues of patients with different sex, tumor morphology and location of tumor (all P 0. 05), and lymph node metastasis was found in patients with age greater than the median age (P < 0. 05). The relative expression of miR-21 in the patients with low differentiation and infiltrated serous layer was higher than that in the patients with age less than median age, no lymph node metastasis, high differentiation degree and without serous layer infiltration (P0. 05). Conclusion the expression of miR-21 in paraffin embedded tissues of colorectal cancer is increased, which may be involved in the regulation of biological behavior, such as tumorigenesis, differentiation and metastasis. Paraffin embedded tissue may be an important resource for the study of small RNA in colorectal cancer.
【作者单位】：承德医学院;
【基金】：河北省高校重点学科建设资助项目(031412) 河北省教育厅重点课题资助项目(ZD2014013) 河北省研究生创新资助项目(2016-275)
【分类号】：R735.34

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