冬凌草甲素通过下调Brg1表达抑制Jurkat细胞生长
发布时间：2019-02-26 09:28
【摘要】：目的探讨冬凌草甲素对人急性T淋巴细胞白血病Jurkat细胞的作用及其机制。方法体外培养Jurkat细胞株,用不同浓度冬凌草甲素(0、1.25、2.5、5和10μmol/L)作用Jurkat细胞不同时间(24、48、72 h),MTT实验观察细胞增殖情况,荧光显微镜观察不同浓度冬凌草甲素处理Jurkat细胞12 h后的细胞核形态变化。采用Western blot半定量法检测不同浓度冬凌草甲素作用Jurkat细胞24 h,以及5μmol/L冬凌草甲素处理Jurkat细胞不同时间(0、2、6、12和24 h)后的Brg1、P53和C-myc蛋白表达。用si RNA沉默Jurkat细胞的Brg1,观察其对P53、C-myc蛋白表达以及对增殖的影响。结果与无处理组相比,冬凌草甲素对Jurkat细胞增殖有抑制作用(P0.05),且呈浓度和时间依赖性;荧光显微镜下发现,冬凌草甲素处理后的Jurkat细胞出现细胞核浓集、固缩等典型凋亡形态变化。与无处理组相比,5μmol/L冬凌草甲素作用下,Jurkat细胞的Brg1和C-myc表达下降,而P53表达上升。si RNA沉默Jurkat细胞的Brg1后,P53表达升高,C-myc蛋白表达下降,细胞生长明显受抑(P0.05)。结论冬凌草甲素对Jurkat细胞生长具有抑制作用,其机制可能通过影响Brg1信号通路起作用。
[Abstract]:Objective to investigate the effect and mechanism of Oridonin on human acute T lymphocyte leukemia (Jurkat) cells. Methods Jurkat cells were cultured in vitro. Jurkat cells were treated with different concentrations of oridonin (0, 1.25, 2.5, 5 and 10 渭 mol / L) for different time (24,48,72 h), MTT). The nuclear morphology of Jurkat cells treated with different concentrations of Oridonin for 12 h was observed by fluorescence microscope. The expression of Brg1,P53 and C-myc proteins in Jurkat cells treated with 5 渭 mol / L oridonin for 24 h (0, 2, 6, 12 and 24 h) was detected by Western blot semi-quantitative method. The effects of si RNA on the expression of P53, c-myc protein and the proliferation of Jurkat cells were observed by Brg1, silencing. Results compared with the non-treated group, oridonin inhibited the proliferation of Jurkat cells (P0.05) in a concentration-and time-dependent manner. The typical apoptotic morphological changes of Jurkat cells treated with Oridonin were observed under fluorescence microscope, such as nucleus concentration, condensation and so on. Compared with the non-treated group, the expression of Brg1 and C-myc in Jurkat cells decreased and the expression of p53 increased in Jurkat cells treated with 5 渭 mol / L oridonin, but the expression of p53 increased and the expression of C-myc protein decreased in Jurkat cells after silencing Jurkat cells with si-RNA. Cell growth was significantly inhibited (P0.05). Conclusion Aconitine can inhibit the growth of Jurkat cells, and its mechanism may play an important role in the Brg1 signaling pathway. [WT5 "HZ] conclusion [WT5" BZ]
【作者单位】： 皖南医学院弋矶山医院儿科;皖南医学院弋矶山医院放疗科;皖南医学院弋矶山医院内分泌科;
【基金】：国家自然科学基金青年项目(81600645) 皖南医学院中青年科研基金(WK2016F17)
【分类号】：R733.71
[Abstract]:Objective to investigate the effect and mechanism of Oridonin on human acute T lymphocyte leukemia (Jurkat) cells. Methods Jurkat cells were cultured in vitro. Jurkat cells were treated with different concentrations of oridonin (0, 1.25, 2.5, 5 and 10 渭 mol / L) for different time (24,48,72 h), MTT). The nuclear morphology of Jurkat cells treated with different concentrations of Oridonin for 12 h was observed by fluorescence microscope. The expression of Brg1,P53 and C-myc proteins in Jurkat cells treated with 5 渭 mol / L oridonin for 24 h (0, 2, 6, 12 and 24 h) was detected by Western blot semi-quantitative method. The effects of si RNA on the expression of P53, c-myc protein and the proliferation of Jurkat cells were observed by Brg1, silencing. Results compared with the non-treated group, oridonin inhibited the proliferation of Jurkat cells (P0.05) in a concentration-and time-dependent manner. The typical apoptotic morphological changes of Jurkat cells treated with Oridonin were observed under fluorescence microscope, such as nucleus concentration, condensation and so on. Compared with the non-treated group, the expression of Brg1 and C-myc in Jurkat cells decreased and the expression of p53 increased in Jurkat cells treated with 5 渭 mol / L oridonin, but the expression of p53 increased and the expression of C-myc protein decreased in Jurkat cells after silencing Jurkat cells with si-RNA. Cell growth was significantly inhibited (P0.05). Conclusion Aconitine can inhibit the growth of Jurkat cells, and its mechanism may play an important role in the Brg1 signaling pathway. [WT5 "HZ] conclusion [WT5" BZ]
【作者单位】： 皖南医学院弋矶山医院儿科;皖南医学院弋矶山医院放疗科;皖南医学院弋矶山医院内分泌科;
【基金】：国家自然科学基金青年项目(81600645) 皖南医学院中青年科研基金(WK2016F17)
【分类号】：R733.71
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