CD44、HIF-1α在胃癌中的表达及临床意义

发布时间：2019-03-16 16:28

【摘要】：目的：研究HIF-1α及CD44的表达与胃癌患者临床病理及预后的关系。方法：选取92例RO切除胃癌患者,用免疫组化方法研究其病理蜡块切片中HIF-1α及CD44的表达情况。对HIF-1α及CD44的表达与患者临床病理资料和随访信息进行统计分析。结果：CD44.HIF-1α在癌、癌旁、远癌组织中均有表达,在癌组织中表达率远高于后二者。HIF-1α主要表达在胞浆和胞核,CD44主要表达在细胞膜、胞浆。癌组织中二者表达高于癌旁及远癌组织,其差异具有统计学意义。HIF-1α及CD44表达与T分期(Phif-1α=0.00,Pcd44=0.01).TNM分期(Phif-1α=0.05,Pcd44=0.03)有显著相关性,此外CD44表达还与肿瘤大小(P=0.04)、淋巴结转移(P=0.02)关系密切。Spearman等级相关分析表明胃癌组织中二者的表达呈正相关(r=0.31,P=0.003).Kaplan-Meier分析显示CD44+HIF-1α+组较CD44+HIF-1α-、CD44-HIF-1α+、CD44-HIF-1α、三组生存期短,差异具统计学意义。单因素分析表明淋巴结转移(P=0.00)、T分期(P=0.00).TNM分期(P=0.00)、CD44表达(P=0.03)、HIF-1α表达(P=0.02)等因素与术后生存期相关。COX多因素回归分析显示CD44的表达(P=0.03)、淋巴结转移(P=0.01)及TNM分期(P=0.04)为胃癌患者独立预后因子。结论：①CD44. HIF-1α在癌组织中表达高于癌旁及远癌组织。②HIF-1α与CD44的表达呈正相关,二者可能相互促进表达,其具体机制尚不得知。HIF-1α与CD44双阳性表达者预后差,二者联合检测可指导胃癌患者的预后评估。③有淋巴结转移、T分期高、TNM分期高、CD44阳性表达、HIF-1α阳性表达者生存期短。其中CD44表达、淋巴结转移及TNM分期为胃癌患者术后生存的独立预后因子。
[Abstract]:Objective: to study the relationship between the expression of HIF-1 伪 and CD44 and clinical pathology and prognosis in patients with gastric cancer. Methods: the expression of HIF-1 伪 and CD44 in 92 patients with gastric cancer resected by RO were studied by immunohistochemical method. The expressions of HIF-1 伪 and CD44 were analyzed statistically with clinicopathological data and follow-up information. Results: the expression of CD44.HIF-1 伪 was found in cancer, paracancerous and distant cancer tissues, and was much higher in cancer tissues than that in the latter two tissues. CD44 was mainly expressed in cytoplasm and nucleus, and CD44 was mainly expressed in cell membrane and cytoplasm. The expression of HIF-1 伪 and CD44 was significantly correlated with T stage (Phif-1 伪 = 0.05, Pcd44 / 0.003), and the expression of HIF-1 伪 and HIF-1 伪 were significantly correlated with T stage (Phif-1 伪 = 0.05, Pcd44 / 0.003), and the expression of HIF-1 伪 and HIF-1 伪 were significantly correlated with T stage (P < 0.05). The expression of CD44 was positively correlated with tumor size (P < 0. 04) and lymph node metastasis (P < 0. 02) in gastric cancer tissues (r = 0. 31, P < 0. 01, P < 0. 01). Kaplan-Meier analysis showed that the survival time of CD44 HIF-1 伪 group was shorter than that of CD44 HIF-1 伪, CD44-HIF-1 伪, and the difference was statistically significant. Univariate analysis showed lymph node metastasis (P < 0. 00), T stage), TNM stage (P < 0. 00) and CD44 expression (P < 0. 03). Cox multivariate regression analysis showed that the expression of CD44, lymph node metastasis and TNM stage were independent prognostic factors in patients with gastric cancer. Conclusion: 1. The expression of HIF-1-1 伪 in cancer tissues was higher than that in paracancerous and distant cancer tissues. 2HIF-1 伪 expression was positively correlated with CD44 expression, which might promote the expression of HIF-1 伪. The specific mechanism of the expression of HIF-1 伪 and HIF-1 伪 was not yet known. The patients with double positive expression of CD44 and HIF-1 伪 had poor prognosis. 3) the patients with lymph node metastasis, high T stage, high TNM stage, positive expression of CD44 and HIF-1 伪 had shorter survival time. CD44 expression, lymph node metastasis and TNM stage were independent prognostic factors for postoperative survival of patients with gastric cancer.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R735.2

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