β-catenin、NCAM蛋白在甲状腺癌组织的表达及其意义
发布时间：2018-11-18 16:42
【摘要】：目的:研究β-连环素蛋白(β-catenin)、神经细胞黏附分子(neural cell adhesion molecule,NCAM)蛋白在甲状腺癌(thyroid carcinoma,TC)组织的表达,对比分析甲状腺癌组织与甲状腺良性病变组织中β-catenin、NCAM蛋白的表达差异,探讨β-catenin、NCAM蛋白在甲状腺癌发生、发展中的意义,同时初步探讨β-catenin、NCAM蛋白在甲状腺癌侵袭、转移中的作用。方法:收集临沂市人民医院2008年至2015年保存的甲状腺癌组织存档蜡块,所有病例诊断明确,临床资料完整,术前均未作特别治疗。取70例甲状腺癌组织和36例甲状腺良性病变组织。用免疫组化链霉素抗生物素-过氧化物酶法(S-P法)联合检测β-catenin、NCAM蛋白在标本中的表达,探讨NCAM、β-catenin表达与患者临床病理特征的关系。应用统计软件SPSS19.0分析,采用X 2检验,Spearman等级相关分析,P0.05为差异有统计学意义。结果:1.在甲状腺良性病变组织中,β-catenin蛋白主要表达于细胞膜,而在甲状腺癌组织中,β-catenin蛋白在组织胞质或胞核异位表达,在细胞膜的阳性表达率明显低于甲状腺良性病变组织。β-catenin在甲状腺癌组织中的异常表达率高于甲状腺良性病变组织(P均0.05)。甲状腺癌各组织学类型(包括甲状腺乳头状癌、甲状腺滤泡癌、甲状腺髓样癌和未分化甲状腺癌)中β-catenin异常表达率差异,经X 2检验无统计学意义(P0.05);甲状腺癌组织中β-catenin蛋白异常表达率与患者的性别、年龄、肿瘤直径、病理分期以及周围淋巴结有无转移均无关(P均0.05)。2.NCAM阳性表达主要位于细胞膜。NCAM在甲状腺癌组织中的阳性表达率低于甲状腺良性病变组织(P0.05)。甲状腺癌各组织学类型中NCAM表达的阳性率差异,经X 2检验无统计学意义(P0.05);NCAM阳性率在周围淋巴结有无转移之间,经X 2检验差异有统计学意义(P0.05);甲状腺癌组织中NCAM阳性率与患者的性别、年龄、肿瘤直径以及病理分期均无关(P均0.05)。3.甲状腺癌组织中NCAM的阳性表达与β-catenin异常表达经Spearman等级相关分析,呈负相关(r=-0.025,P0.05)。结论:1.β-catenin在甲状腺癌中存在普遍的异位表达,细胞膜的表达率降低,而细胞质或细胞核表达率增高,提示β-catenin异常表达在甲状腺癌发生、发展中可能发挥的重要作用;2.甲状腺癌中NCAM阳性率相对于良性甲状腺病变显著降低;NCAM的表达率与淋巴结转移密切相关,提示NCAM可能是甲状腺癌转移信号通路中发挥重要作用的分子,与甲状腺癌侵袭转移密切相关;3.β-catenin、NCAM的检测对于未分化甲状腺癌与甲状腺乳头状癌、甲状腺滤泡癌、甲状腺髓样癌的鉴别诊断无重要价值;在鉴别甲状腺癌与良性甲状腺病变中,β-catenin、NCAM联合检测可以提高诊断的准确性;4.β-catenin、NCAM两种蛋白表达呈负相关,这种反向平衡现象,提示NCAM、β-catenin在甲状腺癌的发生、发展、浸润及转移中可能密切相关。
[Abstract]:Objective: to study the expression of 尾 -catenin (尾 -catenin) (neural cell adhesion molecule,NCAM in thyroid carcinoma (thyroid carcinoma,TC). To compare and analyze the difference of 尾-catenin,NCAM protein expression between thyroid carcinoma and benign thyroid lesions, to explore the significance of 尾-catenin,NCAM protein in the occurrence and development of thyroid carcinoma, and to explore the 尾-catenin, protein expression at the same time. Role of NCAM protein in invasion and Metastasis of thyroid carcinoma. Methods: the paraffin lumps of thyroid cancer tissue were collected from 2008 to 2015 in Linyi people's Hospital. All the cases were diagnosed clearly and the clinical data were complete. No special treatment was given before operation. 70 cases of thyroid carcinoma and 36 cases of benign thyroid lesions were collected. The expression of 尾-catenin,NCAM protein was detected by immunohistochemical streptomycin biotin-peroxidase (S-P) method, and the relationship between the expression of NCAM, 尾-catenin and the clinicopathological features of the patients was investigated. Statistical software SPSS19.0 analysis, using X 2 test, Spearman grade correlation analysis, P0.05 for the difference was statistically significant. The result is 1: 1. In benign thyroid lesions, 尾-catenin protein is mainly expressed in cell membrane, while in thyroid carcinoma, 尾-catenin protein is expressed in cytoplasm or nuclear ectopic. The positive expression rate of 尾-catenin in thyroid cancer tissues was significantly lower than that in benign thyroid lesions (P < 0. 05), and the abnormal expression rate of 尾-catenin in thyroid cancer tissues was higher than that in thyroid benign lesions (all P 0. 