CFTR与E-cadherin蛋白在结直肠癌中的表达及临床意义
发布时间：2019-02-16 00:13
【摘要】：结直肠癌是一种常见的消化道恶性肿瘤疾病,在我们国家,结直肠癌的发病率和死亡率均高于世界平均水平。由于临床常常确诊较晚,治疗费用较高,造成了严重的家庭经济负担和社会负担。结直肠癌的发病机制和临床治疗是目前研究的热点和难点。本实验通过研究囊性纤维化跨膜传导调节因子(cystic fibrosis transmembrane conductance regulator CFTR)与E-cadherin蛋白在结直肠癌组织和正常结直肠黏膜组织中的表达情况,探讨两者的表达与临床病理特征之间的关系。同时探讨CFTR与E-cadherin蛋白在结直肠癌组织中的表达之间的相互关系。为进一步研究CFTR与E-cadherin蛋白在结直肠癌疾病进程中所起的作用机制以及肿瘤的预防和治疗提供理论依据支持。本实验收集解放军第309医院普通外科2012.02-2013.08手术切除的结直肠癌组织标本62例作为实验组(A组),收集相应肿瘤患者距离肿瘤组织大于5cm处正常结直肠黏膜组织6 0例作为对照组(B组)。其中A组男38例,女24例,年龄37~83之间,平均年龄55.4岁;其中高分化腺癌12例,中低分化腺癌27例,低分化腺癌23例;TNM分期:I期11例,II期26例,III期25例;其中有淋巴结转移39例,无淋巴结转移23例;有浆膜浸润的有35例,无浆膜浸润的27例。采用免疫组织化学方法(En Vision法)观察CFTR与E-cadherin蛋白在结直肠癌组织和正常结直肠黏膜组织中的表达,结果表明,CFTR及E-cadherin蛋白在结直肠癌中的表达和在正常黏膜组织相比显著降低,差异有统计学意义(P0.05)。CFTR及E-cadherin蛋白在结直肠癌中的表达具有一定的相关性,两者呈正相关(R=0.403,P0.05)。CFTR与E-cadherin蛋白表达与肿瘤的TNM分期、分化程度、浆膜浸润、淋巴结转移有关,而与患者性别、年龄及肿瘤大小无关。以上结果提示,CFTR及E-cadherin蛋白在结直肠癌发生、局部浸润和远处转移有关,两者共同参与了结直肠癌的发病过程。CFTR及E-cadherin蛋白的联合检测对于结直肠的早期诊断,预测肿瘤的侵袭和转移有重要意义,CFTR可能为治疗结直肠癌提供一个新的基因靶点和策略。
[Abstract]:Colorectal cancer is a common malignant disease of digestive tract. In our country, the morbidity and mortality of colorectal cancer are higher than the world average. Because of the late diagnosis and the high cost of treatment, the family economic burden and social burden are serious. The pathogenesis and clinical treatment of colorectal cancer are hot and difficult. In this study, we studied the expression of (cystic fibrosis transmembrane conductance regulator CFTR) and E-cadherin protein in colorectal carcinoma and normal colorectal mucosa. To explore the relationship between the expression and clinicopathological features of the two. At the same time, the relationship between the expression of CFTR and E-cadherin protein in colorectal cancer was studied. To further study the role of CFTR and E-cadherin protein in the progression of colorectal cancer, and provide theoretical basis for the prevention and treatment of tumor. In this experiment, 62 specimens of colorectal cancer were collected from the general surgery department of the 309 PLA Hospital as the experimental group (group A). 60 cases of normal colorectal mucosa from tumor tissue larger than 5cm were collected as control group (group B). In group A, there were 38 males and 24 females, aged between 3783 and 55.4 years, including 12 cases of well-differentiated adenocarcinoma, 27 cases of poorly differentiated adenocarcinoma, 23 cases of poorly differentiated adenocarcinoma, 11 cases of stage I, 26 cases of II and 25 cases of III. There were 39 cases of lymph node metastasis, 23 cases of no lymph node metastasis, 35 cases of serosal infiltration and 27 cases of no serous infiltration. The expression of CFTR and E-cadherin protein in colorectal carcinoma and normal colorectal mucosa was observed by immunohistochemical method (En Vision). The expression of CFTR and E-cadherin in colorectal cancer was significantly lower than that in normal mucosa (P0.05). The expression of). CFTR and E-cadherin was positively correlated with the TNM stage, differentiation, serosal infiltration and lymph node metastasis, but not with the sex, age and tumor size of the tumor. These results suggest that CFTR and E-cadherin proteins are involved in the pathogenesis, local invasion and distant metastasis of colorectal cancer, and both are involved in the pathogenesis of colorectal cancer. The combined detection of CFTR and E-cadherin proteins is associated with the early diagnosis of colorectal cancer. It is important to predict the invasion and metastasis of tumors. CFTR may provide a new gene target and strategy for the treatment of colorectal cancer.
【学位授予单位】：河北北方学院
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R735.34
本文编号：2423839
[Abstract]:Colorectal cancer is a common malignant disease of digestive tract. In our country, the morbidity and mortality of colorectal cancer are higher than the world average. Because of the late diagnosis and the high cost of treatment, the family economic burden and social burden are serious. The pathogenesis and clinical treatment of colorectal cancer are hot and difficult. In this study, we studied the expression of (cystic fibrosis transmembrane conductance regulator CFTR) and E-cadherin protein in colorectal carcinoma and normal colorectal mucosa. To explore the relationship between the expression and clinicopathological features of the two. At the same time, the relationship between the expression of CFTR and E-cadherin protein in colorectal cancer was studied. To further study the role of CFTR and E-cadherin protein in the progression of colorectal cancer, and provide theoretical basis for the prevention and treatment of tumor. In this experiment, 62 specimens of colorectal cancer were collected from the general surgery department of the 309 PLA Hospital as the experimental group (group A). 60 cases of normal colorectal mucosa from tumor tissue larger than 5cm were collected as control group (group B). In group A, there were 38 males and 24 females, aged between 3783 and 55.4 years, including 12 cases of well-differentiated adenocarcinoma, 27 cases of poorly differentiated adenocarcinoma, 23 cases of poorly differentiated adenocarcinoma, 11 cases of stage I, 26 cases of II and 25 cases of III. There were 39 cases of lymph node metastasis, 23 cases of no lymph node metastasis, 35 cases of serosal infiltration and 27 cases of no serous infiltration. The expression of CFTR and E-cadherin protein in colorectal carcinoma and normal colorectal mucosa was observed by immunohistochemical method (En Vision). The expression of CFTR and E-cadherin in colorectal cancer was significantly lower than that in normal mucosa (P0.05). The expression of). CFTR and E-cadherin was positively correlated with the TNM stage, differentiation, serosal infiltration and lymph node metastasis, but not with the sex, age and tumor size of the tumor. These results suggest that CFTR and E-cadherin proteins are involved in the pathogenesis, local invasion and distant metastasis of colorectal cancer, and both are involved in the pathogenesis of colorectal cancer. The combined detection of CFTR and E-cadherin proteins is associated with the early diagnosis of colorectal cancer. It is important to predict the invasion and metastasis of tumors. CFTR may provide a new gene target and strategy for the treatment of colorectal cancer.
【学位授予单位】：河北北方学院
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R735.34
【参考文献】
相关期刊论文 前1条
1 李亚秋;姜文华;范军达;郝利铭;;CFTR在肺鳞癌中的表达及其与肿瘤凋亡关系的研究[J];解剖科学进展;2013年04期
，本文编号：2423839
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