包裹全氟戊烷空心磁性聚合物微球对微波消融效果影响的实验研究
发布时间：2018-12-15 09:08
【摘要】：目的1、制备一种新型空心磁性聚合物微球(Hollow magnetic polymer microspere, HMPMS),并将相变材料全氟戊烷(Perfluoropentane, PFP)包裹于其内,检测其基本特性,并通过体外实验验证其相变及对微波消融(Microwave ablation, MWA)效果的影响。2、研究自制包裹PFP的空心磁性聚合物微球(PFP-HMPMS)被HepG2肝癌细胞吞噬的能力,并对其细胞毒性进行评价。研究PFP-HMPMS增强HepG2肝癌细胞MWA效果的可行性。3、通过在体实验研究自制PFP-HMPMS增效裸鼠皮下HepG2移植瘤模型MWA效果的可行性。材料和方法1、采用模板沉积法制备HMPMS,对其外观、粒径进行分析。研究不同温度促进其相变的情况及PFP-HMPMS对MWA温升速率的影响。2、 用普鲁士蓝染色观察HepG2细胞对PFP-HMPMS的吞噬能力；用四唑盐(Methyl thiazolyl tetrazolium, MTT)比色法检测PFP-HMPMS的细胞毒性；用流式细胞仪检测HepG2细胞吞噬PFP-HMPMS后MWA在不同温控条件下的细胞存活状态的比例,并与对照组相比较。3、建立裸鼠皮下HepG2移植瘤模型,对其行MWA治疗,比较局部注射PFP-HMPMS的实验组与对照组温升速率的差异,并通过组织病理学检查考察PFP-HMPMS增强MWA治疗肿瘤的效果。结果1、本研究制备出的HMPMS球形度较好、粒径均一,且包裹PFP后,可在温度超过40℃时发生相变,并能在微波(Microwave, MW)场中增强MW能量的吸收而快速升温。2、自制的HMPMS可被HepG2细胞所吞噬,标记阳性率为71.7士3.5%；当PFP-HMPMS浓度为100μg/ml时对HepG2细胞的生存具有轻微的生长抑制作用(P0.05);MWA2h后流式结果显示HepG2细胞吞噬PFP-HMPMS后,当加热温度为45℃、50℃及60℃时,正常存活细胞及早期凋亡细胞的百分比明显降低；而50℃及60℃时,HepG2细胞早期凋亡、晚期凋亡或死亡的比例明显提高(P0.05)。3、实验组荷瘤裸鼠局部注射PFP-HMPMS后,温度升高到50℃所需要的时间与对照组相比差别具有统计学意义(P0.05)；组织病理学结果显示PFP-HMPMS沉积区域肿瘤细胞结构变化显著。结论1、自制的PFP-HMPMS能够通过温度的升高发生相变,并且在MW场中可增强MW能量的吸收而快速升温。2、自制的HMPMS可以被HepG2细胞所吞噬；PFP-HMPMS对HepG2细胞增殖影响较小；在相同温控条件下,PFP-HMPMS具有增强肿瘤细胞凋亡和死亡的比例的作用。3、自制的PFP-HMPMS能够提高MWA温升效率,增强MWA对在体HepG2皮下移植瘤的治疗效果。
[Abstract]:Objective 1. To prepare a new hollow magnetic polymer microsphere (Hollow magnetic polymer microspere, HMPMS),) and encapsulate the phase change material perfluoropentane (Perfluoropentane, PFP) in it. The phase change and the effect of microwave ablation on (Microwave ablation, MWA) were tested in vitro. 2. The ability of self-made hollow magnetic polymer microspheres (PFP-HMPMS) coated with PFP to be swallowed by HepG2 hepatoma cells was studied. Its cytotoxicity was evaluated. To study the feasibility of PFP-HMPMS to enhance the MWA effect of HepG2 hepatoma cells. 3. To study the effect of self-made PFP-HMPMS on the effect of subcutaneous HepG2 transplantation in nude mice with MWA in vivo. Materials and methods 1. HMPMS, was prepared by template deposition method to analyze its appearance and particle size. The effect of PFP-HMPMS on the temperature rise rate of MWA was studied. 2 the phagocytosis of HepG2 cells to PFP-HMPMS was observed by Prussian blue staining. Cytotoxicity of PFP-HMPMS was detected by tetrazolium (Methyl thiazolyl tetrazolium, MTT) colorimetry. Flow cytometry was used to detect the survival rate of MWA after PFP-HMPMS phagocytosis by HepG2 cells under different temperature control conditions. Compared with the control group, a subcutaneous HepG2 transplanted tumor model was established in nude mice and treated with MWA. The difference of temperature rise rate between the experimental group and the control group was compared, and the effect of PFP-HMPMS enhanced MWA on tumor was investigated by histopathological examination. Results 1. The HMPMS prepared in this study has good sphericity and uniform particle size, and after encapsulating PFP, the phase transition can take place when the temperature exceeds 40 鈩，
本文编号：2380387
[Abstract]:Objective 1. To prepare a new hollow magnetic polymer microsphere (Hollow magnetic polymer microspere, HMPMS),) and encapsulate the phase change material perfluoropentane (Perfluoropentane, PFP) in it. The phase change and the effect of microwave ablation on (Microwave ablation, MWA) were tested in vitro. 2. The ability of self-made hollow magnetic polymer microspheres (PFP-HMPMS) coated with PFP to be swallowed by HepG2 hepatoma cells was studied. Its cytotoxicity was evaluated. To study the feasibility of PFP-HMPMS to enhance the MWA effect of HepG2 hepatoma cells. 3. To study the effect of self-made PFP-HMPMS on the effect of subcutaneous HepG2 transplantation in nude mice with MWA in vivo. Materials and methods 1. HMPMS, was prepared by template deposition method to analyze its appearance and particle size. The effect of PFP-HMPMS on the temperature rise rate of MWA was studied. 2 the phagocytosis of HepG2 cells to PFP-HMPMS was observed by Prussian blue staining. Cytotoxicity of PFP-HMPMS was detected by tetrazolium (Methyl thiazolyl tetrazolium, MTT) colorimetry. Flow cytometry was used to detect the survival rate of MWA after PFP-HMPMS phagocytosis by HepG2 cells under different temperature control conditions. Compared with the control group, a subcutaneous HepG2 transplanted tumor model was established in nude mice and treated with MWA. The difference of temperature rise rate between the experimental group and the control group was compared, and the effect of PFP-HMPMS enhanced MWA on tumor was investigated by histopathological examination. Results 1. The HMPMS prepared in this study has good sphericity and uniform particle size, and after encapsulating PFP, the phase transition can take place when the temperature exceeds 40 鈩，
本文编号：2380387
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