Ulk2过表达对非小细胞肺癌细胞系增殖和化疗敏感性的影响
发布时间：2019-04-02 01:51
【摘要】：第一部分 Ulk2过表达对肺癌细胞细胞系A549、H460自噬水平的影响目的：研究Ulk2过表达对非小细胞肺癌细胞系(A549和H460)细胞自噬水平的影响。方法：培养A549和H460细胞,将表达Ulk2过表达质粒和空载质粒转染A549和H460细胞,使用western blot验证质粒转染结果,并检测细胞自噬相关标记物LC3 Ⅱ和p62的表达量。结果：转染Ulk2过表达质粒后,两种细胞Ulk2的表达量相对转染空载质粒的对照组提升,并且细胞自噬相关标记物LC3 Ⅱ表达量明显升高,p62的表达量明显降低,结果具有统计学意义(p0.05)。结论：转染Ulk2过表达质粒可以增强非小细胞肺癌细胞系(A549和H460)细胞自噬水平。第二部分 UIk2过表达对肺癌细胞的增殖能力、对药物敏感性及细胞凋亡的影响目的：研究Ulk2过表达对非小细胞肺癌细胞系(A549和H460)增殖及化疗敏感性的影响。方法：培养A549和H460细胞,将表达Ulk2过表达质粒和空载质粒转染A549和H460细胞,MTT法检测细胞增殖和对化疗药物的敏感性,流式细胞术和Western blot检测细胞凋亡。结果：与对照组相比,Ulk2过表达后A549和H460的细胞增殖能力明显降低(P0.05),对顺铂和依托泊苷的敏感性明显上升(P0.05),顺铂处理后细胞凋亡比率增加,切割后的Caspase3和PARP明显升高。结论：U1k2过表达能显著抑制非小细胞肺癌细胞系增殖,增强化疗敏感性。
[Abstract]:Part one: effects of Ulk2 overexpression on autophagy in lung cancer cell lines A549 and H460 objective: to study the effect of Ulk2 overexpression on autophagy in non-small cell lung cancer cell lines A549 and H460. Methods: A549 and H460 cells were cultured and transfected with Ulk2 overexpression plasmid and empty plasmid. The results were verified by western blot, and the expression levels of LC3 鈪，
本文编号：2452118
[Abstract]:Part one: effects of Ulk2 overexpression on autophagy in lung cancer cell lines A549 and H460 objective: to study the effect of Ulk2 overexpression on autophagy in non-small cell lung cancer cell lines A549 and H460. Methods: A549 and H460 cells were cultured and transfected with Ulk2 overexpression plasmid and empty plasmid. The results were verified by western blot, and the expression levels of LC3 鈪，
本文编号：2452118
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