血清标志物在食管鳞癌早期诊断中的应用研究
发布时间：2019-05-18 23:28
【摘要】：国处于全球食管癌的高发地区,食管鳞癌发病率和死亡率在我国分别居恶性肿瘤的第五和第四位,极大影响着我国人民的健康。食管鳞癌具有起病隐匿、进展快、侵袭性强等特点,确诊时往往已处于晚期。食管鳞癌的总体五年生存率仅20%左右,而如果能早期发现和治疗,Ⅰ期食管鳞癌的五年生存率可以达到70-80%。因此若能找到有效的早期发现方法,是提高食管鳞癌整体预后的根本措施。当今常用的筛查手段,诸如胃镜、X线造影等,存在有创、操作复杂、检测周期长、敏感性低等缺点,不适于推广至大范围人群。血清标志物则具有操作简便、相对无创、经济、快速等优势,适合作为广泛人群筛查的手段。本项研究试图寻找早期诊断食管鳞癌的血清标志物组合,以提高食管鳞癌的早期诊断率,进而改善该病的整体预后水平。研究取用食管鳞癌患者血清和健康人血清分别作为实验组和对照组样本。选取近年来文献报道效果较突出的7种分泌蛋白标志物：CEA、Cyfra21-1、SCC、DKK-1、 MIC-1、HGF和LY6K,用ELISA方法首先进行小样本(n=50)检测,筛选出4个因子CEA、Cyfra21-1、SCC和MIC-1后,进一步进行了较大样本(n=196)血清标志物联合分析。结果显示,4种标志物联合检测食管鳞癌的敏感性、特异性、准确性分别为75.0%、65.8%、70.4%,优于任何一种标志物单一检测的诊断效率。另外,研究还尝试检测了自身血清P53抗体标志物。经过摸索实验条件,初步建立了检测自身抗体的实验方案。小规模(n=25)检测结果显示,P53抗体在食管鳞癌患者血清中表达水平显著高于正常对照。通过本项研究,初步探究了早期诊断食管鳞癌的血清标志物,获得了优于现有标志物的新标志物组合,具有潜在的临床应用前景。
[Abstract]:China is in a high incidence of esophageal cancer in the world, the incidence and mortality of esophageal squamous cell carcinoma ranks fifth and fourth in China respectively, which greatly affects the health of our people. Esophageal squamous cell carcinoma has the characteristics of hidden onset, rapid progress and strong invasiveness, and is often in the late stage at the time of diagnosis. The overall five-year survival rate of esophageal squamous cell carcinoma was only about 20%. If early detection and treatment, the 5-year survival rate of stage I esophageal squamous cell carcinoma could reach 70% 80%. Therefore, if we can find an effective early detection method, it is the fundamental measure to improve the overall prognosis of esophageal squamous cell carcinoma. Nowadays, the commonly used screening methods, such as gastroscopy, X-ray radiography and so on, have some shortcomings, such as invasive, complex operation, long detection cycle, low sensitivity and so on, so they are not suitable for wide population. Serum markers have the advantages of simple operation, non-invasive, economical and rapid, and are suitable for screening a wide range of people. This study attempts to find a combination of serum markers for early diagnosis of esophageal squamous cell carcinoma in order to improve the early diagnosis rate of esophageal squamous cell carcinoma and thus improve the overall prognosis of the disease. The serum of patients with esophageal squamous cell carcinoma and healthy human serum were taken as experimental group and control group, respectively. Seven secretory protein markers reported in the literature in recent years were selected: CEA,Cyfra21-1,SCC,DKK-1, MIC-1,HGF and LY6K, were first detected by ELISA method (n 鈮，
本文编号：2480426
[Abstract]:China is in a high incidence of esophageal cancer in the world, the incidence and mortality of esophageal squamous cell carcinoma ranks fifth and fourth in China respectively, which greatly affects the health of our people. Esophageal squamous cell carcinoma has the characteristics of hidden onset, rapid progress and strong invasiveness, and is often in the late stage at the time of diagnosis. The overall five-year survival rate of esophageal squamous cell carcinoma was only about 20%. If early detection and treatment, the 5-year survival rate of stage I esophageal squamous cell carcinoma could reach 70% 80%. Therefore, if we can find an effective early detection method, it is the fundamental measure to improve the overall prognosis of esophageal squamous cell carcinoma. Nowadays, the commonly used screening methods, such as gastroscopy, X-ray radiography and so on, have some shortcomings, such as invasive, complex operation, long detection cycle, low sensitivity and so on, so they are not suitable for wide population. Serum markers have the advantages of simple operation, non-invasive, economical and rapid, and are suitable for screening a wide range of people. This study attempts to find a combination of serum markers for early diagnosis of esophageal squamous cell carcinoma in order to improve the early diagnosis rate of esophageal squamous cell carcinoma and thus improve the overall prognosis of the disease. The serum of patients with esophageal squamous cell carcinoma and healthy human serum were taken as experimental group and control group, respectively. Seven secretory protein markers reported in the literature in recent years were selected: CEA,Cyfra21-1,SCC,DKK-1, MIC-1,HGF and LY6K, were first detected by ELISA method (n 鈮，
本文编号：2480426
本文链接：https://www.wllwen.com/yixuelunwen/zlx/2480426.html
