胃癌组织中多效蛋白的表达及其临床意义
发布时间：2019-02-14 20:34
【摘要】：目的:研究多效蛋白(pleiotrophin,PTN)在胃癌组织及胃癌患者外周血中的表达,进一步探讨PTN在胃癌发病过程的作用以及其在胃癌诊断、治疗策略制定和预后判断作用。方法:1.收集在我院于2015年03月 2016年12月行胃癌根治手术患者70例(设为实验组),在我院行健康体检者30例(设为对照组)。确立纳入标准以及排除标准:纳入标准:(1)术前通过胃镜和病理检查明确诊断为胃癌患者;(2)拟行胃癌根治手术者;(3)心、肺等重要的脏器功能良好,能够承受胃癌手术打击;(4)知晓实验方法和实验目的,自愿参加并配合。排除标准:(1)胃癌术后出现复发、残胃癌、合并其他肿瘤者;(2)既往有胃肠道手术史或胃溃疡、肠炎病史者;(3)伴有心肺等重要脏器功能不全者;(4)有造血系统疾病者;(5)近期使用免疫抑制剂、激素类药物或血液制品者;(6)合并有严重营养不良者;(7)术前行放化疗、免疫治疗及或其他相关治疗者。2.术中取患者胃癌组织及癌旁正常组织(距肿瘤组织5cm),通过免疫组织化学方法测定其PTN表达,并分析PTN表达与胃癌临床病理特征之间的关系。3.入院后抽取外周血,通过ELISA法测定血液中PTN的表达水平,通过比较胃癌患者与健康体检者外周血中PTN表达差异,分析其与肿瘤TNM分期、肿瘤分型等临床病理特征之间的关系。结果:1.胃癌组织中PTN阳性表达率(81.4%)明显高于远癌组织(11.4%),组间差异具有统计学意义(P0.05)。胃癌组织中PTN的表达:胃癌组织直径≥4cm者的PTN表达高于胃癌组织直径4cm(P0.05);TNM分期中Ⅲ-Ⅳ期患者高于Ⅰ-Ⅱ期患者(P0.05);中分化+高分化胃癌患者低于未分化+低分化胃癌患者(P0.05);Borrmann分型的0-Ⅱ型胃癌患者低于Ⅲ-Ⅳ型胃癌患者(P0.05);有淋巴结转移组高于无淋巴结转移组(P0.05)。PTN在胃癌组织中的表达与年龄和性别无关(P0.05)。2.胃癌患者外周血中PTN浓度为(54.739±20.300)ng/ml,明显高于体检组(5.041±2.255)ng/ml,差异有统计学意义(P0.05)。PTN在胃癌患者外周血中的表达:胃癌组织直径≥4cm者的PTN表达高于胃癌组织直径4cm(P0.05);TNM分期中Ⅲ-Ⅳ期患者高于Ⅰ-Ⅱ期患者(P0.05);有淋巴结转移组高于无淋巴结转移组(P0.05)。胃癌患者外周血中PTN浓度与性别、年龄和分化程度无关(P0.05)。结论:1.胃癌组织中PTN表达水平较远癌组织明显升高,判断手术切缘阴阳性及微转移。2.胃癌患者外周血PTN表达水平高于健康体检者的表达水平,与胃癌患者肿瘤大小、TNM分期、淋巴结转移等临床病理学特征有关,有望成为胃癌诊断的检验学指标。3.PTN在胃癌组织中的表达与肿瘤分期、分型及分化程度相关,提示PTN可为胃癌诊断、治疗策略制定以及预后提供参考。4.PTN能为胃癌的筛查及诊断提供依据。
[Abstract]:Objective: to investigate the expression of multifunctional protein (pleiotrophin,PTN) in gastric cancer tissues and peripheral blood of patients with gastric cancer, and to explore the role of PTN in the pathogenesis of gastric cancer and in the diagnosis, treatment strategy and prognosis of gastric cancer. Methods: 1. A total of 70 patients (experimental group) and 30 healthy persons (control group) underwent radical gastrectomy in our hospital from March 2015 to December 2016 were collected. Establishment of inclusion criteria and exclusion criteria: (1) preoperative diagnosis of gastric cancer by gastroscopy and pathological examination; (2) preparation of radical surgery for gastric cancer; (3) the important organs such as heart and lung have good function and can withstand the attack of gastric cancer surgery. (4) knowing the experimental method and purpose, volunteer to participate and cooperate. Exclusion criteria: (1) recurrence of gastric cancer after operation, residual gastric cancer, complicated with other tumors; (2) previous gastrointestinal surgery or gastric ulcer, enteritis history; (3) patients with heart and lung dysfunction; (4) hematopoietic diseases; (5) recent use of immunosuppressants, hormone drugs or blood products; (6) severe malnutrition; (7) preoperative radiotherapy, chemotherapy, immunotherapy and other related treatments. 2. The expression of PTN in gastric cancer tissues and adjacent normal tissues (5cm) was determined by immunohistochemical method. The relationship between PTN expression and clinicopathological features of gastric cancer was analyzed. 3. The expression of PTN in peripheral blood was measured by ELISA method after admission. The expression of PTN in peripheral blood of patients with gastric cancer was compared with that of healthy controls, and the relationship between PTN expression and clinicopathological features such as tumor TNM staging and tumor classification was analyzed. Results: 1. The positive expression rate of PTN in gastric cancer (81.4%) was significantly higher than that in distant carcinoma (11.4%), and the difference was statistically significant (P0.05). The expression of PTN in gastric cancer: the expression of PTN in gastric cancer with diameter 鈮，
本文编号：2422583
[Abstract]:Objective: to investigate the expression of multifunctional protein (pleiotrophin,PTN) in gastric cancer tissues and peripheral blood of patients with gastric cancer, and to explore the role of PTN in the pathogenesis of gastric cancer and in the diagnosis, treatment strategy and prognosis of gastric cancer. Methods: 1. A total of 70 patients (experimental group) and 30 healthy persons (control group) underwent radical gastrectomy in our hospital from March 2015 to December 2016 were collected. Establishment of inclusion criteria and exclusion criteria: (1) preoperative diagnosis of gastric cancer by gastroscopy and pathological examination; (2) preparation of radical surgery for gastric cancer; (3) the important organs such as heart and lung have good function and can withstand the attack of gastric cancer surgery. (4) knowing the experimental method and purpose, volunteer to participate and cooperate. Exclusion criteria: (1) recurrence of gastric cancer after operation, residual gastric cancer, complicated with other tumors; (2) previous gastrointestinal surgery or gastric ulcer, enteritis history; (3) patients with heart and lung dysfunction; (4) hematopoietic diseases; (5) recent use of immunosuppressants, hormone drugs or blood products; (6) severe malnutrition; (7) preoperative radiotherapy, chemotherapy, immunotherapy and other related treatments. 2. The expression of PTN in gastric cancer tissues and adjacent normal tissues (5cm) was determined by immunohistochemical method. The relationship between PTN expression and clinicopathological features of gastric cancer was analyzed. 3. The expression of PTN in peripheral blood was measured by ELISA method after admission. The expression of PTN in peripheral blood of patients with gastric cancer was compared with that of healthy controls, and the relationship between PTN expression and clinicopathological features such as tumor TNM staging and tumor classification was analyzed. Results: 1. The positive expression rate of PTN in gastric cancer (81.4%) was significantly higher than that in distant carcinoma (11.4%), and the difference was statistically significant (P0.05). The expression of PTN in gastric cancer: the expression of PTN in gastric cancer with diameter 鈮，
本文编号：2422583
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