表阿霉素聚氰基丙烯酸正丁酯磁性纳米粒抑制原位移植性肝癌生长的实验研究

发布时间：2018-11-13 06:30

【摘要】：目的:探讨人肝癌裸鼠原位移植瘤在表阿霉素聚氰基丙烯酸正丁酯磁性纳米粒(EPI-PBCA-MNPS)中的影响及其机制。方法:构建人肝癌裸鼠原位移植瘤模型,并将其分为生理盐水(NS)组、表阿霉素(EPI)组、EPI-BCA-MNPS组和EPI-PBCA-MNPS+磁场(EPI-PBCA-MNPS+MF)组进行实验;用药2周后解剖裸鼠,观察肿瘤体积的变化,计算抑瘤率,并用Western blot、免疫组化法检测肿瘤组织中的增殖细胞核抗原(PCNA),并分析组织中细胞增殖和蛋白的表达情况。结果:治疗后肿瘤体积抑制率各组间差异有统计学意义,EPI-PBCA-MNPS+MF组最高,差异有统计学意义(P0.05)。免疫组化法和Western blot检测显示,EPI-PBCA-MNPS+MF组PCNA蛋白表达明显降低,差异具有统计学意义(P0.05)。结论:表阿霉素聚氰基丙烯酸正丁酯磁性纳米粒具有良好的靶向性,在外加磁场作用下对裸鼠人肝癌模型有显著抑瘤作用,其机制可能是抑制肿瘤细胞的恶性增殖。
[Abstract]:Aim: to investigate the effect of orthotopic transplantation of human hepatocellular carcinoma (HCC) on the magnetic nanoparticles of epirubicin polybutyl cyanoacrylate (EPI-PBCA-MNPS) and its mechanism. Methods: the orthotopic transplanted tumor model of human hepatocellular carcinoma in nude mice was established and divided into normal saline (NS) group, epirubicin (EPI) group, EPI-BCA-MNPS group and EPI-PBCA-MNPS magnetic field (EPI-PBCA-MNPS MF) group. After 2 weeks of administration, nude mice were dissected, tumor volume was observed, tumor inhibition rate was calculated, proliferating cell nuclear antigen (PCNA),) was detected by Western blot, immunohistochemical method, and cell proliferation and protein expression were analyzed. Results: after treatment, there were significant differences in tumor volume inhibition rate among groups, EPI-PBCA-MNPS MF group was the highest, the difference was statistically significant (P0.05). Immunohistochemical method and Western blot detection showed that the expression of PCNA protein in EPI-PBCA-MNPS MF group was significantly lower than that in control group (P0.05). Conclusion: the magnetic nanoparticles of epirubicin polybutylcyanoacrylate have good targeting ability, and have obvious inhibitory effect on human hepatoma model in nude mice under the action of applied magnetic field. The mechanism may be to inhibit the malignant proliferation of tumor cells.
【作者单位】：南通大学附属第三人民医院肝胆外科;
【基金】：南通市科技计划项目(编号:MS12015057)
【分类号】：R735.7

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