miRNA-199a-3p调控MAP3K4在胃癌中的表达
发布时间：2019-04-19 03:53
【摘要】：目的探讨胃癌中MIRNA-199A-3P调控候选基因MAP3K4的表达。方法收集35例胃癌及癌旁组织标本,采用茎环逆转录-聚合酶链式反应法检测胃癌组织及癌旁组织中MIR-199A-3P的表达水平,WESTERN BLOT法检测MAP3K4表达;细胞转染法检测MIR-199A-3P调控MAP3K4表达,双荧光素酶报告基因检测系统验证MIR-199A-3P结合MAP3K4的3’UTR的靶位点。结果与癌旁组织相比,胃癌组织MIR-199A-3P高表达,且其候选基因MAP3K4低表达;MIR-199A-3P MIMICS转染抑制MAP3K4表达;MAP3K4是MIR-199A-3P作用的靶基因。结论 MIR-199A-3P是胃癌的一个癌基因,MAP3K4是受其调控的靶基因。
[Abstract]:Objective to investigate the expression of MIRNA-199A-3P regulatory candidate gene MAP3K4 in gastric cancer. Methods 35 specimens of gastric cancer and adjacent tissues were collected. The expression of MIR-199A-3P in gastric cancer and adjacent tissues was detected by, WESTERN BLOT method. The expression of MAP3K4 in gastric cancer tissues and adjacent tissues was detected by reverse transcription-polymerase chain reaction (RT-PCR). Cell transfection assay was used to detect the expression of MAP3K4 regulated by MIR-199A-3P and double luciferase reporter gene detection system was used to verify the target site of 3'UTR binding to MAP3K4 by MIR-199A-3P. Results compared with the paracancerous tissues, MIR-199A-3P was highly expressed and its candidate gene MAP3K4 was low; MIR-199A-3P MIMICS transfection inhibited the expression of MAP3K4; MAP3K4 was the target gene of MIR-199A-3P. Conclusion MIR-199A-3P is a oncogene of gastric cancer, and MAP3K4 is a target gene regulated by it.
【作者单位】： 华中科技大学同济医学院附属普爱医院检验科;
【基金】：武汉市卫计委科研基金资助项目(编号:WX13C15)
【分类号】：R735.2
本文编号：2460576
[Abstract]:Objective to investigate the expression of MIRNA-199A-3P regulatory candidate gene MAP3K4 in gastric cancer. Methods 35 specimens of gastric cancer and adjacent tissues were collected. The expression of MIR-199A-3P in gastric cancer and adjacent tissues was detected by, WESTERN BLOT method. The expression of MAP3K4 in gastric cancer tissues and adjacent tissues was detected by reverse transcription-polymerase chain reaction (RT-PCR). Cell transfection assay was used to detect the expression of MAP3K4 regulated by MIR-199A-3P and double luciferase reporter gene detection system was used to verify the target site of 3'UTR binding to MAP3K4 by MIR-199A-3P. Results compared with the paracancerous tissues, MIR-199A-3P was highly expressed and its candidate gene MAP3K4 was low; MIR-199A-3P MIMICS transfection inhibited the expression of MAP3K4; MAP3K4 was the target gene of MIR-199A-3P. Conclusion MIR-199A-3P is a oncogene of gastric cancer, and MAP3K4 is a target gene regulated by it.
【作者单位】： 华中科技大学同济医学院附属普爱医院检验科;
【基金】：武汉市卫计委科研基金资助项目(编号:WX13C15)
【分类号】：R735.2
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