SD大鼠肝癌模型建立及ICG-001在大鼠诱发性肝癌中对Survivin的影响

发布时间：2018-11-25 19:30

【摘要】：目的:构造SD大鼠肝癌模型,用β-catenin抑制剂ICG-001活体干预已成瘤的SD大鼠,探讨β-catenin抑制剂ICG-001对SD大鼠肝癌组织中凋亡抑制蛋白Survivin的影响。方法:予二乙基亚硝胺(DEN)喂养SD大鼠,随后将已成瘤的大鼠分成肿瘤组,DMSO组和给药组。给药组大鼠予ICG-001腹腔注射抑制β-catenin的表达后,Western blot检测ICG-001对大鼠肝癌组织中Survivin蛋白表达的影响,半定量RT-PCR检测ICG-001对大鼠肝癌组织中Survivin的mRNA的影响。分别用Image J软件和凝胶成像分析系统检测Survivin的蛋白和mRNA表达水平,测量条带的灰度值,将其转化为数据,并使用SPSS.18进行统计学分析。结果:予二乙基亚硝胺喂养SD大鼠,构造肝癌模型成功,成瘤率约为83.3%。用ICG-001抑制β-catenin的表达后,Western blot结果显示:给药组Survivin的表达明显低于对照组,且差异有统计学意义,p=0.0010.05;而对照组和DMSO组组间差异没有统计学意义,p=0.4280.05;即ICG-001给药后Survivin的蛋白表达量降低。半定量RT-PCR结果显示:给药组Survivin的mRNA表达含量较对照组明显减低,且差异有统计学意义:p=0.0360.05;而对照组和DMSO组组间差异没有统计学意义,p=0.4560.05;即ICG-001给药后Survivin的mRNA表达量降低。结论:予二乙基亚硝胺喂养SD大鼠,构造肝癌模型成功,成瘤率约为83.3%。β-catenin抑制剂ICG-001明显抑制SD大鼠肝癌中Survivin的蛋白表达及mRNA表达。
[Abstract]:Aim: to construct a SD rat model of hepatocellular carcinoma, and to investigate the effect of 尾 catenin inhibitor ICG-001 on apoptosis inhibitor Survivin in SD rat liver cancer tissue by using 尾-catenin inhibitor ICG-001 in vivo to interfere with SD rats. Methods: SD rats were fed with diethylnitrosamine (DEN), then the rats were divided into three groups: tumor group, DMSO group and administration group. After intraperitoneal injection of ICG-001 to inhibit the expression of 尾-catenin, Western blot was used to detect the effect of ICG-001 on the expression of Survivin protein in rat liver cancer, and semi-quantitative RT-PCR was used to detect the effect of ICG-001 on Survivin mRNA in rat liver cancer. The protein and mRNA expression levels of Survivin were detected by Image J software and gel imaging analysis system. The gray values of the bands were measured and converted into data. The results were analyzed statistically by SPSS.18. Results: SD rats were fed with diethylnitrosamine and the liver cancer model was successfully constructed. The tumorigenesis rate was about 83.3%. The results of, Western blot showed that the expression of 尾-catenin in the administration group was significantly lower than that in the control group, and the difference was statistically significant (p0. 0010.05), but there was no significant difference between the control group and the DMSO group (p0. 428. 05). That is, the protein expression of Survivin decreased after ICG-001 administration. The results of semi-quantitative RT-PCR showed that the mRNA expression of Survivin in the administration group was significantly lower than that in the control group, and the difference was statistically significant: p0. 036. 05. 05. 05. 05. 05. 05. 05. 05. 05. 05. 05. 05. 05. 05. 05. 05. 05. 05. 05. 05. 05. 05. 05. 0. 05. That is, the mRNA expression of Survivin decreased after ICG-001 administration. Conclusion: SD rats were fed with diethylnitrosamine and the tumor formation rate was about 83.3%. 尾-catenin inhibitor ICG-001 significantly inhibited the expression of Survivin protein and mRNA in SD rats.
【学位授予单位】：桂林医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R735.7;R-332

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