miRNA-141通过抑制MAP2K4促进大肠癌增殖的研究
发布时间：2019-04-15 22:34
【摘要】：大肠癌(colorectal cancer,CRC)是最常见的消化系统恶性肿瘤之一,在全球范围内位居女性恶性肿瘤发病率的第2位,男性恶性肿瘤发病率的第3位。大肠癌为第5位恶性肿瘤致死病种,患者的生存和预后与大肠癌的首次诊断时间,治疗方法以及对化疗的敏感性等密切相关。因此,及时有效地诊断和治疗大肠癌患者具有重要意义。微小RNA(micro RNA,mi RNA)是一种单链非编码小分子RNA,长度为18~25个核苷酸,它可通过与靶m RNA 3’末端非翻译区完全或不完全匹配结而诱导靶m RNA降解或阻遏其翻译,从而在转录后水平沉默基因的表达,进而发挥其功能。目前,已在哺乳动物中发现超过1000种,经研究确认的mi RNA有533种。进而使人们对基因表达的调控有了新的认识。mi RNA在多种生物学过程,如细胞生长、增殖、分化和凋亡等发挥着重要的作用。mi RNA涉及多种疾病,包括心血管系统、神经系统、免疫系统疾病和癌症。mi RNA在多种恶性肿瘤中异常表达,被证明参与肿瘤的发生、进展、侵袭、转移及血管生成等。在大肠癌中,mi RNA的异常表达与肿瘤的大小、分期和预后等均明显相关,并可能为大肠癌的早期诊断提供新的分子标志物。微小RNA(mi RNA)可以作为肿瘤抑制或致癌基因。研究表明mi RNA-141水平在大肠癌组织样本中上调。实验观察到很多mi R-14的可能靶目标,包括肿瘤抑制基因MAP2K4。本研究的目的是探讨mi R-141促进大肠癌增殖的作用。方法采用RT-PCR检测20例确诊结肠腺癌及其配对癌周正常组织中mi R-141以及MAP2K4的m RNA表达水平;分析mi R-141与TNM分期的相关性,利用mi R-141 inhibitor或mi R-141 mimics分析抑制或者增加细胞中mi R-141表达对结肠腺癌细胞系增殖的影响,利用si RNA抑制MAP2K4表达,明确MAP2K4蛋白在mi R-141引起的大肠癌细胞增殖中的作用。免疫组化染色检测大肠癌组织及癌周正常组织MAP2K4蛋白表达。Western blot检测大肠癌细胞中MAP2K4蛋白水平。利用MTT法检测大肠癌细胞增殖情况。结果miR-141在TNMⅢ+Ⅳ期的mi R-141 m RNA表达水平显著高于Ⅰ+Ⅱ期。与癌旁正常组织相比,20例大肠癌临床样品中的mi R-141水平均显著上调。与此相反,MAP2K4的表达水平均显著下降。通过MTT细胞增殖测定实验,过度表达mi R-141通过抑制MAP2K4活性导致大肠癌细胞增殖。结论miR-141与TNM分期相关,mi R-141上调与大肠癌细胞增殖有关,mi R-141是通过抑制肿瘤抑制基因MAP2K4而促进癌细胞的生长。针对mi R-141的策略可以提供一个有效治疗大肠癌的方法。
[Abstract]:Colorectal cancer (colorectal cancer,CRC) is one of the most common malignant tumors of digestive system. It is the second most common malignant tumor in female and the third in male. Colorectal cancer is the fifth leading cause of death from malignant tumors. The survival and prognosis of the patients are closely related to the first diagnosis time, treatment methods and sensitivity to chemotherapy of colorectal cancer. Therefore, timely and effective diagnosis and treatment of colorectal cancer patients have important significance. Small RNA (micro RNA,mi RNA) is a single-stranded non-coding small molecule with a length of 18 渭 25 nucleotides. It can induce the degradation of target m-RNA or inhibit its translation by completely or incompletely matching the untranslated region at the 3 'end of the target m RNA. Thus silencing the expression of the gene at post-transcriptional level, and then play its role. At present, more than 1000 species of mi RNA have been found in mammals and 533 species have been identified. Mi RNA plays an important role in many biological processes, such as cell growth, proliferation, differentiation and apoptosis. Mi RNA involves many diseases, including cardiovascular system, nervous system, and so on. The abnormal expression of. Mi RNA in many kinds of malignant tumors has been proved to be involved in tumorigenesis, progression, invasion, metastasis, angiogenesis and so on. The abnormal expression of, mi RNA is significantly correlated with the size, stage and prognosis of colorectal cancer, and may provide a new molecular marker for early diagnosis of colorectal cancer. Tiny RNA (mi RNA) can be used as a tumor suppressor or oncogene. Studies have shown that mi RNA-141 levels are up-regulated in colorectal cancer tissue samples. A number of possible target targets of mi RX14, including the tumor suppressor gene MAP2K4., were observed in the experiment. The purpose of this study was to investigate the role of mi Rc 141 in promoting the proliferation of colorectal cancer. Methods RT-PCR was used to detect the mRNA expression of mi-ru-141 and MAP2K4 in 20 cases of colorectal adenocarcinoma and their matched surrounding normal tissues. To analyze the correlation between mi rm 141 and TNM stage, to analyze the effect of inhibiting or increasing the expression of mi rm 141 on the proliferation of colorectal adenocarcinoma cell line by mi Rc 141 inhibitor or mi Rm 141 mimics, and to inhibit the