TRAF6表达与胃癌患者营养状况及预后的关系
发布时间：2018-10-22 15:43
【摘要】：目的目前有关肿瘤坏死因子受体相关因子6(tumor necrosis receptor-associated factor 6,TRAF6)在胃癌相关领域的研究仍有限。本研究通过检测胃癌患者组织中TRAF6表达,探讨其与患者营养状况和预后的关系,为胃癌治疗提供新思路。方法采用逆转录-聚合酶链反应、蛋白质印迹法、酶联免疫吸附试验检测2008-01-01-2013-06-30浙江省人民医院102例胃癌患者及53例腹腔良性疾病患者组织(腹直肌、血清)中TRAF6mRNA和蛋白的表达情况,并结合临床资料分析其对胃癌患者营养状况及预后的影响。结果胃癌患者腹直肌中TRAF6 mRNA的相对表达量(3.89±0.09)较腹腔良性疾病患者(1.77±1.08)明显升高,t=13.050,P0.001。蛋白质印迹法结果显示,胃癌患者腹直肌中TRAF6蛋白表达较腹腔良性疾病患者明显升高,P0.05。胃癌患者血清中TRAF6蛋白的相对表达量(4.89±1.09)较腹腔良性疾病患者(2.78±1.18)明显升高,t=14.595,P0.001。胃癌患者血清中TRAF6蛋白表达水平与患者体质量丢失(χ~2=12.901,P0.001)、血清白蛋白(χ~2=17.422,P0.001)及血红蛋白水平(χ~2=4.886,P=0.027)有关。胃癌患者血清中TRAF6蛋白表达水平与患者胃癌病理类型(χ~2=6.154,P=0.046)、淋巴结转移(χ~2=10.237,P=0.001)及TNM分期(χ~2=7.727,P=0.005)有关。胃癌患者血清中TRAF6蛋白表达水平与患者生存率有关,χ~2=6.641,P=0.010。结论胃癌患者腹直肌及血清中TRAF6mRNA和蛋白表达升高,且血清中TRAF6蛋白表达水平患者的营养状况及预后存在关联。TRAF6可能与胃癌患者癌性恶病质的发生发展有关。
[Abstract]:Objective at present, the research on tumor necrosis factor receptor related factor 6 (tumor necrosis receptor-associated factor 6 TRAF 6 is still limited in the field of gastric cancer. The aim of this study was to investigate the relationship between TRAF6 expression and nutritional status and prognosis of patients with gastric cancer, and to provide a new approach for the treatment of gastric cancer. Methods 102 patients with gastric cancer and 53 patients with benign abdominal diseases (rectus abdominis) were detected by reverse transcription-polymerase chain reaction (RT-PCR), Western blot and enzyme linked immunosorbent assay (Elisa). The expression of TRAF6mRNA and protein in serum and its effect on nutritional status and prognosis of patients with gastric cancer were analyzed with clinical data. Results the relative expression of TRAF6 mRNA in rectus abdominis in gastric cancer patients (3.89 卤0.09) was significantly higher than that in benign abdominal diseases (1.77 卤1.08). The results of Western blotting showed that the expression of TRAF6 protein in rectus abdominis of gastric cancer patients was significantly higher than that in patients with benign abdominal diseases (P0.05. The relative expression of TRAF6 protein in serum of patients with gastric cancer (4.89 卤1.09) was significantly higher than that of patients with benign abdominal diseases (2.78 卤1.18). The expression of TRAF6 protein in serum of patients with gastric cancer was correlated with the loss of body mass (蠂 ~ 2 ~ 2 ~ (12.901) P ~ (0.001), serum albumin (蠂 ~ (2) ~ (2) ~ (17.422) P ~ (0.001) and hemoglobin (蠂 ~ (2) ~ (4.886) P ~ (0.027). The expression of TRAF6 protein in serum of patients with gastric cancer was correlated with the pathological type of gastric cancer (蠂 ~ 26.154), lymph node metastasis (蠂 ~ 2 + 10.237) and TNM stage (蠂 ~ 27.727 P ~ (0.005). The expression of TRAF6 protein in serum of patients with gastric cancer was correlated with survival rate (蠂 ~ 2 ~ 2 ~ (6.641). Conclusion the expression of TRAF6mRNA and protein in rectus abdominis and serum of gastric cancer patients is increased, and the nutritional status and prognosis of patients with serum TRAF6 protein expression are related. TRAF6 may be related to the occurrence and development of cancer cachexia in gastric cancer patients.
【作者单位】： 绍兴市中心医院普外科;浙江省立同德医院普外科;
【基金】：浙江省科技厅计划(2013C33203)
【分类号】：R735.2
[Abstract]:Objective at present, the research on tumor necrosis factor receptor related factor 6 (tumor necrosis receptor-associated factor 6 TRAF 6 is still limited in the field of gastric cancer. The aim of this study was to investigate the relationship between TRAF6 expression and nutritional status and prognosis of patients with gastric cancer, and to provide a new approach for the treatment of gastric cancer. Methods 102 patients with gastric cancer and 53 patients with benign abdominal diseases (rectus abdominis) were detected by reverse transcription-polymerase chain reaction (RT-PCR), Western blot and enzyme linked immunosorbent assay (Elisa). The expression of TRAF6mRNA and protein in serum and its effect on nutritional status and prognosis of patients with gastric cancer were analyzed with clinical data. Results the relative expression of TRAF6 mRNA in rectus abdominis in gastric cancer patients (3.89 卤0.09) was significantly higher than that in benign abdominal diseases (1.77 卤1.08). The results of Western blotting showed that the expression of TRAF6 protein in rectus abdominis of gastric cancer patients was significantly higher than that in patients with benign abdominal diseases (P0.05. The relative expression of TRAF6 protein in serum of patients with gastric cancer (4.89 卤1.09) was significantly higher than that of patients with benign abdominal diseases (2.78 卤1.18). The expression of TRAF6 protein in serum of patients with gastric cancer was correlated with the loss of body mass (蠂 ~ 2 ~ 2 ~ (12.901) P ~ (0.001), serum albumin (蠂 ~ (2) ~ (2) ~ (17.422) P ~ (0.001) and hemoglobin (蠂 ~ (2) ~ (4.886) P ~ (0.027). The expression of TRAF6 protein in serum of patients with gastric cancer was correlated with the pathological type of gastric cancer (蠂 ~ 26.154), lymph node metastasis (蠂 ~ 2 + 10.237) and TNM stage (蠂 ~ 27.727 P ~ (0.005). The expression of TRAF6 protein in serum of patients with gastric cancer was correlated with survival rate (蠂 ~ 2 ~ 2 ~ (6.641). Conclusion the expression of TRAF6mRNA and protein in rectus abdominis and serum of gastric cancer patients is increased, and the nutritional status and prognosis of patients with serum TRAF6 protein expression are related. TRAF6 may be related to the occurrence and development of cancer cachexia in gastric cancer patients.
【作者单位】： 绍兴市中心医院普外科;浙江省立同德医院普外科;
【基金】：浙江省科技厅计划(2013C33203)
【分类号】：R735.2
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