沉默HERC4表达可抑制宫颈癌细胞的增殖、凋亡和迁移
发布时间：2019-01-11 08:10
【摘要】：目的探讨HERC4对宫颈癌细胞生物学功能的影响及其分子机制。方法设计特异HERC4 si RNA干扰序列,Lipofectamine2000法转染Hela细胞,Western blotting检测转染特异si RNA后Hela细胞HERC4蛋白的表达水平;CCK-8法检测转染后Hela细胞的增殖能力;AnnexinⅤ-FITC/PI双染法检测转染后Hela细胞凋亡率的变化;划痕实验检测转染后Hela细胞迁移能力。Western blotting检测转染后Hela细胞Cyclin D1、Bcl-2表达变化。结果转染特异si RNA后Hela细胞HERC4蛋白表达量明显下降,差异有统计学意义(P0.01)。与对照组相比,干扰组Hela细胞生长速度明显减慢,细胞凋亡率增加,迁移能力明显下降,差异有统计学意义(P0.01)。转染后Cyclin D1和Bcl-2在蛋白水平的表达显著下调(P0.01)。结论 si RNA可有效降低宫颈癌Hela细胞中HERC4的表达,并通过抑制Cyclin D1表达抑制宫颈癌Hela细胞的增殖和迁移能力,抑制Bcl-2表达增加细胞凋亡能力。
[Abstract]:Objective to investigate the effect of HERC4 on the biological function of cervical cancer cells and its molecular mechanism. Methods the specific HERC4 si RNA interference sequence was designed and the expression level of HERC4 protein in Hela cells after transfection of specific si RNA was detected by, Western blotting with Lipofectamine2000 method, and the proliferation ability of Hela cells was detected by CCK-8 method. The apoptosis rate of Hela cells after transfection was detected by Annexin 鈪，
本文编号：2406892
[Abstract]:Objective to investigate the effect of HERC4 on the biological function of cervical cancer cells and its molecular mechanism. Methods the specific HERC4 si RNA interference sequence was designed and the expression level of HERC4 protein in Hela cells after transfection of specific si RNA was detected by, Western blotting with Lipofectamine2000 method, and the proliferation ability of Hela cells was detected by CCK-8 method. The apoptosis rate of Hela cells after transfection was detected by Annexin 鈪，
本文编号：2406892
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