血浆长链非编码RNA FENDRR、H19在宣威肺癌中的潜在诊断价值研究

发布时间：2018-11-29 07:14

【摘要】：[目的]本研究通过检测宣威肺癌、非宣威肺癌、宣威肺良性病、健康对照四组人群血浆中FENDRR、H19基因的表达水平,研究FENDRR、H19在宣威肺癌患者血浆中的变化趋势及临床意义,评价FENDRR、H19在宣威肺癌患者中的潜在诊断价值。[方法]收集2015年6月至2016年8月昆明医科大学第一附属医院患者术前以及性别年龄匹配的体检健康者血浆,共163例样本。样本分宣威肺癌、非宣威肺癌、宣威良性肺疾病、健康对照4组,其中宣威肺癌43例,其它3组每组各40例。采用实时荧光定量PCR方法检测研究对象血浆中FENDRR、H19基因的表达水平。并对宣威肺癌患者临床病例资料进行统计分析,分析FENDRR、H19表达水平与患者性别、年龄、吸烟情况、TNM分期、淋巴结转移情况、肿块大小和组织学类型的相关性,评价血浆FENDRR和H19作为宣威肺癌筛查标志物的诊断价值。[结果]与对照组相比,FENDRR、H19在宣威肺癌组、非宣威肺癌组中表达水平整体呈下调趋势(P0.05)。FENDRR、H19的表达水平与患者性别、年龄、吸烟史、TNM分期、组织学类型、肿块大小均无相关性(P0.05);H19的表达水平与患者淋巴结转移情况有相关性(P0.05)。NSE的AUC值为0.648,敏感性和特异性分别为52.6%和78.9%;CEA的AUC值为0.759,敏感性和特异性分别为68.4%和 73.7%;联合检测 NSE 和 CEA 时,AUC 值为 0.778。FENDRR的AUC值为 0.812,敏感性和特异性分别为90.7%和75%;H19的AUC值为0.833,敏感性和特异性分别为74.4%和85%;而当联合检测FENDRR和H19时,AUC值为0.894。[结论]FENDRR、H19在宣威肺癌的发生、发展中发挥着重要作用,它们可以作为宣威肺癌潜在的诊断标志物,而且诊断价值优于传统标志物。
[Abstract]:[objective] to investigate the expression of FENDRR,H19 gene in the plasma of Xuanwei lung cancer, non-Xuanwei lung cancer, Xuanwei lung benign venereal disease and healthy control group, and to study the change trend and clinical significance of FENDRR,H19 in the plasma of Xuanwei lung cancer patients. To evaluate the potential diagnostic value of FENDRR,H19 in Xuanwei lung cancer patients. Methods from June 2015 to August 2016, a total of 163 plasma samples were collected from patients in the first affiliated Hospital of Kunming Medical University. The samples were divided into 4 groups: Xuanwei lung cancer, non-Xuanwei lung cancer, Xuanwei benign lung disease, healthy control group, 43 cases of Xuanwei lung cancer, 40 cases each of the other three groups. Real-time fluorescence quantitative PCR was used to detect the expression of FENDRR,H19 gene in plasma. The correlation between FENDRR,H19 expression and sex, age, smoking status, TNM stage, lymph node metastasis, tumor size and histological type was analyzed. To evaluate the diagnostic value of plasma FENDRR and H 19 as screening markers of Xuan Wei lung cancer. [results] compared with the control group, the expression of FENDRR,H19 in Xuanwei lung cancer group and non-Xuanwei lung cancer group showed a downward trend (P0.05). The expression level of FENDRR,H19 was correlated with sex, age, smoking history, TNM stage and histological type. There was no correlation between tumor size and tumor size (P0.05). The expression of H19 was correlated with lymph node metastasis (P0.05 AUC value of). NSE was 0.648, sensitivity and specificity were 52.6% and 78.9%, respectively); The AUC value of CEA was 0.759, the sensitivity and specificity were 68.4% and 73.7%, the AUC value of 0.778.FENDRR was 0.812, the sensitivity and specificity were 90.7% and 75%, respectively. The AUC value of H19 was 0.833, the sensitivity and specificity of H19 were 74.4% and 85.When combined detection of FENDRR and H19, the AUC value was 0.894. [conclusion] FENDRR,H19 plays an important role in the occurrence and development of Xuanwei lung cancer. They can be used as potential diagnostic markers of Xuanwei lung cancer, and the diagnostic value is superior to that of traditional markers.
【学位授予单位】：昆明医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R734.2

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