急性早幼粒细胞白血病的细胞表面免疫分子的检测和分析

发布时间：2019-01-14 12:35

【摘要】：目的研究急性早幼粒细胞白血病(APL)表型特征及临床意义。方法采用流式细胞术四色方案对APL进行免疫分型,以CD45/SSC-lin图定位白血病细胞,依据抗原表达强度将抗原表达划分为五级分析。结果典型APL细胞抗原表达强度及所占比例依次为:CD33(100%)、髓过氧化物酶(MPO)100%、CD38(82.35%)、CD13(64.71%)、CD64(50%)、CD123(47.06)、CD117(44.12%),HLA-DR和CD34基本不表达;伴淋系表达APL患者化疗后缓解率明显低于阴性APL患者。结论 APL免疫分型典型表型特征为高侧向角散射(high SSC)、CD33~+(Ⅰ级)、CD38~+(Ⅰ级)、MPO~+(Ⅰ级)、CD13~+(Ⅲ级)、CD64~+(Ⅰ级/Ⅲ级)、CD117~+(Ⅱ级/Ⅲ级/Ⅳ级)、CD123~+(Ⅲ级/Ⅳ级)、CD11b~-、HLA-DR~-、CD34~-。掌握APL免疫表型特征有助于APL快速诊断,且对MRD检测有指导意义。
[Abstract]:Objective to study the phenotypic characteristics and clinical significance of (APL) in acute promyelocytic leukemia. Methods Immunophenotyping of APL was carried out by flow cytometry, and the leukemic cells were located by CD45/SSC-lin, and the antigen expression was divided into five stages according to the intensity of antigen expression. Results the antigen expression intensity and percentage of typical APL cells were as follows: CD33 (100%), myeloperoxidase (MPO) 100 (82.35%), CD13 (64.71%), CD64 (50%), CD123 (47.06), CD117 (44.12%). HLA-DR and CD34 were not expressed. The remission rate after chemotherapy in patients with lymphoid expression of APL was significantly lower than that in patients with negative APL. Conclusion the typical phenotypic features of APL immunophenotype are high lateral angle scattering (high SSC), CD33~ (鈪，

本文编号：2408699