泮托拉唑改善小鼠肿瘤恶病质骨骼肌消耗

发布时间：2019-04-19 23:25

【摘要】：【目的】研究泮托拉唑对肿瘤恶病质骨骼肌消耗的缓解作用。【方法】将24只BALB/c小鼠随机分为3组,分别为正常组(NN)、恶病质组(CC)、干预组(PPI),每组8只。荷瘤小鼠采用腋下接种小鼠结肠癌细胞(c26),建立肿瘤恶病质模型。每日监测各组小鼠体质量、肿瘤大小及精神毛发等一般情况。接种后第12天干预组采取泮托拉唑灌胃,其余组采取生理盐水灌胃。干预后第12天处死小鼠收集标本。称量腓肠肌及肿瘤质量,测量肿瘤大小,HE染色观察骨骼肌形态变化,血清ELISA法检测IL-6、TNF-α水平,qRT-PCR和Werstern blot分别检测腓肠肌MuRF1、MAFBx、Myod1和myf5的mRNA和蛋白表达水平。【结果】与CC组相比,PPI组小鼠及腓肠肌质量分别增加39.8%和24.2%,腓肠肌纤维横切面积增加25.4%;PPI组Myod1和myf5的mRNA水平及蛋白表达水平明显高于CC组(P0.05);PPI组血清IL-6及TNF-α水平较CC组分别降低30.7%和18.9%;PPI组MuRF1、MAFBx蛋白表达水平明显低于CC组(P0.05)。【结论】泮托拉唑对肿瘤恶病质骨骼肌消耗具有保护作用,其机制可能与抑制炎症及泛素蛋白酶体途径(UPS),上调生肌调节因子(MRF)有关。
[Abstract]:[objective] to study the effect of pantoprazole on skeletal muscle consumption of tumor cachexia. [methods] Twenty-four BALB/c mice were randomly divided into 3 groups: normal group, (NN), cachexia group, (CC), intervention group, (PPI), group, 8 mice in each group. The tumor cachexia model was established by subaxillary inoculation of colon cancer cells (c26) in tumor-bearing mice. The body mass, tumor size and mental hair of each group were monitored daily. On the 12th day after inoculation, pantoprazole was administered intragastrically in the intervention group and normal saline was given to the other groups. The mice were killed on the 12th day after intervention. Weigh the gastrocnemius muscle and tumor mass, measure the tumor size, observe the morphological changes of skeletal muscle by HE staining, detect the level of IL-6,TNF- 伪 by serum ELISA method, and detect the MuRF1,MAFBx, of gastrocnemius muscle by qRT-PCR and Werstern blot respectively. [results] compared with CC group, the mass of gastrocnemius muscle and gastrocnemius muscle in PPI group increased by 39.8% and 24.2%, and the cross-sectional area of gastrocnemius muscle fiber increased by 25.4%; The levels of mRNA and protein in Myod1 and myf5 in PPI group were significantly higher than those in CC group (P0.05). The levels of IL-6 and TNF- 伪 in serum in); PPI group were lower than those in CC group by 30.7% and 18.9%, respectively. [conclusion] pantoprazole has protective effect on skeletal muscle consumption of tumor cachexia, which may be related to the inhibition of inflammation and ubiquitin proteasome pathway (UPS),. [conclusion] Pantoprazole has protective effect on skeletal muscle consumption of cachexia in PPI group compared with CC group (P0.05). The upregulation of myogenic regulatory factor (MRF) is related.
【作者单位】：重庆医科大学附属第一医院胃肠外科;
【基金】：重庆市研究生科研创新项目(CYS15135)
【分类号】：R730.5

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