肝细胞肝癌患者血清microRNAlet-7a水平的变化及意义

发布时间：2018-11-13 17:07

【摘要】：目的:探讨肝细胞肝癌(HCC)患者血清microRNAlet-7a(let-7a)水平的变化及其对HCC的诊断价值。方法:用qRT-PCR检测60例HCC患者及46例健康体检者血清let-7a的表达,分析血清let-7a水平与HCC的临床病理因素的关系,用受试者工作曲线(ROC)分析其对HCC的诊断效能。结果:HCC患者血清let-7a相对表达量明显低于健康对照组人群(0.538 vs.1.571,P0.05);HCC患者血清let-7a水平达与癌栓形成有关(P0.05),与性别、年龄、HBV感染、肝硬化、肿瘤直径、肿瘤个数、淋巴结转移、TNM分期、淋巴结转移、病理分级、AFP水平均无关(均P0.05);以0.529为let-7a诊断HCC的最佳临界值,其灵敏度为79%,特异度为71%,曲线下面积(AUC)为0.77(95%CI=0.624~0.839);联合检测血清let-7a与AFP,诊断HCC的灵敏度为83%,特异性为97%,AUC为0.92(95%CI=0.866~0.987)。结论:HCC患者血清let-7a水平降低,let-7a可能成为诊断HCC的新的分子标志物,联合AFP检测可提高对HCC的诊断的准确性。
[Abstract]:Objective: to investigate the changes of serum microRNAlet-7a (let-7a) in patients with hepatocellular carcinoma (HCC) and its diagnostic value for HCC. Methods: qRT-PCR was used to detect the expression of serum let-7a in 60 patients with HCC and 46 healthy controls. The relationship between serum let-7a level and clinicopathological factors of HCC was analyzed. The diagnostic efficacy of (ROC) on HCC was analyzed by using the operating curve (ROC). Results: the relative expression of serum let-7a in patients with HCC was significantly lower than that in healthy controls (0.538 vs.1.571,P0.05). Serum let-7a levels in HCC patients were correlated with tumor thrombus formation (P0.05), sex, age, HBV infection, liver cirrhosis, tumor diameter, tumor number, lymph node metastasis, TNM stage, lymph node metastasis, pathological grade. The level of AFP was not significant (P0.05). Using 0.529 as the best critical value for the diagnosis of HCC, the sensitivity was 79 and the specificity was 71. The area under the curve (AUC) was 0.77 (95%CI=0.624~0.839). The sensitivity and specificity of combined detection of serum let-7a and AFP, for HCC were 83 and 0.92 (95%CI=0.866~0.987). Conclusion: the level of serum let-7a in HCC patients is lower and let-7a may be a new molecular marker for the diagnosis of HCC. Combined with AFP detection can improve the diagnostic accuracy of HCC.
【作者单位】：湖南师范大学第一附属医院/湖南省人民医院胆道外科;
【基金】：湖南省长沙市科学技术局科技计划资助项目(K1203042-31)
【分类号】：R735.7

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