FTIR结合主成分分析法对直肠癌转移淋巴结的研究
发布时间：2018-11-08 21:20
【摘要】：应用傅里叶变换红外光谱(FTIR)联合主成分分析法(PCA),分析直肠癌转移淋巴结的谱学特征,并对直肠癌转移淋巴结和非转移淋巴结进行线性判别分析。80例直肠癌转移淋巴结和80例未转移淋巴结进行FTIR光谱分析,计算峰强并进行主成分分析,得出在波数4 000~1 700cm~(-1)范围主成分1(Principal components 1,PC1)是3 260cm~(-1),PC2为1 740cm~(-1)。波数1 700~1 000cm~(-1)范围,PC1为1 640cm~(-1),PC2为1 080cm~(-1),将良、恶性淋巴结光谱3 260,1 740,1 640,1 080cm~(-1)相对峰强比(I/I1 460)和波数1 080和1 300cm~(-1)进行t检验,良、恶性结果差异有统计学意义(p0.05),表明癌转移淋巴结中蛋白含量、蛋白的形成、氨基酸增多;脂肪含量明显减少与癌组织中无氧酵解脂肪含量减少有关。将相对峰强比(I1 080/I1 460,I1 640/I1 460,I3 260/I1 460,I1 740/I1 460,n=160)进行PCA聚类分析,结果显示可以将良恶性淋巴结鉴别,良性淋巴结聚类在第一和四象限,恶性淋巴结聚类在二和三象限。将相对峰强比、1 080和1 300cm~(-1)进行线性判别分析(LDA),将25例淋巴结作为验证集进行分析,得出PCA/LDA模型的敏感度是87.5%,特异度是88.5%。结果表明傅里叶变换红外光谱分析技术可成为术中原位、在体和快速诊断直肠癌淋巴结转移的一种简便方法。
[Abstract]:Fourier transform infrared spectroscopy (FTIR) and principal component analysis (PCA),) were used to analyze the spectral characteristics of metastatic lymph nodes in rectal cancer. The metastatic lymph nodes and non-metastatic lymph nodes of rectal cancer were analyzed by linear discriminant analysis. 80 cases of metastatic lymph nodes of rectal cancer and 80 cases of non-metastatic lymph nodes were analyzed by FTIR spectrum, the peak intensity was calculated and principal component analysis was performed. It is obtained that the principal component of 1 (Principal components 1p1 is 3 260 cm-1 and PC2 is 1 740 cm-1 in the range of 4 000 ~ (-1) cm ~ (-1). The main component is 1 (Principal components ~ (-1) and the PC2 is 1 740 cm ~ (-1) in the range of 4 000 ~ (-1) cm ~ (-1). The wave number is 1 700 ~ (-1) cm ~ (-1), PC1 is 1 640 cm ~ (-1) and PC2 is 1 080 cm ~ (-1). The relative peak / intensity ratio (I/I1 460) and wave numbers of 1 080 and 1 300 cm ~ (-1) of malignant lymph nodes were measured by t test. The difference between benign and malignant results was significant (p0. 05). The results showed that the protein content, protein formation and amino acid increased in metastatic lymph nodes. The decrease of fat content was related to the decrease of anaerobic glycolytic fat content in cancer tissue. The relative peak-to-intensity ratio (I1 080/I1 460G / I1 640/I1 460N / I3 260/I1 460C / I1 740/I1 460NNX 160) was analyzed by PCA cluster analysis. The results showed that benign and malignant lymph nodes could be distinguished from benign lymph nodes in the first and fourth quadrant, and benign lymph nodes could be clustered in the first and fourth quadrants. Malignant lymph nodes were clustered in two and three quadrants. The relative peak / intensity ratio, 1 080 cm ~ (-1) and 1 300 cm ~ (-1) were analyzed by linear discriminant analysis (LDA),). The results showed that the sensitivity and specificity of the PCA/LDA model were 87.5 and 88.5respectively. The results show that Fourier transform infrared spectroscopy (FTIR) can be used as a simple method for the diagnosis of lymph node metastasis of rectal cancer in situ and in vivo.
【作者单位】： 陕西省人民医院普外二科;西安交通大学第一附属医院普外科;北京大学化学与分子工程学院;
【基金】：国家自然科学基金项目(81101874)资助
【分类号】：O657.33;R735.37
本文编号：2319713
[Abstract]:Fourier transform infrared spectroscopy (FTIR) and principal component analysis (PCA),) were used to analyze the spectral characteristics of metastatic lymph nodes in rectal cancer. The metastatic lymph nodes and non-metastatic lymph nodes of rectal cancer were analyzed by linear discriminant analysis. 80 cases of metastatic lymph nodes of rectal cancer and 80 cases of non-metastatic lymph nodes were analyzed by FTIR spectrum, the peak intensity was calculated and principal component analysis was performed. It is obtained that the principal component of 1 (Principal components 1p1 is 3 260 cm-1 and PC2 is 1 740 cm-1 in the range of 4 000 ~ (-1) cm ~ (-1). The main component is 1 (Principal components ~ (-1) and the PC2 is 1 740 cm ~ (-1) in the range of 4 000 ~ (-1) cm ~ (-1). The wave number is 1 700 ~ (-1) cm ~ (-1), PC1 is 1 640 cm ~ (-1) and PC2 is 1 080 cm ~ (-1). The relative peak / intensity ratio (I/I1 460) and wave numbers of 1 080 and 1 300 cm ~ (-1) of malignant lymph nodes were measured by t test. The difference between benign and malignant results was significant (p0. 05). The results showed that the protein content, protein formation and amino acid increased in metastatic lymph nodes. The decrease of fat content was related to the decrease of anaerobic glycolytic fat content in cancer tissue. The relative peak-to-intensity ratio (I1 080/I1 460G / I1 640/I1 460N / I3 260/I1 460C / I1 740/I1 460NNX 160) was analyzed by PCA cluster analysis. The results showed that benign and malignant lymph nodes could be distinguished from benign lymph nodes in the first and fourth quadrant, and benign lymph nodes could be clustered in the first and fourth quadrants. Malignant lymph nodes were clustered in two and three quadrants. The relative peak / intensity ratio, 1 080 cm ~ (-1) and 1 300 cm ~ (-1) were analyzed by linear discriminant analysis (LDA),). The results showed that the sensitivity and specificity of the PCA/LDA model were 87.5 and 88.5respectively. The results show that Fourier transform infrared spectroscopy (FTIR) can be used as a simple method for the diagnosis of lymph node metastasis of rectal cancer in situ and in vivo.
【作者单位】： 陕西省人民医院普外二科;西安交通大学第一附属医院普外科;北京大学化学与分子工程学院;
【基金】：国家自然科学基金项目(81101874)资助
【分类号】：O657.33;R735.37
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