亚硒酸钠与白藜芦醇联合应用对肺癌细胞的协同效应分析

发布时间：2019-01-01 15:10

【摘要】：目的:观察亚硒酸钠与白藜芦醇单独及联合应用对肺癌细胞的影响,并通过细胞周期探讨其作用机理。方法:用四甲基偶氮唑盐法检测亚硒酸钠和白藜芦醇单独及联合应用对细胞增殖和存活的影响;用流式细胞术测定细胞的凋亡率及细胞周期。结果:亚硒酸钠和白藜芦醇对肺癌细胞A549均有拮抗作用,且随浓度的增加,拮抗效果越显著,当亚硒酸钠浓度大于等于5μmol/L时,拮抗效果极显著(P0.01);当白藜芦醇浓度大于等于65μmol/L时,拮抗效果极显著(P0.01)。亚硒酸钠对A549细胞的半致死率(lethal dose of 50%,LD_(50))为4.759μmol/L,白藜芦醇对A549细胞的LD_(50)为45.24μmol/L。然而亚硒酸钠和白藜芦醇联合用药作用于A549细胞的拮抗效果优于单独用药,且低剂量的亚硒酸钠和白藜芦醇联合就能发挥出高剂量亚硒酸钠对A549细胞的拮抗作用效果,有效地降低了亚硒酸钠对细胞的毒副作用。另外,亚硒酸钠联合白藜芦醇作用可以阻滞A549细胞G0/G1期,最终导致细胞凋亡。
[Abstract]:Aim: to observe the effect of sodium selenite and resveratrol on lung cancer cells and to explore its mechanism by cell cycle. Methods: the effects of sodium selenite and resveratrol on cell proliferation and survival were detected by tetramethylazolium salt method, and the apoptosis rate and cell cycle were measured by flow cytometry. Results: both sodium selenite and resveratrol had antagonistic effects on lung cancer cell line A549, and the antagonistic effect increased with the increase of concentration. When the concentration of sodium selenite was more than or equal to 5 渭 mol/L, the antagonistic effect was extremely significant (P0.01). When the concentration of resveratrol was greater than 65 渭 mol/L, the antagonistic effect was very significant (P0.01). The semi-lethal rate of sodium selenite on A549 cell line (lethal dose of _ (50) was 4.759 渭 mol/L, LD_ (50) of resveratrol on A549 cell line, and LD_ (50) was 45.24 渭 mol/L.. However, the antagonistic effect of sodium selenite combined with resveratrol on A549 cells was better than that on A549 cells, and low dose sodium selenite combined with resveratrol could exert the antagonistic effect of high dose sodium selenite on A549 cells. It can effectively reduce the toxicity and side effect of sodium selenite on cells. In addition, sodium selenite combined with resveratrol could block the G0/G1 phase of A549 cells and eventually lead to apoptosis.
【作者单位】：北京联合大学生物化学工程学院;
【基金】：北京市教委面上项目(KM201711417008) 北京联合大学“启明星”大学生科技创新项目
【分类号】：R734.2

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