枸杞多糖在白血病患者中的应用及对HL-60和NK细胞杀伤活性的影响

发布时间：2019-02-27 07:31

【摘要】：目的探讨枸杞多糖在白血病患者中的应用及对人前髓细胞HL-60表面靶细胞表面相应配体(MICA)蛋白表达水平和自然杀伤(NK)细胞杀伤活性的影响及机制。方法取白血病患者HL-60细胞,随机分为观察1组、观察2组及观察3组,分别放入浓度为6、8、10 mg/ml枸杞多糖共同培养,未经枸杞多糖作用的30例白血病患者HL-60细胞为对照组,采用流式细胞仪测定4组细胞表面MICA蛋白表达水平。将4组获得的溶液与NK细胞相互混合,20 h后采用四甲基偶氮唑蓝比色(MTT)法检测NK细胞对4组HL-60细胞的杀伤活性进行测定。结果观察1组MICA表达率显著低于观察2组、观察3组(P0.05);观察1组、观察2组、观察3组MICA表达率高于对照组(P0.05);随着靶细胞、效应细胞比的不断增加,4组NK细胞杀伤活性均出现上升趋势;观察1组、观察2组及观察3组NK细胞杀伤活性高于对照组(P0.05);观察3组NK细胞杀伤活性,高于观察1组、观察2组(P0.05)。结论白血病患者体内HL-60细胞在枸杞多糖的孵育下能提高细胞MICA表达率,且呈浓度正相关性。同时,枸杞多糖能提高机体内NK细胞的杀伤活性,能清除微小病灶。
[Abstract]:Aim to investigate the application of Lycium barbarum polysaccharides in patients with leukemia and the effect of Lycium barbarum polysaccharides on the expression of (MICA) protein on the surface of target cells on the surface of human anterior myeloid cells (HL-60) and the killing activity of natural killer (NK) cells and its mechanism. Methods HL-60 cells from leukemia patients were randomly divided into observation group 1, observation group 2 and observation 3 groups. The cells were co-cultured with Lycium barbarum polysaccharides at the concentration of 6,8,10 mg/ml, respectively. The expression of MICA protein on the surface of HL-60 cells from 30 leukemia patients without Lycium barbarum polysaccharide was measured by flow cytometry. The solution obtained from the four groups was mixed with NK cells for 20 hours, and the cytotoxicity of NK cells to four groups of HL-60 cells was determined by (MTT) assay with tetramethylazo blue colorimetric assay. Results the expression rate of MICA in group 1 was significantly lower than that in group 2, and in group 3 (P0.05), the expression rate of MICA in group 1, group 2 and group 3 was higher than that in control group (P0.05). With the increasing ratio of target cells and effector cells, the killing activity of NK cells in four groups increased, and the killing activity of NK cells in group 1, group 2 and group 3 was higher than that in control group (P0.05). The killing activity of NK cells in three groups was higher than that in observation group 1 and group 2 (P0.05). Conclusion HL-60 cells incubated with Lycium barbarum polysaccharide can increase the expression rate of MICA in leukemia patients, and there is a positive correlation between the concentrations. At the same time, Lycium barbarum polysaccharide can improve the killing activity of NK cells and clear the small lesions.
【作者单位】：广州中医药大学;广东医科大学;
【基金】：广东省自然科学基金项目(No.10451008901001139)
【分类号】：R733.7

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