鼠尾草酸联合他莫昔芬通过激活Caspase-3信号通路诱导乳腺癌细胞凋亡

发布时间：2018-12-18 13:01

【摘要】：目的探讨鼠尾草酸联合他莫昔芬对乳腺癌细胞的影响及作用机制。方法将乳腺癌细胞株MCF-7、T47D分为4组,对照组加入PBS,CA组加入鼠尾草酸,TAM组加入他莫昔芬,CA+TAM组加入鼠尾草酸和他莫昔芬。MTT增殖实验检测细胞增殖能力,集落形成实验检测细胞集落形成能力,Transwell小室实验检测细胞侵袭能力,细胞划痕实验检测细胞迁移能力,免疫印迹法检测各组细胞Caspase-8、Caspase-3、PARP蛋白表达。结果在0~20μmol/L药物浓度时,CA、TAM、CA+TAM呈剂量依赖性抑制乳腺癌细胞增殖,但药物浓度为20μmol/L与50μmol/L对细胞增殖影响差异无统计学意义(均P0.05)。CA+TAM组增殖抑制率明显高于CA组和TAM组(均P0.05)。MCF-7和T47D细胞中,CA组、TAM组、CA+TAM组集落形成率、侵袭细胞数、细胞迁移率显著低于对照组(均P0.05);CA+TAM组集落形成率、侵袭细胞数、细胞迁移率显著低于CA组和TAM组(均P0.05),CA组和TAM组集落形成率、侵袭细胞数、细胞迁移率比较差异均无统计学意义(均P0.05)。在2种乳腺癌细胞株中,对照组、CA组、TAM组、CA+TAM组cleaved Caspase-8蛋白表达差异无统计学意义(均P0.05),CA组、TAM组、CA+TAM组cleaved Caspase-3、cleaved PARP蛋白表达显著高于对照组(均P0.05),CA+TAM组cleaved Caspase-3、cleaved PARP蛋白表达显著高于CA组和TAM组(均P0.05),CA组和TAM组cleaved Caspase-3、cleaved PARP蛋白表达差异无统计学意义(均P0.05)。结论鼠尾草酸联合他莫昔芬能够抑制乳腺癌细胞的增殖、迁移和侵袭,其作用机制可能与激活Caspase-3/PARP信号通路有关。
[Abstract]:Objective to investigate the effect and mechanism of salicylic acid combined with tamoxifen on breast cancer cells. Methods Breast cancer cell line MCF-7,T47D was divided into four groups. The control group was added with PBS,CA group and the TAM group was added with tamoxifen, CA TAM group. The proliferation ability of the cells was measured by MTT proliferation assay. Colony forming assay was used to detect cell colony formation ability, Transwell chamber assay to detect cell invasion ability, cell scratch assay to detect cell migration ability, and Western blot method to detect the expression of Caspase-8,Caspase-3,PARP protein. Results at 0 渭 mol/L concentration, CA,TAM,CA TAM inhibited the proliferation of breast cancer cells in a dose-dependent manner. However, there was no significant difference between 20 渭 mol/L and 50 渭 mol/L in cell proliferation (P 0.05). The inhibitory rate of proliferation in). CA TAM group was significantly higher than that in CA group and TAM group (P0.05). The colony formation rate, number of invading cells and cell migration rate in CA TAM group were significantly lower than those in control group (P0.05). The colony formation rate, invasive cell number and cell migration rate in CA TAM group were significantly lower than those in CA group and TAM group (P0.05). There was no significant difference in colony formation rate, invasive cell number and cell migration rate between), CA group and TAM group (P0.05). There was no significant difference in the expression of cleaved Caspase-8 protein among two breast cancer cell lines, control group, CA group, TAM group and, CA TAM group (P0.05 in), CA group, cleaved Caspase-3, in, CA TAM group in TAM group). The expression of cleaved PARP protein was significantly higher in), CA TAM group than in control group (P0.05). The expression of cleaved Caspase-3,cleaved PARP protein in), CA TAM group was significantly higher than that in CA group and TAM group (P0.05 in), CA group and TAM group). There was no significant difference in the expression of cleaved PARP protein (P0.05). Conclusion Salmonic acid combined with tamoxifen can inhibit the proliferation, migration and invasion of breast cancer cells, and its mechanism may be related to the activation of Caspase-3/PARP signaling pathway.
【作者单位】：华中科技大学同济医学院附属武汉市中心医院中西医结合肿瘤科;
【分类号】：R737.9

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