RASSF1A与CyclinA2在原发性胆囊癌中的表达意义和相关性

发布时间：2019-03-27 15:40

【摘要】：目的:研究在原发性胆囊癌中RASSF1A与Cyclin A2的表达,与临床生物学行为之间的关系,以及探讨这二者之间相关性。方法:收集2007-03—2015-09青海省人民医院手术切除的40例原发性胆囊癌和40例慢性胆囊炎(胆囊结石术后经病理证实)组织标本,检测在上述两种组织中的RASSF1A与Cyclin A2表达(免疫组化三步法),统计两者的表达状况,分析有无显著性差异性,并计算两者有无相关性。结果:RASSF1A和Cyclin A2分别在实验和对照组中的表达有显著性差异(P0.05),且二者在PGC的表达具有相关性(C=0.398)。RASSF1A及Cyclin A2在患者的年龄、性别,包括癌细胞分化方面的表达均无明显差异。而在脏器及淋巴结转移、Nevin和TNM分期中差异明显。结论:(1)RASSF1A与CyclinA2蛋白异常表达可能参与胆囊的恶变过程。(2)RASSF1A与Cyclin A2蛋白在胆囊癌中呈现负相关关系,可能两者间存在负反馈调节机制。(3)RASSF1A与Cyclin A2蛋白与胆囊癌的关系,为原发性胆囊癌的发生、发展、预后及治疗方式的选择提供参考。
[Abstract]:Aim: to study the expression of RASSF1A and Cyclin A2 in primary gallbladder carcinoma and its relationship with clinical biological behavior. Methods: 40 cases of primary gallbladder carcinoma and 40 cases of chronic cholecystitis (pathologically proved by pathology after operation) were collected from 40 cases of primary gallbladder carcinoma and 40 cases of chronic cholecystitis (confirmed by pathology after operation) in Qinghai Provincial people's Hospital in 2007 / 03 / 2015 / 09. To detect the expression of RASSF1A and Cyclin A2 in the above two tissues (three-step immunohistochemical method), to statistics the expression status of the two tissues, to analyze the significant difference between the two tissues, and to calculate the correlation between the two tissues. Results: there was a significant difference in the expression of RASSF1A and Cyclin A2 between the experimental and control groups (P0.05), and the expression of RASSF1A and Cyclin A2 in PGC were correlated with each other (C = 0.398). RASSF1A and RASSF A2 were correlated with the age and sex of the patients. There was no significant difference in expression in differentiation of cancer cells. There was significant difference between Nevin and TNM stages in organ and lymph node metastasis. Conclusion: (1) the abnormal expression of RASSF1A and CyclinA2 proteins may be involved in the malignant process of gallbladder. (2) there is a negative correlation between RASSF1A and CyclinA2 protein in gallbladder carcinoma. (3) the relationship between RASSF1A and Cyclin A2 protein and gallbladder carcinoma provides a reference for the occurrence, development, prognosis and treatment of primary gallbladder carcinoma.
【学位授予单位】：青海大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R735.8

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