CRT和BAP31在非小细胞肺癌中的表达及临床意义
发布时间：2018-10-31 07:25
【摘要】：目的:通过检测钙网蛋白(calreticulin,CRT)和B细胞受体相关蛋白31(B-cell receptor-associated protein 31,BAP31)在非小细胞肺癌组织、癌旁组织以及正常肺组织中的表达情况,并分析两者在非小细胞肺癌患者中与年龄、性别、吸烟、病理类型、淋巴结转移以及临床分期的关系,进而探索CRT和BAP31在非小细胞肺癌发生、发展、浸润以及转移过程中所起的作用,同时,通过对二者在非小细胞肺癌中表达相关性的分析,探讨二者在非小细胞肺癌的发生、发展过程中是否具有协同或拮抗作用。方法:选取西南医科大学附属第一医院2015年1月至2015年12月胸外科手术切除并且术后均经病理诊断明确为非小细胞肺癌的标本60例,其中选取鳞癌30例,腺癌30例,并选取相对应的癌旁组织(至少距肿瘤边缘5cm以上)30例(随机选取,其中包括鳞癌17例,腺癌13例)为检测对象,所选病例均具备完整的临床诊断和治疗资料,并且排除其他肺部疾病及严重的心、肝、肾和代谢性疾病,取得待测标本前患者均未进行放疗、化疗、分子靶向治疗及其他针对恶性肿瘤的治疗;另外选取正常肺组织30例作为对照组。通过免疫组化方法检测非小细胞肺癌组织、癌旁组织、正常肺组织中CRT和BAP31的表达情况,并分析两指标在非小细胞肺癌患者中的表达与年龄、性别、吸烟、病理分型、淋巴结转移、临床分期的关系,进一步分析两指标在非小细胞肺癌中的表达是否具有相关性。结果:1.CRT在非小细胞肺癌组织中表达的阳性率为88.33%,明显高于其在正常肺组织和癌旁肺组织中的阳性表达率(6.67%和13.33%),差异具有统计学意义(P0.01),但是正常肺组织和癌旁组织的表达差异不具有统计学意义(P0.05),CRT的阳性表达与非小细胞肺癌患者的性别、年龄、吸烟情况及病理分型无明显相关性,而与淋巴结转移和临床分期相关。2.BAP31在非小细胞肺癌组织中的阳性表达率为83.33%,明显高于其在正常肺组织和癌旁肺组织中的阳性表达率(3.33%和6.67%),差异具有统计学意义(P0.01),但是正常肺组织和癌旁组织的表达差异不具有统计学意义(P0.05),BAP31的阳性表达与患者的性别、年龄、吸烟情况、病理分型无明显相关性,其与淋巴结转移和不同TNM分期也无明显相关性。3.CRT与BAP31在非小细胞肺癌组织中的表达无明显相关性。结论:1.CRT在非小细胞肺癌中呈高表达,其阳性表达与淋巴结转移和分期相关,可能参与非小细胞肺癌的发生、发展过程。2.BAP31在非小细胞肺癌中呈高表达,其阳性表达与患者年龄、性别、病理分型、淋巴结转移和TNM分期无明显相关性。3.CRT与BAP31在非小细胞肺癌组织中的表达无明显相关性,需更大样本的研究加以明确证实。
[Abstract]:Objective: to investigate the expression of calmodulin (calreticulin,CRT) and B cell receptor-associated protein 31 (B-cell receptor-associated protein 31) in non-small cell lung cancer (NSCLC), paracancerous tissues and normal lung tissues. The relationship between age, sex, smoking, pathological type, lymph node metastasis and clinical stage in patients with non-small cell lung cancer (NSCLC) was analyzed to explore the role of CRT and BAP31 in the occurrence and development of NSCLC. The role of invasion and metastasis in the process of invasion and metastasis. At the same time, through the analysis of the correlation between the expression of both in non-small cell lung cancer, to explore whether they have synergistic or antagonistic action in the occurrence and development of non-small cell lung cancer. Methods: from January 2015 to December 2015, 60 cases of non-small cell lung cancer (NSCLC) were selected from the first affiliated Hospital of Southwest Medical University, including 30 cases of squamous cell carcinoma and 30 cases of adenocarcinoma. Thirty cases (including 17 cases of squamous cell carcinoma and 13 cases of adenocarcinoma) corresponding to adjacent tissues (at least more than 5cm from the edge of the tumor) were selected as subjects. All the selected cases had complete clinical diagnosis and treatment data. Other lung diseases and severe heart, liver, kidney and metabolic diseases were excluded. Patients were not treated with radiotherapy, chemotherapy, molecular targeted therapy and other treatment for malignant tumor before obtaining the specimen. In addition, 30 cases of normal lung tissue were selected as control group. The expression of CRT and BAP31 in non-small cell lung cancer (NSCLC) tissues, paracancerous tissues and normal lung tissues were detected by immunohistochemical method, and the expression and age, sex, smoking and pathological classification of the two markers in NSCLC patients were analyzed. The relationship between lymph node metastasis and clinical stage, and further analysis of the expression of the two markers in non-small cell lung cancer (NSCLC). Results: the positive rate of 1.CRT expression in non-small cell lung cancer tissues was 88.33, which was significantly higher than that in normal lung tissues and adjacent lung tissues (6.67% and 13.33%). The difference was statistically significant (P0.01), but there was no significant difference in the expression of), CRT between normal lung tissues and para-cancerous tissues (P0.05) and the sex and age of patients with non-small cell lung cancer (NSCLC). There was no significant correlation between smoking and pathological classification, but there was no correlation between smoking and lymph node metastasis and clinical stage. The positive expression rate of 2.BAP31 in non-small cell lung cancer was 83.33. The positive expression rate was significantly higher in normal lung tissues and adjacent lung tissues (3.33% vs 6.67%), and the difference was statistically significant (P0.01). However, there was no significant difference in the expression of BAP31 between normal lung tissues and paracancerous tissues (P0.05). There was no significant correlation between the positive expression of BAP31 and sex, age, smoking status, pathological type of patients. There was no significant correlation between the expression of 3.CRT and BAP31 in non-small cell lung cancer (NSCLC) and lymph node metastasis and different TNM stages. Conclusion: 1.CRT is highly expressed in non-small cell lung cancer, and its positive expression is related to lymph node metastasis and staging, which may be involved in the occurrence and development of non-small cell lung cancer. 2.BAP31 is highly expressed in non-small cell lung cancer. There was no significant correlation between the positive expression and age, sex, pathological type, lymph node metastasis and TNM staging. There was no significant correlation between the expression of 3.CRT and BAP31 in non-small cell lung cancer tissues, which needed to be confirmed by larger samples.
