叶酸在MNNG致哈萨克族食管上皮APE1、MGMT基因表达改变中的作用

发布时间：2019-04-17 12:17

【摘要】：目的探讨叶酸在N-甲基-N-硝基-N-亚硝基胍(MNNG)致哈萨克族食管上皮细胞APE1、MGMT基因mRNA及蛋白表达中的作用。方法采用3×3析因设计将体外培养的哈萨克族食管上皮细胞暴露于不同浓度叶酸(0.00、0.40和0.80μg/ml)和MNNG(0.00、0.75和1.50μg/ml)的培养液中作用48、72和96 h后,采用RT-PCR法检测APE1、MGMT基因mRNA的表达水平,采用Western Blot法检测APE1、MGMT基因蛋白表达水平。结果随着叶酸浓度降低和MNNG浓度增加,APE1基因的mRNA及蛋白表达水平随着损伤的增加而增加,差异有统计学意义(P0.01);MGMT基因的mRNA及蛋白表达水平则随损伤的增加而下降,差异有统计学意义(P0.01)。结论叶酸浓度降低对MNNG致哈萨克族食管上皮细胞损伤有一定的促进作用,而保持充足的叶酸则对MNNG的损害起到一定的保护作用。
[Abstract]:Aim to investigate the role of folic acid in the expression of APE1,MGMT gene mRNA and protein in Kazakh esophageal epithelial cells induced by N-methyl-N-nitro-N-nitrosoguanidine (N-methyl-N-nitrosoguanidine) (MNNG). Methods Kazakh esophageal epithelial cells were exposed to different concentrations of folic acid (0.00,0.40,0.80ug / ml) and MNNG (0.00,0.75,1.50ug / ml) for 48, 72 and 96 h after exposure to 3 脳 3 factorial design. The expression level of APE1,MGMT gene mRNA was detected by RT-PCR and the expression level of APE1,MGMT gene protein was detected by Western Blot method. Results with the decrease of folic acid concentration and the increase of MNNG concentration, the expression level of mRNA and protein of APE1 gene increased with the increase of injury, the difference was statistically significant (P0.01). The expression level of mRNA and protein of MGMT gene decreased with the increase of injury (P0.01). Conclusion the decrease of folic acid concentration may promote the damage of Kazak esophageal epithelial cells induced by MNNG, while maintaining adequate folic acid may have a protective effect on the damage of MNNG.
【作者单位】：浙江省嘉兴学院医学院;新疆医科大学公共卫生学院卫生毒理学教研室;
【基金】：国家自然科学基金(81460502) 新疆维吾尔自治区科技厅自然科学基金(2015211C032)
【分类号】：R735.1

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