Atg3及自噬对非小细胞肺癌A549细胞迁移的影响

发布时间：2019-02-18 22:09

【摘要】：目的探讨自噬相关基因Atg3和自噬对非小细胞肺癌A549细胞迁移的影响及相关机制。方法饥饿处理诱导非小细胞肺癌A549细胞自噬,Transwell法检测细胞迁移能力的变化,Western blot检测自噬相关基因Atg3的表达。转染Atg3 si RNA靶向沉默Atg3的表达,Transwell法检测细胞迁移能力的变化、流式细胞术检测细胞凋亡、Western blot检测细胞的自噬活性以及E-cadherin、β-catenin、N-cadherin、vimentin、Notch和snail的表达。结果饥饿处理可诱导非小细胞肺癌A549细胞自噬活性增强,并提高Atg3的表达,促进细胞迁移,而不影响细胞凋亡。沉默Atg3可抑制饥饿诱导的细胞自噬及迁移,而不影响细胞凋亡。此外沉默Atg3之后,E-cadherin和β-catenin的表达明显升高,而N-cadherin、vimentin、Notch和snail的表达明显降低。结论沉默Atg3可减弱自噬活性,抑制A549细胞的迁移;其机制可能与Notch/snail信号通路活性降低进而影响细胞的EMT进程有关。
[Abstract]:Objective to investigate the effects of autophagy associated gene Atg3 and autophagy on the migration of non small cell lung cancer (NSCLC) A549 cells. Methods A549 cells were induced by starvation and the expression of autophagy related gene Atg3 was detected by Transwell assay. The expression of Atg3 was silenced by transfection of Atg3 si RNA, the changes of cell migration ability were detected by Transwell assay, the autophagy activity and E-cadherin, 尾-catenin,N-cadherin,vimentin,Notch and snail expression of apoptotic, Western blot were detected by flow cytometry. Results starvation could increase the autophagy activity of A549 cells, increase the expression of Atg3 and promote cell migration without affecting the apoptosis of A549 cells. Silencing Atg3 inhibited starvation induced autophagy and migration without affecting apoptosis. In addition, after silencing Atg3, the expression of E-cadherin and 尾-catenin increased significantly, while the expression of N-cadherin Notch and snail decreased significantly. Conclusion silencing Atg3 can decrease autophagy activity and inhibit the migration of A549 cells, and its mechanism may be related to the decrease of Notch/snail signaling pathway activity and thus the EMT process of A549 cells.
【作者单位】：南阳市中心医院呼吸内科;
【分类号】：R734.2

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