05). The abnormal expression rate of 尾-catenin in thyroid carcinoma tissues (including papillary thyroid carcinoma, thyroid follicular carcinoma, medullary thyroid carcinoma and undifferentiated thyroid carcinoma) showed no significant difference (P0.05). The abnormal expression rate of 尾-catenin protein in thyroid carcinoma was correlated with sex, age, tumor diameter, The positive expression of 2.NCAM was mainly located in the cell membrane. The positive expression rate of NCAM in thyroid carcinoma was lower than that in benign thyroid lesions (P0.05). There was no significant difference in the positive rate of NCAM expression among different histological types of thyroid carcinoma by X2 test (P0.05). (P0.05) the positive rate of); NCAM was significantly different between the peripheral lymph node metastasis and X2 test (P0.05). The positive rate of NCAM in thyroid carcinoma was not correlated with sex, age, tumor diameter and pathological stage (P < 0. 05). The positive expression of NCAM and the abnormal expression of 尾-catenin were negatively correlated with the expression of 尾-catenin in thyroid carcinomas by Spearman grade correlation analysis (P 0.05). Conclusion: 1. There is a general ectopic expression of 尾-catenin in thyroid carcinoma, the expression rate of cell membrane is decreased, while the expression rate of cytoplasm or nucleus is increased, suggesting that the abnormal expression of 尾-catenin is found in thyroid carcinoma. The important role that could be played in development; 2. The positive rate of NCAM in thyroid carcinoma was significantly lower than that in benign thyroid lesions. The expression rate of NCAM is closely related to lymph node metastasis, suggesting that NCAM may play an important role in the signal pathway of thyroid cancer metastasis, and is closely related to the invasion and metastasis of thyroid carcinoma. 3. The detection of 尾-catenin,NCAM has no significant value in the differential diagnosis of undifferentiated thyroid carcinoma from thyroid papillary carcinoma, thyroid follicular carcinoma and medullary thyroid carcinoma. In the differential diagnosis of thyroid carcinoma and benign thyroid disease, 尾-catenin,NCAM combined detection can improve the accuracy of diagnosis. 4. The expression of 尾-catenin,NCAM is negatively correlated with the reverse balance, suggesting that NCAM, 尾-catenin may be closely related to the occurrence, development, invasion and metastasis of thyroid carcinoma.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R736.1
本文编号：2340556
[Abstract]:Objective: to study the expression of 尾 -catenin (尾 -catenin) (neural cell adhesion molecule,NCAM in thyroid carcinoma (thyroid carcinoma,TC). To compare and analyze the difference of 尾-catenin,NCAM protein expression between thyroid carcinoma and benign thyroid lesions, to explore the significance of 尾-catenin,NCAM protein in the occurrence and development of thyroid carcinoma, and to explore the 尾-catenin, protein expression at the same time. Role of NCAM protein in invasion and Metastasis of thyroid carcinoma. Methods: the paraffin lumps of thyroid cancer tissue were collected from 2008 to 2015 in Linyi people's Hospital. All the cases were diagnosed clearly and the clinical data were complete. No special treatment was given before operation. 