黄腐酚对顺铂诱导H1650细胞凋亡的影响
发布时间：2018-10-18 10:36
【摘要】：目的以顺铂为主的非小细胞肺癌化疗方案不断更新,目前研究旨在寻求疗效的放大及毒副作用的缩小。黄腐酚被证明有抗癌及保护正常细胞的作用。本实验主要探讨黄腐酚的不同给药方式对顺铂诱导人非小细胞肺癌细胞株H1650凋亡的影响。方法采用CCK-8法检测黄腐酚、顺铂单用及先用低浓度黄腐酚干预,后补入顺铂对H1650细胞增殖的影响;使用流式细胞术分别分析先用黄腐酚后用顺铂、先用顺铂再补入黄腐酚2种干预方式对H1650细胞凋亡率或存活率的影响。结果 CCK-8检测结果显示,2μmol/L黄腐酚干预H1650细胞48h后,继续给予20、40和80μmol/L顺铂干预24h,抑制细胞增殖率分别为(13.16±1.06)%、(19.14±1.67)%和(28.17±2.79)%,均低于同浓度顺铂单纯干预24h组的(18.96±3.02)%、(23.28±1.43)%和(39.79±3.44)%,P0.05。流式细胞术检测结果显示,10、20和40μmol/L顺铂干预细胞24h后,继续给予7[(85.66±3.19)%、(74.62±2.98)%和(59.55±2.70)%]或14μmol/L[(82.04±2.88)%、(76.50±3.11)%和(61.76±3.01)%]黄腐酚干预24h,其细胞存活率明显高于同浓度顺铂单纯干预48h组的(72.60±2.69)%、(56.98±3.80)%和(44.15±2.01)%,P0.05。3μmol/L黄腐酚干预细胞24h,弃培养液,更换10、20和40μmol/L顺铂再干预24h,其细胞凋亡率分别为(29.79±3.78)%、(30.53±3.89)%和(39.48±4.98)%,明显高于顺铂单纯干预24h组的(17.21±3.01)%、(14.53±2.99)%和(18.88±4.20)%,P0.05。结论黄腐酚直接有效保护顺铂对H1650细胞的杀伤,但低浓度黄腐酚的前期干预间接提高了顺铂对H1650细胞的凋亡率。
[Abstract]:Objective the chemotherapy regimen of cisplatin-based non-small cell lung cancer (NSCLC) has been continuously updated. Xanthohumol has been shown to have anti-cancer and protective effects on normal cells. This study was designed to investigate the effects of different administration of xanthate on apoptosis of human non-small cell lung cancer cell line H1650 induced by cisplatin. Methods CCK-8 assay was used to detect the effects of xanthohumol, cisplatin alone and low concentration xanthate on the proliferation of H1650 cells, and flow cytometry was used to analyze the proliferation of H1650 cells. Effects of cisplatin and xanthate on apoptosis and survival of H1650 cells. Results the results of CCK-8 assay showed that the inhibition rate of proliferation of H1650 cells was (13.16 卤1.06)%, (19.14 卤1.67)% and (28.17 卤2.79)%, respectively, which was significantly lower than that of the control group (18.96 卤3.02)%, (23.28 卤1.43)% and (39.79 卤3.44)%, respectively, after 48 hours of treatment with 2 渭 mol/L xanthohumol and 80 渭 mol/L cisplatin for 24 h, respectively, P 0.055.The results showed that the inhibition rate was (18.96 卤3.02)%, (23.28 卤1.43)% and (39.79 卤3.44)%, respectively. The results of flow cytometry showed that 10 渭 mol/L and 40 渭 mol/L cisplatin treated the cells for 24 h. After continuous administration of 7 [(85.66 卤3.19)%, (74.62 卤2.98)% and (59.55 卤2.70)%] or 14 渭 mol/L [(82.04 卤2.88)%, (76.50 卤3.11)% and (61.76 卤3.01)%] xanthate for 24 h, the cell survival rate was significantly higher than that in the 48 h group treated with cisplatin alone (72.60 卤2.69)%, (56.98 卤3.80)% and (44.15 卤2.01)%, P0.05.3 渭 mol/L xanthol for 24 h. The cell apoptosis rates were (29.79 卤3.78)%, (30.53 卤3.89)% and (39.48 卤4.98)% in 10 渭 mol/L and 40 渭 mol/L cisplatin replacement group for 24 h, respectively, which were significantly higher than those in cisplatin alone group (17.21 卤3.01)%, (14.53 卤2.99)% and (18.88 卤4.20)%, P0.05%. Conclusion xanthate can directly protect H1650 cells against cisplatin, but the early intervention of low concentration xanthohumol can indirectly increase the apoptosis rate of Cisplatin on H1650 cells.
