化疗药物对Jurkat细胞表达干扰素调节因子4和P糖蛋白的影响

发布时间：2019-01-05 06:25

【摘要】：目的:探讨干扰素调节因子4在Jurkat细胞中的表达及阿霉素、硼替佐米对干扰素调节因子4、P糖蛋白表达的影响。方法:1)采用CCK8法分别检测硼替佐米、阿霉素对Jurkat细胞体外增殖的影响;2)采用RT-PCR分别检测不同时间硼替佐米、阿霉素及两药联合对Jurkat细胞IRF4m RNA及P-gp m RNA表达的影响;3)采用Western Blot检测不同时间硼替佐米、阿霉素及两药联合对Jurkat细胞IRF4蛋白表达的影响。结果:1、与对照组相比,阿霉素对Jurkat细胞的增殖有抑制作用,当阿霉素浓度为400ug/L时抑制作用最强,而且随着作用时间的延长,抑制作用逐渐增强,表现为浓度依赖性及时间依赖性(P0.05);硼替佐米对Jurkat细胞的增殖亦有抑制作用,呈时间依赖性(P0.05)。2、与对照组相比,阿霉素组IRF4 m RNA表达无明显改变,差异无统计学意义(P0.05);硼替佐米组48小时出现IRF4 m RNA表达下调(0.66±0.10),并且在72小时最明显(0.34±0.09);两药联合应用48小时出现IRF4 m RNA表达下调。3、与对照组相比,阿霉素组P-gp m RNA表达无明显改变,差异均无统计学意义(P0.05);硼替佐米组24小时P-gp m RNA表达较对照组升高,48小时、72小时出现P-gp m RNA表达下调(分别为0.79±0.02、0.59±0.00);两药联合48小时后出现P-gp m RNA表达下调(0.73±0.00),以72小时最明显(0.47±0.00)。4、各用药时间段阿霉素对IRF4蛋白的表达均未产生明显影响(P0.05);硼替佐米组48小时、72小时出现IRF4蛋白表达下调(P0.05);两药联合组48小时出现IRF4蛋白表达的下调,以72小时最明显(P0.05)。结论:1、干扰素调节因子4在Jurkat细胞中阳性表达;2、阿霉素能抑制Jurkat细胞增殖,但不能抑制干扰素调节因子4和P糖蛋白的表达;3、硼替佐米对Jurkat细胞增殖有抑制作用,其机制可能是通过下调干扰素调节因子4和P糖蛋白的表达。
[Abstract]:Aim: to investigate the expression of interferon regulatory factor 4 in Jurkat cells and the effects of doxorubicin and bortezomib on the expression of interferon regulatory factor 4 P glycoprotein. Methods: 1) the effects of bortezomil and doxorubicin on the proliferation of Jurkat cells were detected by CCK8 assay in vitro. 2) the effects of bortezomil, adriamycin and two drugs on the expression of IRF4m RNA and P-gp m RNA in Jurkat cells were detected by RT-PCR. 3) the effects of bortezomil, adriamycin and two drugs on the expression of IRF4 protein in Jurkat cells were detected by Western Blot. Results: 1. Compared with the control group, doxorubicin inhibited the proliferation of Jurkat cells. When the concentration of doxorubicin was 400ug/L, the inhibitory effect was the strongest, and the inhibitory effect increased gradually with the prolongation of the action time. It showed concentration dependence and time dependence (P0.05). Bortezomil also inhibited the proliferation of Jurkat cells in a time-dependent manner (P0.05). Compared with the control group, the expression of IRF4 m RNA in adriamycin group had no significant change (P0.05). The expression of IRF4 m RNA was down-regulated (0.66 卤0.10) at 48 hours in bortezomil group, and was most obvious at 72 hours (0.34 卤0.09). The expression of IRF4 m RNA was down-regulated at 48 hours after treatment with adriamycin. Compared with the control group, the expression of P-gp m RNA in adriamycin group had no significant change (P0.05). Compared with the control group, the expression of P-gp m RNA in bortezomil group increased at 24 hours, and decreased at 48 hours and 72 hours (0.79 卤0.02 卤0.59 卤0.00, respectively). The expression of P-gp m RNA was down-regulated (0.73 卤0.00) 48 hours after the combination of the two drugs, especially at 72 hours (0.47 卤0.00). The expression of IRF4 protein was not significantly affected by adriamycin (P0.05). The expression of IRF4 protein was down-regulated at 48h and 72h in bortezomib group (P0.05), and the down-regulation of IRF4 protein was observed at 48h in combination group, the most obvious at 72h (P0.05). Conclusion: 1.Interferon regulatory factor 4 is positive in Jurkat cells, adriamycin can inhibit the proliferation of Jurkat cells, but it can not inhibit the expression of interferon regulatory factor 4 and P glycoprotein. 3Bortezomil inhibited the proliferation of Jurkat cells by down-regulating the expression of interferon regulatory factor 4 and P-glycoprotein.
【学位授予单位】：遵义医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R733.71

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