EDIL3在膀胱癌中的表达及与VEGF相关性的研究

发布时间：2019-01-21 09:29

【摘要】：膀胱癌是一种在世界范围内泌尿系恶性肿瘤中最常见的肿瘤,排在恶性肿瘤的第11位,发病率男性患者排在第7位,在女性患者中排在第10位。在我国,男性膀胱癌发病率在全身恶性肿瘤中排第7位,多是膀胱尿路上皮癌,占所有病理类型的90%,此外还有腺细胞癌和鳞状细胞癌等。血尿是膀胱癌最常见的症状,尤其是间歇性无痛性肉眼血尿,也可能会合并尿频,尿急等膀胱刺激症状及下腹部疼痛。膀胱镜检查及活检是诊断膀胱癌最可靠的方法。根据临床不同的特征膀胱癌的治疗也不同,手术现在是膀胱癌外科主要的治疗方法。容易复发,多发是膀胱肿瘤最显著的生物学行为,浸润、转移及肿瘤血管生成几乎贯穿于膀胱癌的整个发展过程。目前,肿瘤血管密度已成为判断膀胱癌预后的独立指标,高血管密度是肿瘤复发的危险因素,膀胱癌浸润性生长依赖于血管生成,因此,探讨膀胱癌血管生成相关的分子机制,有助于开发阻断或延缓膀胱癌进展的重要分子靶向药物。目的:观察EDIL3在不同的分级、分期的膀胱移行细胞癌组织及癌旁组织中的表达状况,探索EDIL3在膀胱尿路上皮癌发生、发展中的作用及与血管内皮生长因子(VEGF)表达的关系,为膀胱癌的诊断和治疗提供帮助。方法:选用免疫组织化学PV9001二步法观察24例膀胱癌旁组织、57例膀胱尿路上皮癌组织中EDIL3基因及VEGF基因的表达状况,结合病理分级及分期进行统计并分析两个基因表达的相关性。结果:1.膀胱癌细胞中EDIL3基因阳性表达率显著高于癌旁组织,分别为43.9%(25/57),16.7%(4/24),表达有明显差异,P0.05,有统计学差异。2.EDIL3基因的表达与患者年龄、体重、发病时间、肿块体积、有无吸烟史及肿块个数均无相关性(P0.05),但与膀胱癌细胞浸润的层次深度即肿瘤的病理分期有相关性(P0.05)。3.各病理学分级高表达率为1级15.8%(3/19),2级62.5%(10/16),3级66.7%(12/18)。需注意的是肿瘤的病理分级中,EDIL3基因的表达随膀胱癌恶性级别的增高而表达强度增加,2级与1级相比具有显著统计学差异(P0.05),3级与1级相比具有显著统计学差异(P0.05),但是3级与2级的差别无统计学意义(P0.05)。4.EDIL3和VEGF的表达呈正相关(rS=0.239,P0.05),膀胱癌组织中均呈上升趋势。结论:EDIL3基因的表达与膀胱癌病理分级,分期及血管内皮生长因子相关基因的表达有相关性,可能与膀胱癌的发生、发展有重要关联。EDIL3检测可以对判断肿瘤的浸润深度和恶性程度提供一定的帮助。
[Abstract]:Bladder cancer is the most common malignant tumor of urinary system in the world, ranking 11th among malignant tumors, 7th in male patients and 10th in female patients. In China, the incidence of bladder cancer in men ranks 7th in the total malignant tumor, mostly bladder urothelial carcinoma, accounting for 90% of all pathological types, in addition to adenocarcinoma and squamous cell carcinoma and so on. Hematuria is the most common symptom of bladder cancer, especially intermittent painless hematuria. Cystoscopy and biopsy are the most reliable methods for the diagnosis of bladder cancer. Surgery is now the main surgical treatment for bladder cancer according to its clinical characteristics. It is easy to recur and multiple is the most significant biological behavior of bladder cancer. Invasion metastasis and tumor angiogenesis almost run through the development of bladder cancer. At present, tumor vascular density has become an independent index to judge the prognosis of bladder cancer. High vascular density is a risk factor for tumor recurrence. Invasive growth of bladder cancer depends on angiogenesis. It helps to develop important molecular targeting drugs that block or delay the progression of bladder cancer. Objective: to observe the expression of EDIL3 in bladder transitional cell carcinoma (TCC) and adjacent tissues in different grades and stages, and to explore the role of EDIL3 in the occurrence and development of bladder urothelial carcinoma and its relationship with the expression of vascular endothelial growth factor (VEGF) (VEGF). To provide help for the diagnosis and treatment of bladder cancer. Methods: the expression of EDIL3 gene and VEGF gene in 24 cases of bladder adjacent tissues and 57 cases of bladder urothelial carcinoma were observed by immunohistochemical PV9001 two-step method. Combined with pathological grading and staging, the correlation between the two genes expression was analyzed. Results: 1. The positive expression rate of EDIL3 gene in bladder cancer cells was significantly higher than that in adjacent tissues (43.9% (25 / 57) and 16.7% (4 / 24), respectively). There was no correlation between the expression of 2.EDIL3 gene and age, weight, onset time, mass volume, smoking history and the number of masses (P0.05). However, there was a correlation between the depth of bladder cancer cell infiltration and the pathological stage of the tumor (P0.05). The high expression rates were 15.8% (3 / 19) in grade 1, 62.5% (10 / 16) in grade 2 and 66.7% (12 / 18) in grade 3. It should be noted that the expression of EDIL3 gene increased with the increase of malignant grade of bladder cancer, and there was significant difference between grade 2 and grade 1 (P0.05). There was significant difference between grade 3 and grade 1 (P0.05), but there was no significant difference between grade 3 and grade 2 (P0.05). The expression of 4.EDIL3 and VEGF was positively correlated (rS=0.239,P0.05). There was an increasing trend in bladder cancer tissues. Conclusion: the expression of EDIL3 gene is correlated with the pathological grade, stage and VEGF-related gene expression of bladder cancer, and may be associated with the occurrence of bladder cancer. EDIL3 can be helpful in determining the depth of invasion and the degree of malignancy of the tumor.
【学位授予单位】：兰州大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R737.14

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