【作者单位】： 武威职业学院直属附属医院老年病科;甘肃省医学科学研究院·甘肃省肿瘤医院转化医学研究中心;长春工业大学化学与生命科学学院;
【基金】：甘肃省中医药管理局科研课题(GKZ-2015-9)
【分类号】：R734.2
本文编号：2278867
[Abstract]:Objective the chemotherapy regimen of cisplatin-based non-small cell lung cancer (NSCLC) has been continuously updated. Xanthohumol has been shown to have anti-cancer and protective effects on normal cells. This study was designed to investigate the effects of different administration of xanthate on apoptosis of human non-small cell lung cancer cell line H1650 induced by cisplatin. Methods CCK-8 assay was used to detect the effects of xanthohumol, cisplatin alone and low concentration xanthate on the proliferation of H1650 cells, and flow cytometry was used to analyze the proliferation of H1650 cells. Effects of cisplatin and xanthate on apoptosis and survival of H1650 cells. Results the results of CCK-8 assay showed that the inhibition rate of proliferation of H1650 cells was (13.16 卤1.06)%, (19.14 卤1.67)% and (28.17 卤2.79)%, respectively, which was significantly lower than that of the control group (18.96 卤3.02)%, (23.28 卤1.43)% and (39.79 卤3.44)%, respectively, after 48 hours of treatment with 2 渭 mol/L xanthohumol and 80 渭 mol/L cisplatin for 24 h, respectively, P 0.055.The results showed that the inhibition rate was (18.96 卤3.02)%, (23.28 卤1.43)% and (39.79 卤3.44)%, respectively. The results of flow cytometry showed that 10 渭 mol/L and 40 渭 mol/L cisplatin treated the cells for 24 h. After continuous administration of 7 [(85.66 卤3.19)%, (74.62 卤2.98)% and (59.55 卤2.70)%] or 14 渭 mol/L [(82.04 卤2.88)%, (76.50 卤3.11)% and (61.76 卤3.01)%] xanthate for 24 h, the cell survival rate was significantly higher than that in the 48 h group treated with cisplatin alone (72.60 卤2.69)%, (56.98 卤3.80)% and (44.15 卤2.01)%, P0.05.3 渭 mol/L xanthol for 24 h. The cell apoptosis rates were (29.79 卤3.78)%, (30.53 卤3.89)% and (39.48 卤4.98)% in 10 渭 mol/L and 40 渭 mol/L cisplatin replacement group for 24 h, respectively, which were significantly higher than those in cisplatin alone group (17.21 卤3.01)%, (14.53 卤2.99)% and (18.88 卤4.20)%, P0.05%. Conclusion xanthate can directly protect H1650 cells against cisplatin, but the early intervention of low concentration xanthohumol can indirectly increase the apoptosis rate of Cisplatin on H1650 cells.
【作者单位】： 武威职业学院直属附属医院老年病科;甘肃省医学科学研究院·甘肃省肿瘤医院转化医学研究中心;长春工业大学化学与生命科学学院;
【基金】：甘肃省中医药管理局科研课题(GKZ-2015-9)
【分类号】：R734.2
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