大蒜素与化疗药物对食管癌细胞EC109疗效比较的实验研究
发布时间：2018-11-03 17:54
【摘要】：目的:通过与化疗药物比较的实验研究探讨大蒜素对食管癌的抗肿瘤效果及其可能机制。方法:不同浓度的大蒜素、5-氟尿嘧啶和顺铂作用于食管癌细胞EC109,分别在6 h、12 h、24 h、48 h和72 h应用CCK-8法检测EC109细胞的生长抑制率,并以分析细胞乳酸脱氢酶(LDH)活性反映各药物的细胞毒性;Annexin V/PI双染法检测空白对照组、Z-VAD-FMK(caspase-3活性抑制剂)组、大蒜素组、大蒜素+Z-VAD-FMK组、5-氟尿嘧啶组和顺铂组的细胞凋亡情况。分光光度测定法检测细胞内caspase-3、8、9的活性变化。结果:大蒜素呈浓度依赖性及时间依赖性抑制和杀伤食管癌细胞EC109;与5-氟尿嘧啶组和顺铂组比较,大蒜素组LDH活性明显下降;与对照组相比,大蒜素培养细胞内caspase-3、8的活性增强,而细胞内caspase-9的活性变化差异无统计学显著性。结论:大蒜素可能通过活化caspase-8激活外源性凋亡通路,诱导食管癌细胞EC109凋亡,从而抑制肿瘤细胞的生长,且与5-氟尿嘧啶和顺铂相比,大蒜素的细胞毒副作用明显减弱。
[Abstract]:Aim: to investigate the antitumor effect of allicin on esophageal carcinoma and its possible mechanism by comparing with chemotherapeutic drugs. Methods: different concentrations of allicin, 5-fluorouracil and cisplatin were used to detect the growth inhibition rate of EC109 cells in EC109, cells at 6 h, 12 h, 24 h, 48 h and 72 h, respectively. The activity of lactate dehydrogenase (LDH) was analyzed to reflect the cytotoxicity of various drugs. Annexin V/PI double staining method was used to detect apoptosis in control group, Z-VAD-FMK (caspase-3 activity inhibitor) group, allicin Z-VAD-FMK group, 5-fluorouracil group and cisplatin group. The activity of caspase-3,8,9 was detected by spectrophotometry. Results: compared with 5-fluorouracil group and cisplatin group, the LDH activity of allicin group decreased significantly compared with 5-fluorouracil group and cisplatin group. Compared with the control group, the activity of caspase-3,8 was increased in allicin culture cells, but there was no significant difference in the activity of caspase-9 in the cells. Conclusion: allicin may activate exogenous apoptosis pathway by activating caspase-8 and induce EC109 apoptosis in esophageal carcinoma cells, thus inhibiting the growth of tumor cells, and the cytotoxic side effects of allicin are significantly reduced compared with 5-fluorouracil and cisplatin.
【作者单位】： 福建医科大学附属龙岩第一医院心胸外科;福建医科大学附属龙岩第一医院重症医学科;
【基金】：福建省卫生厅青年科研基金资助项目(No.2013-2-150) 福建医科大学非直属附属医院科学研究专项基金资助项目(No.FZS13013Y)
【分类号】：R735.1
[Abstract]:Aim: to investigate the antitumor effect of allicin on esophageal carcinoma and its possible mechanism by comparing with chemotherapeutic drugs. Methods: different concentrations of allicin, 5-fluorouracil and cisplatin were used to detect the growth inhibition rate of EC109 cells in EC109, cells at 6 h, 12 h, 24 h, 48 h and 72 h, respectively. The activity of lactate dehydrogenase (LDH) was analyzed to reflect the cytotoxicity of various drugs. Annexin V/PI double staining method was used to detect apoptosis in control group, Z-VAD-FMK (caspase-3 activity inhibitor) group, allicin Z-VAD-FMK group, 5-fluorouracil group and cisplatin group. The activity of caspase-3,8,9 was detected by spectrophotometry. Results: compared with 5-fluorouracil group and cisplatin group, the LDH activity of allicin group decreased significantly compared with 5-fluorouracil group and cisplatin group. Compared with the control group, the activity of caspase-3,8 was increased in allicin culture cells, but there was no significant difference in the activity of caspase-9 in the cells. Conclusion: allicin may activate exogenous apoptosis pathway by activating caspase-8 and induce EC109 apoptosis in esophageal carcinoma cells, thus inhibiting the growth of tumor cells, and the cytotoxic side effects of allicin are significantly reduced compared with 5-fluorouracil and cisplatin.
【作者单位】： 福建医科大学附属龙岩第一医院心胸外科;福建医科大学附属龙岩第一医院重症医学科;
【基金】：福建省卫生厅青年科研基金资助项目(No.2013-2-150) 福建医科大学非直属附属医院科学研究专项基金资助项目(No.FZS13013Y)
【分类号】：R735.1
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