嵌合分子靶向降解鼻咽癌细胞表皮生长因子受体的实验研究

发布时间：2018-10-19 11:24

【摘要】：目的:检测合成的蛋白靶向嵌合分子(EGF-PROTAC)通过泛素-蛋白酶体途径体外对人鼻咽癌CNE-2细胞表皮生长因子受体(EGFR)靶向降解作用,并证实对该细胞增殖、凋亡的影响。方法:EGF-PROTAC处理细胞后,应用Western blot技术分析EGFR蛋白表达水平;CKK-8、流式细胞术、Transwell侵袭实验检测EGF-PROTAC对CNE-2产生的生物学效应。结果:Western blot技术显示EGF-PROTAC组EGFR蛋白的表达水平显著低于对照组(P0.05);CKK-8法显示EGF-PROTAC组3、6、9、12hCNE-2细胞存活率显著低于溶剂对照组(P0.05);流式细胞术显示EGF-PROTAC组细胞凋亡指数显著高于对照组(P0.05);Transwell法显示EGF-PROTAC组CNE-2细胞侵袭到Transwell小室滤膜下细胞数显著低于溶剂对照组(P0.05)。结论:EGF-PROTAC能够靶向降解CNE-2细胞的EGFR,在体外能抑制鼻咽癌细胞的生长、促进凋亡。
[Abstract]:Aim: to investigate the effect of synthesized protein targeted chimeric molecules (EGF-PROTAC) on the degradation of epidermal growth factor receptor (EGFR) by ubiquitin proteasome pathway in human nasopharyngeal carcinoma (NPC) CNE-2 cells in vitro, and to confirm the effects on the proliferation and apoptosis of human nasopharyngeal carcinoma (NPC) CNE-2 cells. Methods: after treated with EGF-PROTAC, the expression of EGFR protein was analyzed by Western blot, and the biological effect of EGF-PROTAC on CNE-2 was detected by CKK-8, flow cytometry and Transwell invasion assay. Results: Western blot technique showed that the expression of EGFR protein in EGF-PROTAC group was significantly lower than that in control group (P0.05), the survival rate of CNE-2 cells in EGF-PROTAC group was significantly lower than that in solvent control group (P0.05), and the apoptotic index in EGF-PROTAC group was significantly higher than that in control group (P0.05); Transwell). The results showed that the number of CNE-2 cells in EGF-PROTAC group was significantly lower than that in control group (P0.05). Conclusion: EGFR, which can target CNE-2 cells, can inhibit the growth of nasopharyngeal carcinoma cells and promote apoptosis in vitro.
【作者单位】：武汉大学中南医院耳鼻咽喉头颈外科;
【分类号】：R739.63

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