爆米花型金纳米用于信号放大构建夹心型免疫传感器测定甲胎蛋白

发布时间：2018-10-08 16:39

【摘要】：通过原位合成法以金纳米种子作为母核诱导纳米金在其表面原位生长生成爆米花型金纳米粒子,标记甲胎蛋白抗体(anti-AFP)和辣根过氧化物酶(HRP)结合形成免疫复合物(antiAFP@popcorn-shaped GNP@HRP)作为检测抗体。氧化石墨烯-亚甲基蓝-金纳米粒子(GOM B-AuNPs)纳米复合材料滴涂到玻碳电极(GCE)表面,形成纳米复合物用于捕获甲胎蛋白抗体构建了免疫传感平台(anti-AFP/GO-MB-AuNPs/GCE)。实验采用夹心型免疫分析模式,功能化爆米花型金纳米材料与石墨烯纳米复合物呈现出良好的性能,建立了一种可行的电流型免疫分析法用于灵敏分析血清样品中甲胎蛋白AFP。在优化的实验条件下,免疫传感器DPV电流响应与AFP浓度的对数值呈正比,线性范围为0.001~20 ng/mL,检测限为0.31 pg/mL(S/N=3)。制备的免疫传感器有良好的精密度,选择性和稳定性,可初步应用于临床检验中AFP测定。
[Abstract]:By in situ synthesis, gold nanoparticles were induced to grow on the surface of gold nanoparticles by using gold nanoparticles as mother nucleus, and popcorn gold nanoparticles were formed by in situ synthesis. A fetoprotein antibody (anti-AFP) combined with horseradish peroxidase (HRP) to form an immune complex (antiAFP@popcorn-shaped GNP@HRP) was used to detect antibodies. Graphene oxide methylene blue gold nanoparticles (GOM B-AuNPs) nanoparticles were coated on the surface of glassy carbon electrode (GCE) to form nanocomposites to capture alpha-fetoprotein antibodies and build an immunosensor platform (anti-AFP/GO-MB-AuNPs/GCE). The sandwich immunoassay model was used in the experiment. The functionalized popcorn gold nanomaterials and graphene nanocomposites showed good properties. A feasible amperometric immunoassay was established for the sensitive analysis of alpha-fetoprotein AFP. in serum samples. Under the optimized experimental conditions, the current response of the immunosensor DPV is proportional to the logarithmic value of the concentration of AFP. The linear range is 0.001 ~ 20 ng/mL, and the detection limit is 0.31 pg/mL (S/N=3). The prepared immunosensor has good precision, selectivity and stability, and can be applied to clinical test for the determination of AFP.
【作者单位】：湖北民族学院化学与环境工程学院;湖北民族学院生物资源保护与利用湖北省重点实验室;
【基金】：国家自然科学基金项目(21665009,21361009) 湖北民族学院博士科研启动研究项目(MY2014B014)资助
【分类号】：O657.1;R730.43

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