宁夏地区鼻咽癌与EBV和HPV的相关性研究
发布时间：2018-10-09 19:37
【摘要】：目的:研究宁夏鼻咽癌患者组织中EB病毒(Epstein-Barr virus,EBV)与人乳头状瘤病毒(Human Papillomavirus,HPV)的感染情况及HPV基因型分布情况,并与南方鼻咽癌患者作比较,为探讨我区鼻咽癌预防、筛查、早期诊断和治疗、预后评价等提供科学依据。方法:收集2011年~2016年在宁夏医科大学附属总医院和上海公利医院经活检病理检查确诊的107例鼻咽癌患者石蜡组织样本采用核酸分子快速导流杂交基因芯片分型技术(HybriMax)和实时荧光定量PCR技术进行EBV定量检测和37种HPV亚型检测,并以95例鼻咽部黏膜组织慢性炎症患者的石蜡组织为对照组。结果:宁夏地区鼻咽癌患者与鼻咽部黏膜组织慢性炎症患者组织中EBV的总感染率分别是96.22%(51/53)、34.09%(15/44)上海地区鼻咽癌患者与鼻咽部黏膜组织慢性炎症患者组织中EBV的总感染率分别是85.2%(46/54)、17.6%(9/51),EBV感染在两地区鼻咽癌和鼻咽部黏膜组织慢性炎症患者组织中的表达差异有统计学意义(P0.05),EBV感染在宁夏与上海鼻咽癌患者组织中的表达差异无统计学意义(P0.05)。宁夏地区鼻咽癌患者与鼻咽部黏膜组织慢性炎症患者组织中HPV总感染性率为分别是45.2%(24/53)、9%(4/44)HPV感染总阳性率在鼻咽癌和鼻咽部黏膜组织慢性炎症组织中的表达差异有统计学意义(P0.05),其中单一类型感染最常见的为HPV16+45.83%(11/24),其次为HPV71+9.16%(7/24),EBV和HPV两种病毒混合感染在宁夏鼻咽癌和鼻咽部黏膜组织慢性炎症患者组织中总感染率分别为45.3%(24/53)和4.5%(2/44),HPV与EBV两种病毒混合感染在宁夏鼻咽癌和鼻咽部黏膜组织慢性炎症患者组织中的表达差异有统计学意义(P0.05)宁夏地区鼻咽癌患者组织中EBV感染与年龄、性别、民族、病理类型、临床分期无明显相关性,差异无统计学意义(P0.05)。结论:宁夏地区鼻咽癌发生发展与EBV和HPV感染密切相关,最主要以EBV感染为主,有一定数量的HPV感染,HPV感染亚型中以单一感染HPV16+型最常见,同时有大约50%的鼻咽癌患者存在EBV和HPV混合感染。宁夏地区鼻咽癌患者组织中EBV感染与年龄、性别、民族、病理类型、临床分期无明显相关性。上海地区鼻咽癌发生、发展主要与EBV感染相关,有极少量的HPV感染,EBV感染在宁夏地区和上海地区鼻咽癌患者组织中无明显差异
[Abstract]:Objective: to study the infection of EB virus (Epstein-Barr virus,EBV) and human papillomavirus (Human Papillomavirus,HPV) and the distribution of HPV genotypes in patients with nasopharyngeal carcinoma (NPC) in Ningxia. Early diagnosis and treatment, prognosis evaluation and so on provides the scientific basis. Methods: from 2011 to 2016, 107 paraffin tissue samples of nasopharyngeal carcinoma confirmed by biopsy and pathological examination were collected from affiliated General Hospital of Ningxia Medical University and Shanghai Gongli Hospital. (HybriMax) and real-time fluorescence quantitative PCR were used to detect EBV and 37 HPV subtypes. The paraffin tissues of 95 patients with chronic inflammation of nasopharyngeal mucosa were taken as control group. Results: the total infection rate of EBV was 96.22% (51 / 53) and 34.09% (15 / 44) in patients with nasopharyngeal carcinoma and chronic inflammation of nasopharyngeal mucosa, respectively. The total infection rate of EBV in patients with nasopharyngeal carcinoma and patients with chronic inflammation of nasopharyngeal mucosa in Shanghai was 96.22% (51 / 53) and 34.09% (15 / 44) respectively. The rates of EBV infection were 85.2% (46 / 54) and 17.6% (9 / 51) respectively. There was significant difference in the expression of EBV infection in patients with chronic inflammation of nasopharyngeal carcinoma and nasopharyngeal mucosa in the two regions (P0.05). There was no significant difference in the expression of EBV infection between Ningxia and Shanghai (P0.05). The total infection rate of HPV was 45.2% (24 / 53) or 9% (4 / 44) in patients with nasopharyngeal carcinoma and chronic inflammation of nasopharyngeal mucosa in Ningxia area. The most common type of infection was HPV16 45.83% (11 / 24), followed by HPV71 9.16% (7 / 24). The total infection rates of HPV71 and HPV were 45.3% (24 / 53) and 4.5% (2 / 44) in patients with chronic inflammation of nasopharyngeal mucosa in Ningxia. There was significant difference in the expression of EBV between nasopharyngeal carcinoma and nasopharyngeal mucosa in patients with chronic inflammation of nasopharyngeal carcinoma (P0.05). Gender, nationality, pathological type, clinical stage had no significant correlation, the difference was not statistically significant (P0.05). Conclusion: the occurrence and development of nasopharyngeal carcinoma in Ningxia area are closely related to EBV and HPV infection, the main infection is EBV infection, and the single infection HPV16 is the most common subtype of HPV infection. At the same time, about 50% of NPC patients have mixed infection of EBV and HPV. There was no significant correlation between EBV infection and age, sex, nationality, pathological type and clinical stage in patients with nasopharyngeal carcinoma in Ningxia. The occurrence and development of nasopharyngeal carcinoma in Shanghai area is mainly related to EBV infection. There is no significant difference in HPV infection between Ningxia region and Shanghai area.
