基于阻抗谱的脉冲电场诱导兔肝脏组织不可逆电穿孔程度评估新方法的实验研究
发布时间：2018-12-25 10:10
【摘要】：不可逆电穿孔肿瘤消融的有效性与安全性已经过多年的临床应用而得到验证,新近发展起来的高频不可逆电穿孔肿瘤消融新方法兼具传统不可逆电穿孔的优势和缓解肌肉收缩的长处,然而目前对其作用机理还未深入研究。为此,从阻抗谱分析角度分析了高频不可逆电穿孔与传统不可逆电穿孔组织消融的差异性,研究了相同剂量下高频不可逆电穿孔与传统不可逆电穿孔消融新西兰大白兔肝脏组织引起的组织阻抗谱变化,并通过等效电路的拟合提取了阻抗谱变化中的信息。研究结果表明:脉冲处理后组织阻抗谱显著变化,阻抗模在中低频段显著减小,阻抗角在中高频段从负值向零值方向变化;通过等效电路拟合发现,处理后显著变化的是细胞外液等效电阻,由于细胞膜穿孔而增加了新的与细胞外液等效电阻并联的电流通路;脉冲剂量相同条件下,传统不可逆电穿孔脉冲作用引起的细胞外液等效电阻变化最大,高频不可逆电穿孔作用下,脉冲宽度越宽,细胞外液等效电阻变化就越显著。因此,组织阻抗谱变化及由它得到的等效电路元器件值变化,可以有效地反映电穿孔程度,有望成为临床应用中检测不可逆电穿孔治疗效果的有效方式。
[Abstract]:The efficacy and safety of irreversible electroporation ablation have been demonstrated in clinical practice for many years. Recently, the new ablation method of high frequency irreversible electroporation (HF irreversibly electroporation) has both the advantages of traditional irreversible electroporation and the advantages of relieving muscle contraction. However, the mechanism of its action has not been thoroughly studied. Therefore, the difference of tissue ablation between high frequency irreversible electroporation and traditional irreversible electroporation was analyzed from the point of view of impedance spectrum analysis. The changes of tissue impedance spectrum induced by high frequency irreversible electroporation and traditional irreversible electroporation ablation in New Zealand white rabbit liver were studied at the same dose. The information of impedance spectrum change was extracted by fitting the equivalent circuit. The results show that after pulse treatment, the tissue impedance spectrum changes significantly, the impedance mode decreases significantly in the middle and low frequency band, and the impedance angle changes from negative to zero in the medium and high frequency range. Through the equivalent circuit fitting, it was found that the extracellular fluid equivalent resistance changed significantly after treatment, and a new current path parallel to the extracellular fluid equivalent resistance was added due to the membrane perforation. Under the same pulse dose, the effect of the traditional irreversible electroporation pulse on the extracellular fluid equivalent resistance is the largest. The wider the pulse width is, the more obvious the change of the extracellular fluid equivalent resistance will be under the action of high frequency irreversible electroporation. Therefore, the change of tissue impedance spectrum and the changes of equivalent circuit components can effectively reflect the degree of electroporation, which is expected to be an effective way to detect the effect of irreversible electroporation in clinical application.
【作者单位】： 重庆大学电气工程学院输配电装备及系统安全与新技术国家重点实验室;Bioelectromechanical
【基金】：国家创新研究群体基金(51321063) 重庆市杰出青年基金(cstc2014jcyjjq90001) 中央高校基本科研业务费资助(106112017CDJQJ158835)~~
【分类号】：R73-36
，
本文编号：2391038
[Abstract]:The efficacy and safety of irreversible electroporation ablation have been demonstrated in clinical practice for many years. Recently, the new ablation method of high frequency irreversible electroporation (HF irreversibly electroporation) has both the advantages of traditional irreversible electroporation and the advantages of relieving muscle contraction. However, the mechanism of its action has not been thoroughly studied. Therefore, the difference of tissue ablation between high frequency irreversible electroporation and traditional irreversible electroporation was analyzed from the point of view of impedance spectrum analysis. The changes of tissue impedance spectrum induced by high frequency irreversible electroporation and traditional irreversible electroporation ablation in New Zealand white rabbit liver were studied at the same dose. The information of impedance spectrum change was extracted by fitting the equivalent circuit. The results show that after pulse treatment, the tissue impedance spectrum changes significantly, the impedance mode decreases significantly in the middle and low frequency band, and the impedance angle changes from negative to zero in the medium and high frequency range. Through the equivalent circuit fitting, it was found that the extracellular fluid equivalent resistance changed significantly after treatment, and a new current path parallel to the extracellular fluid equivalent resistance was added due to the membrane perforation. Under the same pulse dose, the effect of the traditional irreversible electroporation pulse on the extracellular fluid equivalent resistance is the largest. The wider the pulse width is, the more obvious the change of the extracellular fluid equivalent resistance will be under the action of high frequency irreversible electroporation. Therefore, the change of tissue impedance spectrum and the changes of equivalent circuit components can effectively reflect the degree of electroporation, which is expected to be an effective way to detect the effect of irreversible electroporation in clinical application.
【作者单位】： 重庆大学电气工程学院输配电装备及系统安全与新技术国家重点实验室;Bioelectromechanical
【基金】：国家创新研究群体基金(51321063) 重庆市杰出青年基金(cstc2014jcyjjq90001) 中央高校基本科研业务费资助(106112017CDJQJ158835)~~
【分类号】：R73-36
，
本文编号：2391038
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