MicroRNA-27a在青海藏族胃癌组织中的表达和临床病理关系的相关分析
发布时间：2019-02-15 17:51
【摘要】：目的:检测青海地区藏族胃癌组织与癌周边组织中miR-27a的表达水平,比较miR-27a在两者不同组织中的表达水平是否具有差异;分析胃癌组织中miR-27a的相对定量和临床相关病理资料是否具有相关性;分析从而探讨miR-27a在青海藏族胃癌组织中的表达意义;并与其他民族胃癌组织中的表达情况进行对比分析。方法:收集藏族胃癌病理组织标本50例,分别将胃癌组织及癌旁组织取材并包埋成病理组织石蜡蜡块,从石蜡蜡块标本内提取miRNA、进行逆转录并采取RT-PCR方法检测胃癌组织和癌周边组织两者中mi R-27a的相对定量。并同时收集汇总藏族胃癌患者的临床相关病理资料,并结合检测结果进行统计分析。检索其他民族相关文献中关于胃癌组织中miR-27a的报道,进行对比探讨。结果:(1)MiR-27a在藏族胃癌组织中的表达水平为7.415±0.129,在藏族癌旁组织的表达水平为2.050±0.062,两组之间的表达差异具有统计学的意义(P0.05),即miR-27a在青海藏族胃癌组织中的表达高于其癌旁组织。(2)Mi R-27a在淋巴结发生癌转移的胃癌组织中的表达水平高于未发生淋巴结癌转移者,两者间的差异具有统计学意义(P0.05),而miR-27a的表达水平与其他临床相关的病理因素如诊断时年龄、患者的性别及其胃癌肿瘤组织分化程度无显著相关性。(3)早期胃癌患者(Ⅰ-Ⅱ期)与晚期胃癌患者(Ⅲ-Ⅳ期)的癌组织中miR-27a的表达水平未见明显差异。结论:(1)藏族胃癌组织中miR-27a的表达水平上调,并且其相对定量与淋巴结是否癌转移具有一定相关性,提示miR-27a对疾病的诊断有一定提示意义,并可推测胃癌的进展及预后情况。(2)藏族胃癌早期与晚期患者中mi R-27a的表达量未见明显差异,推测miR-27a可能参与胃癌早期启动的发生过程。(3)藏族胃癌组织中miR-27a的表达情况与其他民族文献记录相一致。
[Abstract]:Objective: to detect the expression level of miR-27a in Tibetan gastric cancer tissues and surrounding tissues in Qinghai, and to compare the expression level of miR-27a in different tissues. To analyze the correlation between the relative quantitative and clinicopathological data of miR-27a in gastric cancer tissues, and to explore the significance of miR-27a expression in Tibetan gastric cancer tissues of Qinghai province. The expression of gastric cancer in other nationalities was compared with that in other ethnic groups. Methods: 50 specimens of gastric cancer were collected, and the samples of gastric cancer tissues and adjacent tissues were collected and embedded into paraffin wax blocks of pathological tissues, and miRNA, was extracted from paraffin wax samples. Reverse transcription and RT-PCR method were used to detect the relative quantification of mi R-27a in both gastric cancer tissues and surrounding tissues. At the same time, the clinicopathological data of Tibetan patients with gastric cancer were collected and analyzed statistically. To search for the reports of miR-27a in gastric cancer tissues from other nationalities, and to make a comparative study. Results: (1) the expression level of MiR-27a was 7.415 卤0.129 in Tibetan gastric cancer and 2.050 卤0.062 in adjacent tissues of Tibetan cancer. The difference between the two groups was statistically significant (P0.05). That is, the expression of miR-27a in Qinghai Tibetan gastric cancer was higher than that in adjacent tissues. (2) the expression of Mi R-27a was higher in gastric cancer tissues with lymph node metastasis than in those without lymph node metastasis. The difference between the two groups was statistically significant (P0.05), and the expression of miR-27a was correlated with other clinicopathological factors such as age at the time of diagnosis. (3) there was no significant difference in the expression of miR-27a between patients with early gastric cancer (stage 鈪，
本文编号：2423583
[Abstract]:Objective: to detect the expression level of miR-27a in Tibetan gastric cancer tissues and surrounding tissues in Qinghai, and to compare the expression level of miR-27a in different tissues. To analyze the correlation between the relative quantitative and clinicopathological data of miR-27a in gastric cancer tissues, and to explore the significance of miR-27a expression in Tibetan gastric cancer tissues of Qinghai province. The expression of gastric cancer in other nationalities was compared with that in other ethnic groups. Methods: 50 specimens of gastric cancer were collected, and the samples of gastric cancer tissues and adjacent tissues were collected and embedded into paraffin wax blocks of pathological tissues, and miRNA, was extracted from paraffin wax samples. Reverse transcription and RT-PCR method were used to detect the relative quantification of mi R-27a in both gastric cancer tissues and surrounding tissues. At the same time, the clinicopathological data of Tibetan patients with gastric cancer were collected and analyzed statistically. To search for the reports of miR-27a in gastric cancer tissues from other nationalities, and to make a comparative study. Results: (1) the expression level of MiR-27a was 7.415 卤0.129 in Tibetan gastric cancer and 2.050 卤0.062 in adjacent tissues of Tibetan cancer. The difference between the two groups was statistically significant (P0.05). That is, the expression of miR-27a in Qinghai Tibetan gastric cancer was higher than that in adjacent tissues. (2) the expression of Mi R-27a was higher in gastric cancer tissues with lymph node metastasis than in those without lymph node metastasis. The difference between the two groups was statistically significant (P0.05), and the expression of miR-27a was correlated with other clinicopathological factors such as age at the time of diagnosis. (3) there was no significant difference in the expression of miR-27a between patients with early gastric cancer (stage 鈪，
本文编号：2423583
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