CFTR对TF1细胞活力和凋亡的影响及相关机制

发布时间：2018-12-28 07:14

【摘要】：目的:探讨囊性纤维化跨膜传导调节因子(CFTR)在急性髓系白血病中的表达情况并探讨其对人红白血病细胞株TF1生物学功能的影响及潜在作用机制。方法:Real-time PCR技术检测急性髓系白血病患儿骨髓单个核细胞中CFTR的表达水平。常规培养的TF1细胞中加入CFTR特异性抑制剂CFTRinh-172,然后分别采用CCK-8法和流式细胞术检测细胞活力、细胞周期及细胞凋亡情况;Western blot检测Wnt信号通路相关蛋白的表达水平。结果:CFTR在急性髓系白血病患者及白血病细胞中均呈高表达。TF1细胞中加入CFTR特异性抑制剂后,细胞的活力下降,G_0/G_1期细胞比例显著升高而S期细胞比例降低,细胞凋亡率显著增加,细胞中β-catenin、c-Myc及cyclin D1的蛋白表达水平显著降低(P0.05)。结论:CFTR在急性髓系白血病中表达显著升高;抑制CFTR可通过经典的Wnt信号通路抑制白血病细胞株TF1的生长,并促进其凋亡。
[Abstract]:Aim: to investigate the expression of cystic fibrosis transmembrane conductive regulatory factor (CFTR) in acute myeloid leukemia (AML) and its effect on the biological function of human erythroleukemia cell line TF1 and its potential mechanism. Methods: Real-time PCR technique was used to detect the expression of CFTR in bone marrow mononuclear cells of children with acute myeloid leukemia. CFTRinh-172, a specific inhibitor of CFTR, was added to conventional cultured TF1 cells. Cell viability was detected by CCK-8 assay and flow cytometry, and; Western blot expression of Wnt signaling pathway proteins was detected by cell cycle and apoptosis. Results: CFTR was highly expressed in both acute myeloid leukemia patients and leukemic cells. After the addition of CFTR specific inhibitor in TF1 cells, the cell viability decreased, the proportion of G_0/G_1 phase cells increased significantly, and the percentage of S phase cells decreased. The expression of 尾-catenin,c-Myc and cyclin D1 protein decreased significantly (P0.05). Conclusion: the expression of CFTR in acute myeloid leukemia is significantly increased, and the inhibition of CFTR can inhibit the growth of leukemia cell line TF1 and promote its apoptosis through the classical Wnt signaling pathway.
【作者单位】：延边大学附属医院儿科;
【分类号】：R733.7

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