白细胞介素-17与肝癌家族聚集性的关系
发布时间：2019-01-07 16:07
【摘要】：目的研究白细胞介素-17在广西肝癌高发地区肝癌高发家族成员与无癌家族成员血清中的表达情况,探讨IL-17的表达水平与广西肝癌家族聚集性的相关性。方法在广西肝癌高发地区配对选取112对肝癌高发家族和无癌家族成员,共224例,高发家族组有男65例,女47例,年龄(27.1±21.2)岁,无癌家族组有男65例,女47例,年龄(26.8±17.7)岁。112例高发家族成员中根据亲缘关系的不同,分一级亲属组、二级亲属组、三级亲属组、四级及以上亲属组,其中一级亲属62例,二级亲属11例,三级亲属25例,四级及以上亲属14例;根据高发家族组中患肝癌例数的不同,分2~3例组,4例及以上两组,其中2~3例组70例,4例及以上组42例。以高发家族成员为实验组,无癌家族成员为对照组,采用酶联免疫吸附(Enzyme-Linked Immunosorbent Assay,ELISA)法检测两组研究对象外周血清IL-17的表达水平。结果(1)高发家族组、无癌家族组IL-17的总体表达水平分别为(71.13±30.22)pg/ml、(19.01±14.46)pg/ml,高发家族成员IL-17表达水平显著高于无癌家族成员,差异有统计学意义(t=16.464,P=0.000);进一步分层分析:高发家族组男性、女性、年龄≤25岁、年龄25岁、HBs Ag阳性、HBs Ag阴性、壮族、瑶族成员IL-17的平均浓度分别为(71.16±28.43)pg/ml、(71.08±32.86)pg/ml、(69.32±29.53)pg/ml、(73.73±31.33)pg/ml、(70.14±20.11)pg/ml、(71.82±35.76)pg/ml、(72.38±33.65)pg/ml、(67.99±19.25)pg/ml,无癌家族组男性、女性、年龄≤25岁、年龄25岁、HBs Ag阳性、HBs Ag阴性、壮族、瑶族成员IL-17的平均浓度分别为(17.36±14.45)pg/ml、(21.30±14.30)pg/ml、(17.19±13.33)pg/ml、(21.63±15.71)pg/ml、(18.48±13.99)pg/ml、(19.39±14.87)pg/ml、(19.25±15.09)pg/ml、(18.41±12.93)pg/ml,两组比较,t值分别为13.603、9.524、13.072、10.081、14.307、10.999、12.886、12.094,所有的P值均0.05,高发家族组不同人口学特征IL-17表达水平都高于无癌家族组,差异有统计学意义。(2)高发家族组中不同亲缘系数的成员之间IL-17表达水平差异无统计学意义(Z=4.831,P=0.185)。(3)高发家族组中随着患肝癌例数增加,IL-17表达水平下降,但差异无统计学意义(t=0.580,P=0.563)。结论肝癌高发家族成员IL-17表达水平升高与肝癌家族聚集现象存在相关性。
[Abstract]:Objective to study the expression of interleukin-17 (IL-17) in the serum of high incidence family members and non-cancerous family members of hepatocellular carcinoma (HCC) in Guangxi, and to explore the correlation between the expression of IL-17 and the aggregation of HCC family in Guangxi. Methods A total of 224 members of 112 high incidence and no cancer families in Guangxi were selected. There were 65 males and 47 females, aged (27.1 卤21.2) years. There were 65 males and 47 females in the cancer free family group. Age (26.8 卤17.7). 112 family members with high incidence were divided into first-degree relatives group, second-degree relatives group, third-degree relatives group, and four-grade relatives group, among which 62 cases were first-degree relatives and 11 cases second-degree relatives. 25 cases of third-degree relatives, 14 cases of four-grade and above relatives; According to the number of HCC cases in the high incidence family group, we divided them into 2 groups: 3 cases, 4 cases and more, including 70 cases in 23 cases, 42 cases in 4 cases and above. The expression of IL-17 in peripheral blood of the two groups was detected by enzyme linked immunosorbent assay (Enzyme-Linked Immunosorbent Assay,ELISA) with high incidence family members as experimental group and non cancerous family members as control group. Results (1) the total expression level of IL-17 was (71.13 卤30.22) pg/ml, (19.01 卤14.46) pg/ml, and (19.01 卤14.46) pg/ml, in high incidence family and cancer free family respectively. The difference was statistically significant (t = 16.464, P = 0.000). Further stratification analysis: male, female, age 鈮，
本文编号：2403854
[Abstract]:Objective to study the expression of interleukin-17 (IL-17) in the serum of high incidence family members and non-cancerous family members of hepatocellular carcinoma (HCC) in Guangxi, and to explore the correlation between the expression of IL-17 and the aggregation of HCC family in Guangxi. Methods A total of 224 members of 112 high incidence and no cancer families in Guangxi were selected. There were 65 males and 47 females, aged (27.1 卤21.2) years. There were 65 males and 47 females in the cancer free family group. Age (26.8 卤17.7). 112 family members with high incidence were divided into first-degree relatives group, second-degree relatives group, third-degree relatives group, and four-grade relatives group, among which 62 cases were first-degree relatives and 11 cases second-degree relatives. 25 cases of third-degree relatives, 14 cases of four-grade and above relatives; According to the number of HCC cases in the high incidence family group, we divided them into 2 groups: 3 cases, 4 cases and more, including 70 cases in 23 cases, 42 cases in 4 cases and above. The expression of IL-17 in peripheral blood of the two groups was detected by enzyme linked immunosorbent assay (Enzyme-Linked Immunosorbent Assay,ELISA) with high incidence family members as experimental group and non cancerous family members as control group. Results (1) the total expression level of IL-17 was (71.13 卤30.22) pg/ml, (19.01 卤14.46) pg/ml, and (19.01 卤14.46) pg/ml, in high incidence family and cancer free family respectively. The difference was statistically significant (t = 16.464, P = 0.000). Further stratification analysis: male, female, age 鈮，
本文编号：2403854
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