长链非编码RNA-ROR在乳腺癌中的表达及临床意义研究

发布时间：2018-12-13 16:26

【摘要】：目的探讨长链非编码RNA-ROR(Large intergenic non-coding RNA-ROR,Linc RNA-ROR)在乳腺癌患者血清与正常人血清中的表达差异及临床意义。方法采用荧光定量-逆转录PCR(q RT-PCR)的方法检测96例乳腺癌患者和90例健康体检者血清中lincRNA-ROR的表达水平,同时分析lincRNA-ROR的表达水平与乳腺癌临床病理资料的相关性。结果乳腺癌患者血清中lincRNA-ROR的表达水平高于健康体检者血清中的表达水平[(1.88±0.73)vs.(1.31±0.62)],差异有统计学意义(P0.05);血清lincRNA-ROR在淋巴结转移患者中的表达水平高于无淋巴结转移者[(2.18±1.09)vs.(1.66±1.18)],差异有统计学意义(P0.05);雌激素受体阳性(ER+)和孕激素受体阳性(PR+)癌症患者血清中lincRNA-ROR的表达水平均高于雌激素受体阴性(ER-)和孕激素受体阴性(PR-)的患者,其相对表达量分别为[(2.13±1.13)vs.(1.62±1.08)]和[(2.22±0.95)vs.(1.78±1.04)],差异有统计学意义(P均0.05);而血清中lincRNA-ROR的表达水平与患者年龄、病理类型、肿瘤TNM分期、细胞增殖指数(Ki-67)和人表皮生长因子受体-2(HER-2)状态不具有相关性。结论 Linc RNA-ROR在乳腺癌患者血清中表达上调,其表达水平与雌激素受体和孕激素受体状态以及淋巴结转移相关,提示lincRNA-ROR可能成为潜在的乳腺癌诊断的分子标记物。
[Abstract]:Objective to investigate the difference and clinical significance of long chain non coding RNA-ROR (Large intergenic non-coding RNA-ROR,Linc RNA-ROR) expression in serum of breast cancer patients and normal controls. Methods the expression of lincRNA-ROR in serum of 96 patients with breast cancer and 90 healthy controls was detected by fluorescence quantitative reverse transcriptase (PCR (q RT-PCR). The correlation between the expression of lincRNA-ROR and the clinicopathological data of breast cancer was also analyzed. Results the expression level of lincRNA-ROR in serum of breast cancer patients was higher than that of healthy controls (1.88 卤0.73) vs. (1.31 卤0.62), and the difference was statistically significant (P0.05). The expression of serum lincRNA-ROR in patients with lymph node metastasis was significantly higher than that in patients without lymph node metastasis (2.18 卤1.09) vs. (1.66 卤1.18) (P0.05). The expression of lincRNA-ROR in serum of cancer patients with estrogen receptor positive (ER) and progesterone receptor positive (PR) was higher than that of estrogen receptor negative (ER-) and progesterone receptor negative (PR-) patients. Their relative expression levels were (2.13 卤1.13) vs. (1.62 卤1.08) and (2.22 卤0.95) vs. (1.78 卤1.04), respectively. However, the expression of lincRNA-ROR in serum was not correlated with age, pathological type, tumor TNM stage, cell proliferation index (Ki-67) and human epidermal growth factor receptor-2 (HER-2) status. Conclusion the expression of Linc RNA-ROR is up-regulated in serum of breast cancer patients, and its expression level is related to the status of estrogen receptor and progesterone receptor and lymph node metastasis, suggesting that lincRNA-ROR may be a potential molecular marker for breast cancer diagnosis.
【作者单位】：四川省肿瘤医院;四川大学华西公共卫生学院(华西第四医院);
【基金】：国家自然科学基金面上项目(No.81372945)
【分类号】：R737.9

【相似文献】