expression of MAP2K4 by si RNA, and the expression of MAP2K4 in colorectal adenocarcinoma cell line was inhibited by si RNA. To investigate the role of MAP2K4 protein in the proliferation of colorectal cancer cells induced by mi Rc 141. Immunohistochemical staining was used to detect the expression of MAP2K4 protein and Western blot was used to detect the expression of MAP2K4 protein in colorectal cancer tissues and normal tissues. The proliferation of colorectal cancer cells was detected by MTT method. Results the expression level of miR-141 m RNA of miR-141 in TNM 鈪，
本文编号：2458544
[Abstract]:Colorectal cancer (colorectal cancer,CRC) is one of the most common malignant tumors of digestive system. It is the second most common malignant tumor in female and the third in male. Colorectal cancer is the fifth leading cause of death from malignant tumors. The survival and prognosis of the patients are closely related to the first diagnosis time, treatment methods and sensitivity to chemotherapy of colorectal cancer. Therefore, timely and effective diagnosis and treatment of colorectal cancer patients have important significance. Small RNA (micro RNA,mi RNA) is a single-stranded non-coding small molecule with a length of 18 渭 25 nucleotides. It can induce the degradation of target m-RNA or inhibit its translation by completely or incompletely matching the untranslated region at the 3 'end of the target m RNA. Thus silencing the expression of the gene at post-transcriptional level, and then play its role. At present, more than 1000 species of mi RNA have been found in mammals and 533 species have been identified. Mi RNA plays an important role in many biological processes, such as cell growth, proliferation, differentiation and apoptosis. Mi RNA involves many diseases, including cardiovascular system, nervous system, and so on. The abnormal expression of. Mi RNA in many kinds of malignant tumors has been proved to be involved in tumorigenesis, progression, invasion, metastasis, angiogenesis and so on. The abnormal expression of, mi RNA is significantly correlated with the size, stage and prognosis of colorectal cancer, and may provide a new molecular marker for early diagnosis of colorectal cancer. Tiny RNA (mi RNA) can be used as a tumor suppressor or oncogene. Studies have shown that mi RNA-141 levels are up-regulated in colorectal cancer tissue samples. A number of possible target targets of mi RX14, including the tumor suppressor gene MAP2K4., were observed in the experiment. The purpose of this study was to investigate the role of mi Rc 141 in promoting the proliferation of colorectal cancer. Methods RT-PCR was used to detect the mRNA expression of mi-ru-141 and MAP2K4 in 20 cases of colorectal adenocarcinoma and their matched surrounding normal tissues. To analyze the correlation between mi rm 141 and TNM stage, to analyze the effect of inhibiting or increasing the expression of mi rm 141 on the proliferation of colorectal adenocarcinoma cell line by mi Rc 141 inhibitor or mi Rm 141 mimics, and to inhibit the expression of MAP2K4 by si RNA, and the expression of MAP2K4 in colorectal adenocarcinoma cell line was inhibited by si RNA. To investigate the role of MAP2K4 protein in the proliferation of colorectal cancer cells induced by mi Rc 141. Immunohistochemical staining was used to detect the expression of MAP2K4 protein and Western blot was used to detect the expression of MAP2K4 protein in colorectal cancer tissues and normal tissues. The proliferation of colorectal cancer cells was detected by MTT method. Results the expression level of miR-141 m RNA of miR-141 in TNM 鈪，
本文编号：2458544
本文链接：https://www.wllwen.com/yixuelunwen/zlx/2458544.html