70 cases of thyroid carcinoma and 36 cases of benign thyroid lesions were collected. The expression of 尾-catenin,NCAM protein was detected by immunohistochemical streptomycin biotin-peroxidase (S-P) method, and the relationship between the expression of NCAM, 尾-catenin and the clinicopathological features of the patients was investigated. Statistical software SPSS19.0 analysis, using X 2 test, Spearman grade correlation analysis, P0.05 for the difference was statistically significant. The result is 1: 1. In benign thyroid lesions, 尾-catenin protein is mainly expressed in cell membrane, while in thyroid carcinoma, 尾-catenin protein is expressed in cytoplasm or nuclear ectopic. The positive expression rate of 尾-catenin in thyroid cancer tissues was significantly lower than that in benign thyroid lesions (P < 0. 05), and the abnormal expression rate of 尾-catenin in thyroid cancer tissues was higher than that in thyroid benign lesions (all P 0. 05). The abnormal expression rate of 尾-catenin in thyroid carcinoma tissues (including papillary thyroid carcinoma, thyroid follicular carcinoma, medullary thyroid carcinoma and undifferentiated thyroid carcinoma) showed no significant difference (P0.05). The abnormal expression rate of 尾-catenin protein in thyroid carcinoma was correlated with sex, age, tumor diameter, The positive expression of 2.NCAM was mainly located in the cell membrane. The positive expression rate of NCAM in thyroid carcinoma was lower than that in benign thyroid lesions (P0.05). There was no significant difference in the positive rate of NCAM expression among different histological types of thyroid carcinoma by X2 test (P0.05). (P0.05) the positive rate of); NCAM was significantly different between the peripheral lymph node metastasis and X2 test (P0.05). The positive rate of NCAM in thyroid carcinoma was not correlated with sex, age, tumor diameter and pathological stage (P < 0. 05). The positive expression of NCAM and the abnormal expression of 尾-catenin were negatively correlated with the expression of 尾-catenin in thyroid carcinomas by Spearman grade correlation analysis (P 0.05). Conclusion: 1. There is a general ectopic expression of 尾-catenin in thyroid carcinoma, the expression rate of cell membrane is decreased, while the expression rate of cytoplasm or nucleus is increased, suggesting that the abnormal expression of 尾-catenin is found in thyroid carcinoma. The important role that could be played in development; 2. The positive rate of NCAM in thyroid carcinoma was significantly lower than that in benign thyroid lesions. The expression rate of NCAM is closely related to lymph node metastasis, suggesting that NCAM may play an important role in the signal pathway of thyroid cancer metastasis, and is closely related to the invasion and metastasis of thyroid carcinoma. 3. The detection of 尾-catenin,NCAM has no significant value in the differential diagnosis of undifferentiated thyroid carcinoma from thyroid papillary carcinoma, thyroid follicular carcinoma and medullary thyroid carcinoma. In the differential diagnosis of thyroid carcinoma and benign thyroid disease, 尾-catenin,NCAM combined detection can improve the accuracy of diagnosis. 4. The expression of 尾-catenin,NCAM is negatively correlated with the reverse balance, suggesting that NCAM, 尾-catenin may be closely related to the occurrence, development, invasion and metastasis of thyroid carcinoma.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R736.1
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