FAK、NF-κBp65及VEGF-C在非小细胞肺癌中的表达及临床意义

发布时间：2018-10-22 12:40

【摘要】：目的通过研究FAK、NF-κBp65及VEGF-C在非小细胞肺癌(non-small cell lung cancer,NSCLC)组织与癌旁正常组织及良性病变肺组织中的表达,探讨三者的表达与NSCLC临床病理特征及其与诊断和预后的关系。方法收集2012年12月至2013年12月解放军第四零一医院胸外科手术切除的非小细胞肺癌组织石蜡标本75例(标本要求:1.术前未做过放化疗;2.无其他器官原发性肿瘤;3.组织块石蜡包埋充分)。随机选择30例NSCLC癌旁正常组织和良性病变肺组织作为对照。入选标本的组织学类型、分化程度及临床分期等均经病理科医师确诊。采用免疫组织化学Envision二步法检测FAK、NF-κBp65及VEGF-C在75例NSCLC及癌旁组织标本中的表达。采用SPSS17.0统计软件进行分析,FAK、NF-κBp65及VEGF-C在NSCLC组织中的表达与患者临床病例特征之间的关系,实验组与对照组之间的比较采用χ2检验,三个免疫指标之间的相关性采用Spearman等级相关分析,以P0.05为差异有统计学意义。结果(1)FAK、VEGF-C的表达主要位于胞浆,染色阳性为胞浆中出现棕黄色颗粒;NF-κBp65的表达主要位于胞核和胞浆,染色阳性表达部位出现棕黄色颗粒。(2)肺癌组织中FAK、NF-κBp65及VEGF-C的阳性表达率分别为57.3%(43/75)、85.3%(64/75)、65.3%(49/75),而癌旁组织中三者的阳性率分别是13.3%(4/30)、16.7(5/30)、0%(0/30),显著低于其在肺癌组织中的表达,差异有统计学意义(P0.05)。(3)癌组织中三者的阳性率与癌细胞的分化程度、肿瘤分期及转移关系密切(P0.05)。(4)FAK与NF-κBp65,NF-κBp65与VEGF-C在肺癌组织中的表达分别成正相关关系(P0.05)。结论(1)FAK、NF-κBp65及VEGF-C在肺癌组织中的表达水平显著高于癌旁正常组织及良性病变中的表达,表明三者参与了肺癌的发生。(2)在肺癌组织中,FAK、NF-κBp65及VEGF-C的表达与肿瘤的分化程度、TNM分期相关,且肺癌的恶性程度越高,三者的表达水平越高,提示三者的表达水平越高,肺癌的预后越差。(3)FAK与NF-κBp65,NF-κBp65与VEGF-C在肺癌组织中的表达分别成正相关,但三者在肺癌发生发展中的相互作用机制有待进一步研究。
[Abstract]:Objective to investigate the expression of FAK,NF- 魏 Bp65 and VEGF-C in non-small cell lung cancer (non-small cell lung cancer,NSCLC), adjacent normal tissues and benign lung tissues, and to explore the relationship between the expression of NSCLC and clinicopathological features, diagnosis and prognosis. Methods from December 2012 to December 2013, 75 cases of non-small cell lung cancer (NSCLC) tissues were collected from thoracic surgery of the first Hospital of PLA from December 2012 to December 2013 (specimen requirement: 1. No chemoradiotherapy before operation; 2. No other organ primary tumor; 3. Tissue mass is well embedded in paraffin). Thirty cases of NSCLC adjacent normal tissues and benign lung tissues were randomly selected as control group. The histological types, differentiation and clinical stages of the selected specimens were confirmed by pathologists. The expression of FAK,NF- 魏 Bp65 and VEGF-C in 75 cases of NSCLC and adjacent tissues was detected by immunohistochemical Envision two-step method. The relationship between the expression of FAK,NF- 魏 Bp65 and VEGF-C in NSCLC tissues and the clinical characteristics of patients was analyzed by SPSS17.0 statistical software. The comparison between the experimental group and the control group was carried out by 蠂 2 test. The correlation between the three immune indexes was analyzed by Spearman grade correlation analysis. P0.05 as the difference was statistically significant. Results (1) the expression of FAK,VEGF-C was mainly located in the cytoplasm, and the expression of NF- 魏 Bp65 was mainly located in the nucleus and cytoplasm. The positive expression rates of FAK,NF- 魏 Bp65 and VEGF-C in lung cancer tissues were 57.3% (43 / 75), 85.3% (64 / 75), 65.3% (49 / 75), respectively. The positive rates of FAK,NF- 魏 Bp65 and VEGF-C in adjacent tissues were 13.3% (4 / 30), 16.7 (5 / 30), 0% (0 / 30), respectively, which were significantly lower than those in lung cancer tissues. The difference was statistically significant (P0.05). (3). The positive rates of the three cells were closely related to the degree of differentiation, tumor staging and metastasis (P0.05). (4). The expression of FAK, NF- 魏 Bp65,NF- 魏 Bp65 and VEGF-C were positively correlated in lung cancer tissues (P0.05). Conclusion (1) the expression levels of FAK,NF- 魏 Bp65 and VEGF-C in lung cancer tissues are significantly higher than those in adjacent normal tissues and benign lesions, indicating that the expression of FAK,NF- 魏 Bp65 and VEGF-C in lung cancer tissues is related to the degree of differentiation and TNM stage. The higher the malignant degree of lung cancer is, the higher the expression level of the three is, which indicates that the higher the expression level of the three is, the worse the prognosis of lung cancer is. (3) the expression of FAK and NF- 魏 Bp65,NF- 魏 Bp65 and VEGF-C are positively correlated in lung cancer. However, the interaction mechanism of these three factors in the development of lung cancer needs to be further studied.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R734.2

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