MicroRNA-126与胃癌患者放化综合治疗效果的关系及其对胃癌SGC-7901细胞的放射增敏作用

发布时间：2019-04-11 14:25

【摘要】：目的:研究microRNA-126与胃癌患者放化综合治疗疗效的关系及其对胃癌细胞的放射增敏效果。方法:选取2014年6月~2015年6月间我院收治的晚期胃癌患者60例为研究对象,其中男性32例,女性28例,年龄37~65岁,平均年龄(51±7)岁。所有患者均接受放化综合治疗。收集患者的胃癌病理组织标本,同时于治疗结束时采集静脉血标本。采用国际实体瘤疗效评价标准(RECIST)评价患者的近期疗效,根据疗效将患者分为治疗敏感组和非敏感组。实时荧光定量PCR检测各组患者组织及血浆中microRNA-126的表达变化。体外培养胃癌SGC-7901细胞并转染microRNA-126 mimic;平板集落形成实验分析microRNA-126 mimic转染对SGC-7901细胞放射敏感性的影响;流式细胞术检测转染microRNA-126 mimic对SGC-7901细胞凋亡率的影响。结果:根据RECIST,共28例患者对治疗敏感,32例患者对治疗不敏感。与敏感组患者相比,不敏感组患者血浆和胃癌组织中的microRNA-126相对水平较低,相对于敏感组的倍数分别为0.72±0.04和0.48±0.03,差异具有统计学意义(P0.05)。MicroRNA-126 mimic转染后SGC-7901细胞的SF_2值和D_0值减小(P0.05),增敏比为1.74。流式细胞术分析结果发现,microRNA-126 mimic转染可以诱导SGC-7901细胞凋亡,并增加射线引起的细胞凋亡。结论:较低的microRNA-126提示胃癌患者放化综合治疗疗效不佳;microRNA-126具有增加胃癌细胞放射敏感性的作用。
[Abstract]:Objective: to study the relationship between microRNA-126 and radiotherapy in patients with gastric cancer and its radiosensitizing effect on gastric cancer cells. Methods: from June 2014 to June 2015, 60 patients with advanced gastric cancer were selected, including 32 males and 28 females with an average age of (51 卤7) years. All patients were treated with radiotherapy and chemotherapy. Pathological specimens of gastric cancer were collected and venous blood samples were collected at the end of treatment. The patients were divided into sensitive group and non-sensitive group according to the curative effect. (RECIST) was used to evaluate the short-term curative effect of the patients. Real-time fluorescence quantitative PCR was used to detect the expression of microRNA-126 in tissues and plasma of each group. SGC-7901 cells were cultured in vitro and transfected with microRNA-126 mimic; plate colony forming assay to analyze the effect of microRNA-126 mimic transfection on the radiosensitivity of SGC-7901 cells, and flow cytometry was used to detect the effect of microRNA-126 mimic transfection on the apoptosis rate of SGC-7901 cells. Results: according to RECIST, 28 patients were sensitive to treatment and 32 patients were insensitive to treatment. Compared with the sensitive group, the relative level of microRNA-126 in plasma and gastric cancer tissue in the insensitive group was lower than that in the sensitive group, which was 0.72 卤0.004 and 0.48 卤0.03, respectively. The SF_-2 and D0 values of SGC-7901 cells transfected with MicroRNA-126 mimic were decreased (P0.05), and the sensitizing ratio was 1.74. The results of flow cytometry showed that microRNA-126 mimic transfection could induce apoptosis of SGC-7901 cells and increase radiation-induced apoptosis. Conclusion: the low microRNA-126 suggested that the combined radiotherapy and chemotherapy therapy was not effective in patients with gastric cancer, and microRNA-126 could increase the radiosensitivity of gastric cancer cells.
【作者单位】：甘肃省肿瘤医院放疗一病区;
【分类号】：R735.2

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