低氧诱导因子-1α在胃癌组织中表达水平及分子作用机制研究

发布时间：2019-02-11 12:56

【摘要】：目的:检测低氧诱导因子-1α(Hypoxia inducible factor-1α,HIF-1α)在胃癌组织的表达水平,探讨HIF-1α在胃癌发病过程的分子作用机制。方法:选取世居于青海省境内河湟谷地的新发胃癌患者60例,设为高原组;选取平原地区(深圳)新发胃癌患者60例,设为平原组。收集两组患者胃癌组织标本,采用免疫组织化学染色SP法检测两组患者组织标本中HIF-1α蛋白的表达情况。采用免疫荧光染色测定两组受试者胃癌组织的HIF-1α的表达量。采用RT-PCR检测两组患者组织标本中miR-210的表达水平。分析胃癌患者HIF-1α的表达与临床病理特征的关系。结果:高原组患者胃癌组织的HIF-1α蛋白表达量显著高于平原组(P0.05)。高原组患者胃癌组织的HIF-1αmRNA含量也显著高于平原组(P0.05)。高原组患者胃癌组织的miR-210的表达水平也显著高于平原组(P0.05)。HIF-1α在胃癌中的阳性表达与肿瘤的分化(P=0.044 1)、浸润深度(P=0.031 9)、淋巴结转移(P=0.025 3)及TNM分期(P=0.028 9)相关。结论:低氧环境下胃癌组织中HIF-1α水平显著增加,并且HIF-1α能通过miR-210促进胃癌细胞进展。
[Abstract]:Aim: to detect the expression of hypoxia inducible factor-1 伪 (Hypoxia inducible factor-1 伪 (HIF-1 伪) in gastric carcinoma and to explore the molecular mechanism of HIF-1 伪 in the pathogenesis of gastric cancer. Methods: sixty newly developed gastric cancer patients living in Hehuang valley in Qinghai province were selected as plateau group and 60 new gastric cancer patients in plain area (Shenzhen) as plain group. The expression of HIF-1 伪 protein was detected by immunohistochemical staining SP method. The expression of HIF-1 伪 in gastric cancer tissues was determined by immunofluorescence staining. RT-PCR was used to detect the expression of miR-210 in the tissues of the two groups. To analyze the relationship between the expression of HIF-1 伪 and clinicopathological features in patients with gastric cancer. Results: the expression of HIF-1 伪 protein in high altitude group was significantly higher than that in plain group (P 0.05). The content of HIF-1 伪 mRNA in gastric cancer tissue in high altitude group was significantly higher than that in plain group (P 0.05). The expression level of miR-210 in gastric cancer tissue of high altitude group was also significantly higher than that in plain group (P0.05). The positive expression of HIF-1 伪 in gastric cancer and tumor differentiation (P0. 044 1), depth of invasion (P0. 031 9), the expression of HIF-1 伪 in gastric carcinoma were also significantly higher than those in plain group (P0.05). Lymph node metastasis (P0. 025 3) and TNM stage (P0. 028 9) were correlated. Conclusion: the level of HIF-1 伪 in gastric cancer tissues was significantly increased under hypoxia, and HIF-1 伪 could promote the progression of gastric cancer cells through miR-210.
【作者单位】：青海大学附属医院胃肠肿瘤外科;
【分类号】：R735.2

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