体外扩增人脐带血来源的NK细胞对食管癌细胞的促凋亡作用
发布时间：2018-11-28 13:49
【摘要】：目的:建立体外扩增人脐带血来源的NK细胞的方法,并研究其对多株人食管癌细胞系和食管癌患者原代肿瘤细胞的杀伤作用。方法:分离人脐带血单个核细胞,加入放射线灭活的人白血病K562细胞及IL-2、IL-15、IL-18细胞因子体外扩增培养2周,诱导NK细胞。采用流式细胞术检测NK细胞的扩增效果及其产生的IFN-γ等细胞因子杀伤靶细胞K562的能力,并检测体外扩增的人脐带血来源NK细胞对多种食管癌细胞系和食管癌患者原代肿瘤细胞凋亡的影响。结果:利用放射线灭活的K562细胞联合多种细胞因子,可以有效的扩增人脐带血来源的NK细胞。体外培养2周后NK细胞比率可达80%左右,并对靶细胞K562有明显促凋亡作用。此外,体外扩增的人脐带血来源NK细胞能够促进6株人食管癌细胞系和食管癌患者原代肿瘤细胞的凋亡。结论:本研究建立了体外扩增人脐带血NK细胞的方法,并证实这些NK细胞对食管癌细胞的促凋亡作用,可能为食管癌的免疫治疗提供新的手段。
[Abstract]:Aim: to establish a method to amplify NK cells derived from human umbilical cord blood in vitro and to study its killing effect on human esophageal cancer cell lines and primary tumor cells from esophageal cancer patients. Methods: human umbilical cord blood mononuclear cells were isolated and cultured in vitro with radiation-inactivated human leukemia K562 cells and IL-2,IL-15,IL-18 cytokines for 2 weeks to induce NK cells. Flow cytometry was used to detect the amplification effect of NK cells and the ability of IFN- 纬 and other cytokines to kill K562 target cells. The effects of NK cells derived from human umbilical cord blood on apoptosis of various esophageal cancer cell lines and primary tumor cells from patients with esophageal carcinoma were examined. Results: NK cells derived from human umbilical cord blood could be effectively amplified by radiation inactivated K562 cells combined with many cytokines. After 2 weeks of culture in vitro, the ratio of NK cells was about 80%, and the target cell K562 could induce apoptosis. In addition, NK cells derived from human umbilical cord blood in vitro could promote apoptosis of 6 human esophageal cancer cell lines and primary tumor cells of esophageal cancer patients. Conclusion: this study established a method to amplify human umbilical cord blood NK cells in vitro, and confirmed that these NK cells can promote apoptosis of esophageal cancer cells, which may provide a new method for immunotherapy of esophageal cancer.
【作者单位】： 郑州大学第一附属医院生物细胞治疗中心;郑州大学第一临床学院;郑州大学生命科学学院;
【基金】：河南省基础与前沿技术研究计划基金资助项目(No.142300410387.0)~~
【分类号】：R735.1
本文编号：2363015
[Abstract]:Aim: to establish a method to amplify NK cells derived from human umbilical cord blood in vitro and to study its killing effect on human esophageal cancer cell lines and primary tumor cells from esophageal cancer patients. Methods: human umbilical cord blood mononuclear cells were isolated and cultured in vitro with radiation-inactivated human leukemia K562 cells and IL-2,IL-15,IL-18 cytokines for 2 weeks to induce NK cells. Flow cytometry was used to detect the amplification effect of NK cells and the ability of IFN- 纬 and other cytokines to kill K562 target cells. The effects of NK cells derived from human umbilical cord blood on apoptosis of various esophageal cancer cell lines and primary tumor cells from patients with esophageal carcinoma were examined. Results: NK cells derived from human umbilical cord blood could be effectively amplified by radiation inactivated K562 cells combined with many cytokines. After 2 weeks of culture in vitro, the ratio of NK cells was about 80%, and the target cell K562 could induce apoptosis. In addition, NK cells derived from human umbilical cord blood in vitro could promote apoptosis of 6 human esophageal cancer cell lines and primary tumor cells of esophageal cancer patients. Conclusion: this study established a method to amplify human umbilical cord blood NK cells in vitro, and confirmed that these NK cells can promote apoptosis of esophageal cancer cells, which may provide a new method for immunotherapy of esophageal cancer.
【作者单位】： 郑州大学第一附属医院生物细胞治疗中心;郑州大学第一临床学院;郑州大学生命科学学院;
【基金】：河南省基础与前沿技术研究计划基金资助项目(No.142300410387.0)~~
【分类号】：R735.1
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