【学位授予单位】：宁夏医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R739.63
[Abstract]:Objective: to study the infection of EB virus (Epstein-Barr virus,EBV) and human papillomavirus (Human Papillomavirus,HPV) and the distribution of HPV genotypes in patients with nasopharyngeal carcinoma (NPC) in Ningxia. Early diagnosis and treatment, prognosis evaluation and so on provides the scientific basis. Methods: from 2011 to 2016, 107 paraffin tissue samples of nasopharyngeal carcinoma confirmed by biopsy and pathological examination were collected from affiliated General Hospital of Ningxia Medical University and Shanghai Gongli Hospital. (HybriMax) and real-time fluorescence quantitative PCR were used to detect EBV and 37 HPV subtypes. The paraffin tissues of 95 patients with chronic inflammation of nasopharyngeal mucosa were taken as control group. Results: the total infection rate of EBV was 96.22% (51 / 53) and 34.09% (15 / 44) in patients with nasopharyngeal carcinoma and chronic inflammation of nasopharyngeal mucosa, respectively. The total infection rate of EBV in patients with nasopharyngeal carcinoma and patients with chronic inflammation of nasopharyngeal mucosa in Shanghai was 96.22% (51 / 53) and 34.09% (15 / 44) respectively. The rates of EBV infection were 85.2% (46 / 54) and 17.6% (9 / 51) respectively. There was significant difference in the expression of EBV infection in patients with chronic inflammation of nasopharyngeal carcinoma and nasopharyngeal mucosa in the two regions (P0.05). There was no significant difference in the expression of EBV infection between Ningxia and Shanghai (P0.05). The total infection rate of HPV was 45.2% (24 / 53) or 9% (4 / 44) in patients with nasopharyngeal carcinoma and chronic inflammation of nasopharyngeal mucosa in Ningxia area. The most common type of infection was HPV16 45.83% (11 / 24), followed by HPV71 9.16% (7 / 24). The total infection rates of HPV71 and HPV were 45.3% (24 / 53) and 4.5% (2 / 44) in patients with chronic inflammation of nasopharyngeal mucosa in Ningxia. There was significant difference in the expression of EBV between nasopharyngeal carcinoma and nasopharyngeal mucosa in patients with chronic inflammation of nasopharyngeal carcinoma (P0.05). Gender, nationality, pathological type, clinical stage had no significant correlation, the difference was not statistically significant (P0.05). Conclusion: the occurrence and development of nasopharyngeal carcinoma in Ningxia area are closely related to EBV and HPV infection, the main infection is EBV infection, and the single infection HPV16 is the most common subtype of HPV infection. At the same time, about 50% of NPC patients have mixed infection of EBV and HPV. There was no significant correlation between EBV infection and age, sex, nationality, pathological type and clinical stage in patients with nasopharyngeal carcinoma in Ningxia. The occurrence and development of nasopharyngeal carcinoma in Shanghai area is mainly related to EBV infection. There is no significant difference in HPV infection between Ningxia region and Shanghai area.
【学位授予单位】：宁夏医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R739.63
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