GANT61通过自噬性细胞死亡抑制人乳腺癌MCF-7细胞增殖
发布时间：2019-01-21 11:26
【摘要】：目的观察Hedgehog通路抑制剂GANT61对人乳腺癌MCF-7细胞的抑制作用。方法 GANT61作用MCF-7细胞后,通过流式细胞术检测细胞死亡;提取处理细胞总RNA和蛋白。分别采用Real-time PCR和Western blot检测Hedgehog通路SHH、Gli1、Gli2及自噬标志物LC3-Ⅱ表达;处理细胞固定后通过电子显微镜观察GANT61诱导的自噬体形态;自噬抑制剂3-MA处理细胞或自噬相关基因atg5 siRNA转染细胞后再用GANT61作用,流式细胞术检测细胞死亡数量。结果 GANT61能明显诱导MCF-7死亡,降低Hedgehog通路SHH、Gli1、Gli2表达水平,提高LC3-Ⅱ蛋白表达,并可诱导MCF-7细胞产生自噬体。3-MA及atg5 siRNA可减弱GANT61诱导的细胞死亡。结论 GANT61通过诱导自噬性细胞死亡机制发挥抗乳腺癌细胞活性。
[Abstract]:Objective To observe the inhibitory effect of the Hedgehog pathway inhibitor, GANT61, on human breast cancer MCF-7 cells. Methods The cell death was detected by flow cytometry, and the total RNA and protein of the cells were extracted by flow cytometry. The expression of the Hedgehog pathway SHH, Gli1, Gli2 and the self-autophagy marker LC3-鈪，
本文编号：2412616
[Abstract]:Objective To observe the inhibitory effect of the Hedgehog pathway inhibitor, GANT61, on human breast cancer MCF-7 cells. Methods The cell death was detected by flow cytometry, and the total RNA and protein of the cells were extracted by flow cytometry. The expression of the Hedgehog pathway SHH, Gli1, Gli2 and the self-autophagy marker LC3-鈪，
本文编号：2412616
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