【学位授予单位】：西南医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R734.2
[Abstract]:Objective: to investigate the expression of calmodulin (calreticulin,CRT) and B cell receptor-associated protein 31 (B-cell receptor-associated protein 31) in non-small cell lung cancer (NSCLC), paracancerous tissues and normal lung tissues. The relationship between age, sex, smoking, pathological type, lymph node metastasis and clinical stage in patients with non-small cell lung cancer (NSCLC) was analyzed to explore the role of CRT and BAP31 in the occurrence and development of NSCLC. The role of invasion and metastasis in the process of invasion and metastasis. At the same time, through the analysis of the correlation between the expression of both in non-small cell lung cancer, to explore whether they have synergistic or antagonistic action in the occurrence and development of non-small cell lung cancer. Methods: from January 2015 to December 2015, 60 cases of non-small cell lung cancer (NSCLC) were selected from the first affiliated Hospital of Southwest Medical University, including 30 cases of squamous cell carcinoma and 30 cases of adenocarcinoma. Thirty cases (including 17 cases of squamous cell carcinoma and 13 cases of adenocarcinoma) corresponding to adjacent tissues (at least more than 5cm from the edge of the tumor) were selected as subjects. All the selected cases had complete clinical diagnosis and treatment data. Other lung diseases and severe heart, liver, kidney and metabolic diseases were excluded. Patients were not treated with radiotherapy, chemotherapy, molecular targeted therapy and other treatment for malignant tumor before obtaining the specimen. In addition, 30 cases of normal lung tissue were selected as control group. The expression of CRT and BAP31 in non-small cell lung cancer (NSCLC) tissues, paracancerous tissues and normal lung tissues were detected by immunohistochemical method, and the expression and age, sex, smoking and pathological classification of the two markers in NSCLC patients were analyzed. The relationship between lymph node metastasis and clinical stage, and further analysis of the expression of the two markers in non-small cell lung cancer (NSCLC). Results: the positive rate of 1.CRT expression in non-small cell lung cancer tissues was 88.33, which was significantly higher than that in normal lung tissues and adjacent lung tissues (6.67% and 13.33%). The difference was statistically significant (P0.01), but there was no significant difference in the expression of), CRT between normal lung tissues and para-cancerous tissues (P0.05) and the sex and age of patients with non-small cell lung cancer (NSCLC). There was no significant correlation between smoking and pathological classification, but there was no correlation between smoking and lymph node metastasis and clinical stage. The positive expression rate of 2.BAP31 in non-small cell lung cancer was 83.33. The positive expression rate was significantly higher in normal lung tissues and adjacent lung tissues (3.33% vs 6.67%), and the difference was statistically significant (P0.01). However, there was no significant difference in the expression of BAP31 between normal lung tissues and paracancerous tissues (P0.05). There was no significant correlation between the positive expression of BAP31 and sex, age, smoking status, pathological type of patients. There was no significant correlation between the expression of 3.CRT and BAP31 in non-small cell lung cancer (NSCLC) and lymph node metastasis and different TNM stages. Conclusion: 1.CRT is highly expressed in non-small cell lung cancer, and its positive expression is related to lymph node metastasis and staging, which may be involved in the occurrence and development of non-small cell lung cancer. 2.BAP31 is highly expressed in non-small cell lung cancer. There was no significant correlation between the positive expression and age, sex, pathological type, lymph node metastasis and TNM staging. There was no significant correlation between the expression of 3.CRT and BAP31 in non-small cell lung cancer tissues, which needed to be confirmed by larger samples.
【学位授予单位】：西南医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R734.2
【参考文献】
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1 施博文;乔文亮;韩玉栋;胡海洋;陈s，
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